Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology
Context In China, HUANGQI is widely used for the treatment of Alzheimer’s disease (AD). However, a comprehensive understanding of its mechanism of anti-AD effects is lacking.Objective To explore the active ingredients of HUANGQI and its potential targets and mechanisms of action in AD.Materials and...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2024.2382962 |
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| author | Feng Lv Mei Sun Chunmeng Qin Dan Du Xiangru Zheng Wenjun Li |
| author_facet | Feng Lv Mei Sun Chunmeng Qin Dan Du Xiangru Zheng Wenjun Li |
| author_sort | Feng Lv |
| collection | DOAJ |
| description | Context In China, HUANGQI is widely used for the treatment of Alzheimer’s disease (AD). However, a comprehensive understanding of its mechanism of anti-AD effects is lacking.Objective To explore the active ingredients of HUANGQI and its potential targets and mechanisms of action in AD.Materials and methods The active ingredients and targets of HUANGQI were screened from databases (TCSMP, ETCM, and BATMan), and AD-related genes were obtained from DrugBank and GeneCards. The same target genes were screened, and a drug-target disease network was constructed. The PPI network was constructed and GO and KEGG pathway enrichment analyses of the targets. The Cell Counting Kit-8 (CCK-8) assay was used to determine suitable HUANGQI treatment concentrations for HT-22 cells between 0-480 μg/mL. CCK-8, FITC-phalloidin and propidium iodide (PI) assays were used to examine the protective effect of (0, 60, 120, 240 μg/mL) of HUANGQI on 20 μM Aβ1-42-induced HT-22 cell cytotoxicity.Results Twelve active ingredients of HUANGQI were selected, with 679 common targets associated with AD. GO and KEGG analysis revealed that the therapeutic mechanisms of HUANGQI involve TNF, AGE, the NF-κB pathway, and nuclear receptor activity-related processes. The CCK-8 assay indicated that HUANGQI was not cytotoxic to HT-22 cells at concentrations less than 240 μg/mL and was able to attenuate Aβ1-42-induced cellular damage (EC50 = 83.46 μg/mL). FITC-phalloidin and PI assays suggested that HUANGQI could alleviate 20 μM Aβ1-42-induced neuronal cell cytotoxicity in a dose-dependent manner.Conclusion HUANGQI has a protective effect on Aβ1-42-induced nerve cell injury; further mechanism research was needed. |
| format | Article |
| id | doaj-art-9da1e2e3ad4e46718cbf58deffecd164 |
| institution | Kabale University |
| issn | 1388-0209 1744-5116 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj-art-9da1e2e3ad4e46718cbf58deffecd1642024-12-09T07:41:45ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162024-12-0162163464710.1080/13880209.2024.2382962Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technologyFeng Lv0Mei Sun1Chunmeng Qin2Dan Du3Xiangru Zheng4Wenjun Li5Department of Pharmacy, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacy, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacy, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacy, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Pharmacy, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaContext In China, HUANGQI is widely used for the treatment of Alzheimer’s disease (AD). However, a comprehensive understanding of its mechanism of anti-AD effects is lacking.Objective To explore the active ingredients of HUANGQI and its potential targets and mechanisms of action in AD.Materials and methods The active ingredients and targets of HUANGQI were screened from databases (TCSMP, ETCM, and BATMan), and AD-related genes were obtained from DrugBank and GeneCards. The same target genes were screened, and a drug-target disease network was constructed. The PPI network was constructed and GO and KEGG pathway enrichment analyses of the targets. The Cell Counting Kit-8 (CCK-8) assay was used to determine suitable HUANGQI treatment concentrations for HT-22 cells between 0-480 μg/mL. CCK-8, FITC-phalloidin and propidium iodide (PI) assays were used to examine the protective effect of (0, 60, 120, 240 μg/mL) of HUANGQI on 20 μM Aβ1-42-induced HT-22 cell cytotoxicity.Results Twelve active ingredients of HUANGQI were selected, with 679 common targets associated with AD. GO and KEGG analysis revealed that the therapeutic mechanisms of HUANGQI involve TNF, AGE, the NF-κB pathway, and nuclear receptor activity-related processes. The CCK-8 assay indicated that HUANGQI was not cytotoxic to HT-22 cells at concentrations less than 240 μg/mL and was able to attenuate Aβ1-42-induced cellular damage (EC50 = 83.46 μg/mL). FITC-phalloidin and PI assays suggested that HUANGQI could alleviate 20 μM Aβ1-42-induced neuronal cell cytotoxicity in a dose-dependent manner.Conclusion HUANGQI has a protective effect on Aβ1-42-induced nerve cell injury; further mechanism research was needed.https://www.tandfonline.com/doi/10.1080/13880209.2024.2382962Anti-inflammatoryantioxidantmolecular simulation |
| spellingShingle | Feng Lv Mei Sun Chunmeng Qin Dan Du Xiangru Zheng Wenjun Li Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology Pharmaceutical Biology Anti-inflammatory antioxidant molecular simulation |
| title | Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology |
| title_full | Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology |
| title_fullStr | Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology |
| title_full_unstemmed | Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology |
| title_short | Study of the multitarget mechanism of Astragalus (HUANGQI) in the treatment of Alzheimer’s disease based on network pharmacology and molecular docking technology |
| title_sort | study of the multitarget mechanism of astragalus huangqi in the treatment of alzheimer s disease based on network pharmacology and molecular docking technology |
| topic | Anti-inflammatory antioxidant molecular simulation |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2024.2382962 |
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