Development of a quantitative PMA-16S rRNA gene sequencing workflow for absolute abundance measurements of seawater microbial communities

Development of a quantitative PMA-16S rRNA gene sequencing workflow for absolute abundance measurements of seawater microbial communities

Abstract Background Ecological risk assessments rarely consider the impacts of environmental stress on microbial communities. Incorporating microbial community responses into these evaluations requires establishing sensitivity thresholds based on the absolute abundance of viable taxa. While essentia...

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Bibliographic Details
Main Authors: Marie C. Thomas, Gretel Waugh, Katarina Damjanovic, Inka Vanwonterghem, Nicole S. Webster, Andrew P. Negri, Heidi M. Luter
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Environmental Microbiome
Subjects:
Microbial ecotoxicology
Marine Microbiome
Response modelling
Cell viability
PMA
Absolute quantification
Online Access:https://doi.org/10.1186/s40793-025-00741-2
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