The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration
This study aims to determine the effect of nanoparticles (NPs), namely graphene oxide (GO), multilayer carbon tubes (MWCNTs + Fe), magnetite (Fe3O4) and in situ forming brushite (DCPD) in chitosan (CS) based matrices on the cytotoxicity, Pregabalin (PG) release, and in vivo behavior of mechanically...
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| Format: | Article |
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Elsevier
2024-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893924001087 |
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| author | Liudmyla Sukhodub Mariia Kumeda Leonid Sukhodub Oleksandr Tsyndrenko Oleksandr Petrenko Volodymyr Prokopiuk Anton Tkachenko |
| author_facet | Liudmyla Sukhodub Mariia Kumeda Leonid Sukhodub Oleksandr Tsyndrenko Oleksandr Petrenko Volodymyr Prokopiuk Anton Tkachenko |
| author_sort | Liudmyla Sukhodub |
| collection | DOAJ |
| description | This study aims to determine the effect of nanoparticles (NPs), namely graphene oxide (GO), multilayer carbon tubes (MWCNTs + Fe), magnetite (Fe3O4) and in situ forming brushite (DCPD) in chitosan (CS) based matrices on the cytotoxicity, Pregabalin (PG) release, and in vivo behavior of mechanically stabilized conductive nerve conduits (NCs). NPs change the composites’ hydrophilicity in the order: DCPD (46.8) < MWCNT (54.21) ∼ Fe3O4 (54.44) < GO (65.99) and the water contact angle (47–66°) corresponds to the conditions of cell adhesion. NPs of ≤ 150 μg/ml reduce cell adhesion but don't trigger oxidative stress and toxic effects. Magnetite NPs of 300 µg/ml promote apoptosis in nerve cells. The introduction of PG at stage b during the DCPD formation provides the drug release kinetics closest to the zero-order kinetic model. The most electrically conductive GO- and MWCNTs + Fe - containing NCs have a prolonged biodegradation period of about 6 months, which makes them promising for the regeneration of damaged peripheral nerves in humans. |
| format | Article |
| id | doaj-art-9d924fb8b1b94f93ae5ffaa72b2490d3 |
| institution | Kabale University |
| issn | 2666-8939 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-9d924fb8b1b94f93ae5ffaa72b2490d32024-12-13T11:06:16ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392024-12-018100528The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regenerationLiudmyla Sukhodub0Mariia Kumeda1Leonid Sukhodub2Oleksandr Tsyndrenko3Oleksandr Petrenko4Volodymyr Prokopiuk5Anton Tkachenko6Sumy State University, Sumy, 40007, Ukraine; Corresponding author.Sumy State University, Sumy, 40007, UkraineSumy State University, Sumy, 40007, UkraineSumy State University, Sumy, 40007, UkraineDepartment of Cryobiochemistry, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, UkraineResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, 61022, Ukraine; Department of Cryobiochemistry, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, UkraineDepartment of Cryobiochemistry, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, UkraineThis study aims to determine the effect of nanoparticles (NPs), namely graphene oxide (GO), multilayer carbon tubes (MWCNTs + Fe), magnetite (Fe3O4) and in situ forming brushite (DCPD) in chitosan (CS) based matrices on the cytotoxicity, Pregabalin (PG) release, and in vivo behavior of mechanically stabilized conductive nerve conduits (NCs). NPs change the composites’ hydrophilicity in the order: DCPD (46.8) < MWCNT (54.21) ∼ Fe3O4 (54.44) < GO (65.99) and the water contact angle (47–66°) corresponds to the conditions of cell adhesion. NPs of ≤ 150 μg/ml reduce cell adhesion but don't trigger oxidative stress and toxic effects. Magnetite NPs of 300 µg/ml promote apoptosis in nerve cells. The introduction of PG at stage b during the DCPD formation provides the drug release kinetics closest to the zero-order kinetic model. The most electrically conductive GO- and MWCNTs + Fe - containing NCs have a prolonged biodegradation period of about 6 months, which makes them promising for the regeneration of damaged peripheral nerves in humans.http://www.sciencedirect.com/science/article/pii/S2666893924001087ChitosanCarbon nanoparticlesCytotoxicityDrug releaseNerve conduitIn vivo biocompatibility |
| spellingShingle | Liudmyla Sukhodub Mariia Kumeda Leonid Sukhodub Oleksandr Tsyndrenko Oleksandr Petrenko Volodymyr Prokopiuk Anton Tkachenko The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration Carbohydrate Polymer Technologies and Applications Chitosan Carbon nanoparticles Cytotoxicity Drug release Nerve conduit In vivo biocompatibility |
| title | The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration |
| title_full | The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration |
| title_fullStr | The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration |
| title_full_unstemmed | The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration |
| title_short | The effect of carbon and magnetic nanoparticles on the properties of chitosan-based neural tubes: Cytotoxicity, drug release, In Vivo nerve regeneration |
| title_sort | effect of carbon and magnetic nanoparticles on the properties of chitosan based neural tubes cytotoxicity drug release in vivo nerve regeneration |
| topic | Chitosan Carbon nanoparticles Cytotoxicity Drug release Nerve conduit In vivo biocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S2666893924001087 |
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