Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production
This study examined the optimization of growth conditions of Serratia marcescens isolated from a loamy soil of the Federal University Technology Minna Nigeria, for prodigiosin production. The different optimized conditions examined were extraction solvents, incubation time, pH, temperature, carbon...
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Umaru Musa Yar'adua University, Katsina, Nigeria
2017-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/263 |
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| author | Shaba, A.M. Oyeleke, S.B. Ijah U.J.J. Oyewole, O.A. Adamu, B.B. Okeke, K.S. Baba, J. Cletus C.I. |
| author_facet | Shaba, A.M. Oyeleke, S.B. Ijah U.J.J. Oyewole, O.A. Adamu, B.B. Okeke, K.S. Baba, J. Cletus C.I. |
| author_sort | Shaba, A.M. |
| collection | DOAJ |
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This study examined the optimization of growth conditions of Serratia marcescens isolated from a loamy soil of the Federal University Technology Minna Nigeria, for prodigiosin production. The different optimized conditions examined were extraction solvents, incubation time, pH, temperature, carbon sources, organic nitrogen sources, inorganic nitrogen sources and agitation levels. The result reveals that methanol is an ideal solvent recording absorbance of 0.69nm, which is slightly followed by ethanol and acetone with absorbance of 0.4nm and 0.3nm respectively. The bacterium produced maximum level of pigment after 36 hours of incubation (22.20µg/L), although pigment production was observed from 12 hours of incubation onwards (7.40µg/L). The pigment production declined after 36 hours of incubation. The results also revealed that pigment production by S. marcescens was maximum at 25°C (25.10µg/L) followed by 30oC (22.50µg/L). The optimal production was obtained at pH 7 (25.00mg/ml) with dextrose as optimal source of carbon (22.40mg/ml). There was no pigment production at static condition but maximum pigment production was recorded at 150 rpm (22.50µg/L). All the inorganic nitrogen sources caused a reduced biomass production. Among the organic nitrogen sources tested, yeast extract supported maximum pigment (26.75µg/L) but peptone led to a decreased pigment production (9.15µg/L) compared to other organic sources. Addition of urea did not support pigment as well as biomass production. Statistical analysis shows significant differences (p<0.05) in prodigiosin productions with different substrates, temperature and agitation levels. The study revealed that the production of prodigiosin was significantly influenced by the extraction solvents, incubation time, pH, temperature, carbon sources, organic nitrogen sources, inorganic nitrogen sources and agitation levels.
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| format | Article |
| id | doaj-art-9d529a723c4c4a0d865dba7b49b02d97 |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-9d529a723c4c4a0d865dba7b49b02d972024-11-28T11:09:13ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222017-12-012210.47430/ujmr.1722.005263Optimization of Growth Conditions of Serratia marcescens for Prodigiosin ProductionShaba, A.M.0Oyeleke, S.B.1Ijah U.J.J.2Oyewole, O.A.3Adamu, B.B.4Okeke, K.S.5Baba, J.6Cletus C.I.7Department of Biological Sciences, Niger State Polytechnic Zungeru, NigeriaDepartment of Microbiology, Federal University of Technology, Minna, NigeriaDepartment of Microbiology, Federal University of Technology, Minna, NigeriaDepartment of Microbiology, Federal University of Technology, Minna, NigeriaDepartment of Microbiology, Federal University of Technology, Minna, Nigeria Department of Nutrition and Dietetics, Federal Polytechnic Bida, Nigeria Department of Microbiology, Ibrahim Badamosi Babangida University, Lapai, NigeriaDepartment of Microbiology, Federal University of Technology, Minna, Nigeria This study examined the optimization of growth conditions of Serratia marcescens isolated from a loamy soil of the Federal University Technology Minna Nigeria, for prodigiosin production. The different optimized conditions examined were extraction solvents, incubation time, pH, temperature, carbon sources, organic nitrogen sources, inorganic nitrogen sources and agitation levels. The result reveals that methanol is an ideal solvent recording absorbance of 0.69nm, which is slightly followed by ethanol and acetone with absorbance of 0.4nm and 0.3nm respectively. The bacterium produced maximum level of pigment after 36 hours of incubation (22.20µg/L), although pigment production was observed from 12 hours of incubation onwards (7.40µg/L). The pigment production declined after 36 hours of incubation. The results also revealed that pigment production by S. marcescens was maximum at 25°C (25.10µg/L) followed by 30oC (22.50µg/L). The optimal production was obtained at pH 7 (25.00mg/ml) with dextrose as optimal source of carbon (22.40mg/ml). There was no pigment production at static condition but maximum pigment production was recorded at 150 rpm (22.50µg/L). All the inorganic nitrogen sources caused a reduced biomass production. Among the organic nitrogen sources tested, yeast extract supported maximum pigment (26.75µg/L) but peptone led to a decreased pigment production (9.15µg/L) compared to other organic sources. Addition of urea did not support pigment as well as biomass production. Statistical analysis shows significant differences (p<0.05) in prodigiosin productions with different substrates, temperature and agitation levels. The study revealed that the production of prodigiosin was significantly influenced by the extraction solvents, incubation time, pH, temperature, carbon sources, organic nitrogen sources, inorganic nitrogen sources and agitation levels. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/263Prodigiosinsolventssubstratesagitationincubation conditionsSerratia marcescens |
| spellingShingle | Shaba, A.M. Oyeleke, S.B. Ijah U.J.J. Oyewole, O.A. Adamu, B.B. Okeke, K.S. Baba, J. Cletus C.I. Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production UMYU Journal of Microbiology Research Prodigiosin solvents substrates agitation incubation conditions Serratia marcescens |
| title | Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production |
| title_full | Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production |
| title_fullStr | Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production |
| title_full_unstemmed | Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production |
| title_short | Optimization of Growth Conditions of Serratia marcescens for Prodigiosin Production |
| title_sort | optimization of growth conditions of serratia marcescens for prodigiosin production |
| topic | Prodigiosin solvents substrates agitation incubation conditions Serratia marcescens |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/263 |
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