Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
<b>Aim:</b> The purpose of this study was to investigate the plasmid profiles of Enterococcus faecalis isolated from cow milk samples with mastitis.<p> <b>Materials and Methods:</b> As a material, 54 E. faecalis strains isolated in the past years from milk samples with...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1156 |
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| Summary: | <b>Aim:</b> The purpose of this study was to investigate the plasmid
profiles of Enterococcus faecalis isolated from cow milk
samples with mastitis.<p>
<b>Materials and Methods:</b> As a material, 54 E. faecalis strains
isolated in the past years from milk samples with mastitis
were used. Modified alkaline lysis method was used for plasmid
isolation from bacteria.<p>
<b>Results:</b> Results revealed that 19 of isolates have plasmids
varying one to eight and lengths of those were ranged approximately
between 0.6 kb and 41 kb. It was determined
that five isolates had only one plasmid while the other 14
had more plasmids. Following the plasmid isolation, cluster
analysis was performed between the same and similar plasmids
after their profile contends checked. Six clusters that
containing two or more strains with similar plasmid profile
were formed.<p>
<b>Conclusions:</b> Further studies should be designed to investigate
the plasmids of other enterococci stains isolated from
mastitis cow’s milks whether plasmids are responsible for
antibiotic resistance. |
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| ISSN: | 1309-6958 2146-1953 |