Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage
【Objective】The physiological and biochemical mechanisms of Canna generalis Bailey seedlings in response to nitrate stress were studied in order to provide a basis for improving water eutrophication by planting Canna generalis Bailey.【Method】Using Yunnan Canna generalis bailey as the material, hydrop...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Guangdong Academy of Agricultural Sciences
2024-10-01
|
| Series: | Guangdong nongye kexue |
| Subjects: | |
| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202410005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841560353382596608 |
|---|---|
| author | Zhaohu GU Shuxin ZHAO Wenwei WU Huini XU Kunzhi LI |
| author_facet | Zhaohu GU Shuxin ZHAO Wenwei WU Huini XU Kunzhi LI |
| author_sort | Zhaohu GU |
| collection | DOAJ |
| description | 【Objective】The physiological and biochemical mechanisms of Canna generalis Bailey seedlings in response to nitrate stress were studied in order to provide a basis for improving water eutrophication by planting Canna generalis Bailey.【Method】Using Yunnan Canna generalis bailey as the material, hydroponic methods were used to study the nitrogen uptake, seedling growth, photosynthetic parameters, antioxidant enzyme activity as well as physiological and biochemical indexes of Canna generalis bailey under different nitrate nitrogen treatments.【Result】After treatment with 0 (CK), 10, 50, 100 and 150 mmol/L nitrate solutions for 5 weeks, the total nitrate uptake amounts of Canna generalis Bailey was 0, 411.7, 676.9, 554.6 and 516.4 mg/plant, the plant height increased by -0.27, 5.25, 4.25, 0.05 and -10.32 cm, and the fresh weight increased by 10.3, 32.7, 31.6, 20.3 and -11.0 g/plant, respectively. Within 5 weeks of hydroponic treatment, the photosynthesis and transpiration rates of 10 mmol/L and 50 mmol/L low concentration nitrate were significantly higher than those of the control. In the fifth week, the photosynthesis rate was about 11 and 15 times that of CK, and the transpiration rate was 4.3 and 7.5 times that of CK. Stomatal conductance and intercellular CO2 concentration remained high. The photosynthesis and transpiration rates of plants treated with 100 and 150 mmol/L nitrate solutions were at a low level, and the stomatal conductance was extremely low. The activities of SOD and CAT under 0 and 10 mmol/L treatments were relatively low, while those under 50, 100 and 150 mmol/L treatments were significantly higher than those in the control. The activities of SOD and CAT under 50 mmol/L treatment were the highest and significantly higher than those under other treatments. POD activity was significantly higher at 50 mmol/L than that of other treatments within 5 weeks, and the lowest at 150 mmol/L, indicating that high concentration of NO3- inhibited POD enzyme activity.【Conclusion】A lower concentration of 50 mmol/L nitrate can enhance the photosynthesis, antioxidant enzyme activity, and nitrate nitrogen absorption of Canna generalis Bailey, promoting its growth. However, a higher concentration of 100 mmol/L nitrate reduces the photosynthesis, antioxidant enzyme activity, and nitrate nitrogen absorption of Canna generalis Bailey, inhibiting its growth. |
| format | Article |
| id | doaj-art-9cdf5f3cfc8c4c31b6dcad3dcdc813e0 |
| institution | Kabale University |
| issn | 1004-874X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
| record_format | Article |
| series | Guangdong nongye kexue |
| spelling | doaj-art-9cdf5f3cfc8c4c31b6dcad3dcdc813e02025-01-04T07:39:37ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2024-10-015110536110.16768/j.issn.1004-874X.2024.10.005202410005Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling StageZhaohu GU0Shuxin ZHAO1Wenwei WU2Huini XU3Kunzhi LI4Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, ChinaYunnan Academy of Environmental Sciences, Kunming 650034, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China【Objective】The physiological and biochemical mechanisms of Canna generalis Bailey seedlings in response to nitrate stress were studied in order to provide a basis for improving water eutrophication by planting Canna generalis Bailey.【Method】Using Yunnan Canna generalis bailey as the material, hydroponic methods were used to study the nitrogen uptake, seedling growth, photosynthetic parameters, antioxidant enzyme activity as well as physiological and biochemical indexes of Canna generalis bailey under different nitrate nitrogen treatments.【Result】After treatment with 0 (CK), 10, 50, 100 and 150 mmol/L nitrate solutions for 5 weeks, the total nitrate uptake amounts of Canna generalis Bailey was 0, 411.7, 676.9, 554.6 and 516.4 mg/plant, the plant height increased by -0.27, 5.25, 4.25, 0.05 and -10.32 cm, and the fresh weight increased by 10.3, 32.7, 31.6, 20.3 and -11.0 g/plant, respectively. Within 5 weeks of hydroponic treatment, the photosynthesis and transpiration rates of 10 mmol/L and 50 mmol/L low concentration nitrate were significantly higher than those of the control. In the fifth week, the photosynthesis rate was about 11 and 15 times that of CK, and the transpiration rate was 4.3 and 7.5 times that of CK. Stomatal conductance and intercellular CO2 concentration remained high. The photosynthesis and transpiration rates of plants treated with 100 and 150 mmol/L nitrate solutions were at a low level, and the stomatal conductance was extremely low. The activities of SOD and CAT under 0 and 10 mmol/L treatments were relatively low, while those under 50, 100 and 150 mmol/L treatments were significantly higher than those in the control. The activities of SOD and CAT under 50 mmol/L treatment were the highest and significantly higher than those under other treatments. POD activity was significantly higher at 50 mmol/L than that of other treatments within 5 weeks, and the lowest at 150 mmol/L, indicating that high concentration of NO3- inhibited POD enzyme activity.【Conclusion】A lower concentration of 50 mmol/L nitrate can enhance the photosynthesis, antioxidant enzyme activity, and nitrate nitrogen absorption of Canna generalis Bailey, promoting its growth. However, a higher concentration of 100 mmol/L nitrate reduces the photosynthesis, antioxidant enzyme activity, and nitrate nitrogen absorption of Canna generalis Bailey, inhibiting its growth.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202410005canna generalis baileynitrate nitrogenphotosynthesisantioxidant enzymephysiological indextranspiration rate |
| spellingShingle | Zhaohu GU Shuxin ZHAO Wenwei WU Huini XU Kunzhi LI Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage Guangdong nongye kexue canna generalis bailey nitrate nitrogen photosynthesis antioxidant enzyme physiological index transpiration rate |
| title | Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage |
| title_full | Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage |
| title_fullStr | Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage |
| title_full_unstemmed | Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage |
| title_short | Effects of Nitrate Stress on Physiological and Biochemical Characteristics of Canna generalis Bailey at Seedling Stage |
| title_sort | effects of nitrate stress on physiological and biochemical characteristics of canna generalis bailey at seedling stage |
| topic | canna generalis bailey nitrate nitrogen photosynthesis antioxidant enzyme physiological index transpiration rate |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202410005 |
| work_keys_str_mv | AT zhaohugu effectsofnitratestressonphysiologicalandbiochemicalcharacteristicsofcannageneralisbaileyatseedlingstage AT shuxinzhao effectsofnitratestressonphysiologicalandbiochemicalcharacteristicsofcannageneralisbaileyatseedlingstage AT wenweiwu effectsofnitratestressonphysiologicalandbiochemicalcharacteristicsofcannageneralisbaileyatseedlingstage AT huinixu effectsofnitratestressonphysiologicalandbiochemicalcharacteristicsofcannageneralisbaileyatseedlingstage AT kunzhili effectsofnitratestressonphysiologicalandbiochemicalcharacteristicsofcannageneralisbaileyatseedlingstage |