Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization
BackgroundPolydatin (PD), also known as tiger cane glycoside, is a natural compound extracted from the Japanese knotweed plant, which is often referred to as white resveratrol. It exhibits anti-inflammatory, antioxidant, and anti-apoptotic effects in the treatment of various diseases. However, the p...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1514483/full |
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| author | Qi Sun Xin-Yu Nan Hui Wang Shuo Pan Gang Ji Ya-Feng Guo Ya-Heng Zhao Gao-Cen Li Shao-Shi Guo Lu-Feng Lin Yu-Jie Jin Xue Li Zhang Chang-Cheng Liu Guo-Bin Liu |
| author_facet | Qi Sun Xin-Yu Nan Hui Wang Shuo Pan Gang Ji Ya-Feng Guo Ya-Heng Zhao Gao-Cen Li Shao-Shi Guo Lu-Feng Lin Yu-Jie Jin Xue Li Zhang Chang-Cheng Liu Guo-Bin Liu |
| author_sort | Qi Sun |
| collection | DOAJ |
| description | BackgroundPolydatin (PD), also known as tiger cane glycoside, is a natural compound extracted from the Japanese knotweed plant, which is often referred to as white resveratrol. It exhibits anti-inflammatory, antioxidant, and anti-apoptotic effects in the treatment of various diseases. However, the potential molecular mechanisms of PD in osteoarthritis have not been clearly elucidated.MethodsAnterior cruciate ligament transection (ACLT) surgery was performed to establish an osteoarthritis animal model. Female mice at the age of 12 weeks were intraperitoneally injected with different concentrations of PD (20 and 40 mg/kg). In vitro models were established by isolating mouse articular chondrocytes, which were subsequently treated with lipopolysaccharide or IL-1β for 24 h for subsequent experiments. In addition, different concentrations of PD were administered for 12 h. Morphological changes were observed by toluidine blue staining, joint bone metabolism changes were observed by tartrate-resistant acid phosphatase staining, immunohistochemistry was used to observe the expression levels of inflammatory factors and extracellular matrix. MicroCT analysis was conducted to assess changes in the microstructure of subchondral bone trabeculae, and Western blot was performed to measure the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway and markers of M1 polarization in macrophages.ResultsPD significantly delays the progression of osteoarthritis induced by ACLT, effectively inhibits IL-1β-induced joint inflammation, bone metabolic remodeling and extracellular matrix degradation. In addition, paeoniflorin markedly suppresses the transmission of the NF-κB signaling pathway and reverses M1 polarization in macrophages induced by IL-1β.ConclusionTaken together, PD might be a potential therapeutic agent for the prevention and treatment of osteoarthritis. |
| format | Article |
| id | doaj-art-9cab0d45c40047cd85d03c2d8b37747b |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-9cab0d45c40047cd85d03c2d8b37747b2025-01-07T06:48:07ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15144831514483Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarizationQi Sun0Xin-Yu Nan1Hui Wang2Shuo Pan3Gang Ji4Ya-Feng Guo5Ya-Heng Zhao6Gao-Cen Li7Shao-Shi Guo8Lu-Feng Lin9Yu-Jie Jin10Xue Li Zhang11Chang-Cheng Liu12Guo-Bin Liu13The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaHebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe First Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaBackgroundPolydatin (PD), also known as tiger cane glycoside, is a natural compound extracted from the Japanese knotweed plant, which is often referred to as white resveratrol. It exhibits anti-inflammatory, antioxidant, and anti-apoptotic effects in the treatment of various diseases. However, the potential molecular mechanisms of PD in osteoarthritis have not been clearly elucidated.MethodsAnterior cruciate ligament transection (ACLT) surgery was performed to establish an osteoarthritis animal model. Female mice at the age of 12 weeks were intraperitoneally injected with different concentrations of PD (20 and 40 mg/kg). In vitro models were established by isolating mouse articular chondrocytes, which were subsequently treated with lipopolysaccharide or IL-1β for 24 h for subsequent experiments. In addition, different concentrations of PD were administered for 12 h. Morphological changes were observed by toluidine blue staining, joint bone metabolism changes were observed by tartrate-resistant acid phosphatase staining, immunohistochemistry was used to observe the expression levels of inflammatory factors and extracellular matrix. MicroCT analysis was conducted to assess changes in the microstructure of subchondral bone trabeculae, and Western blot was performed to measure the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway and markers of M1 polarization in macrophages.ResultsPD significantly delays the progression of osteoarthritis induced by ACLT, effectively inhibits IL-1β-induced joint inflammation, bone metabolic remodeling and extracellular matrix degradation. In addition, paeoniflorin markedly suppresses the transmission of the NF-κB signaling pathway and reverses M1 polarization in macrophages induced by IL-1β.ConclusionTaken together, PD might be a potential therapeutic agent for the prevention and treatment of osteoarthritis.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1514483/fullPolydatincartilageextracellular matrixNF-κBosteoarthritis |
| spellingShingle | Qi Sun Xin-Yu Nan Hui Wang Shuo Pan Gang Ji Ya-Feng Guo Ya-Heng Zhao Gao-Cen Li Shao-Shi Guo Lu-Feng Lin Yu-Jie Jin Xue Li Zhang Chang-Cheng Liu Guo-Bin Liu Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization Frontiers in Bioengineering and Biotechnology Polydatin cartilage extracellular matrix NF-κB osteoarthritis |
| title | Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization |
| title_full | Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization |
| title_fullStr | Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization |
| title_full_unstemmed | Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization |
| title_short | Polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization |
| title_sort | polydatin retards the progression of osteoarthritis by maintaining bone metabolicbalance and inhibiting macrophage polarization |
| topic | Polydatin cartilage extracellular matrix NF-κB osteoarthritis |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1514483/full |
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