Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment
Lignin is known to limit the enzyme-mediated hydrolysis of biomass by both restricting substrate swelling and binding to the enzymes. Pretreated mechanical pulp (MP) made from Aspen wood chips was incubated with either 16% sodium sulfite or 32% sodium percarbonate to incorporate similar amounts of s...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-11-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2020.608835/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556110226489344 |
|---|---|
| author | Jie Wu Richard P. Chandra Masatsugu Takada Masatsugu Takada Li-Yang Liu Scott Renneckar Kwang Ho Kim Chang Soo Kim Jack N. Saddler |
| author_facet | Jie Wu Richard P. Chandra Masatsugu Takada Masatsugu Takada Li-Yang Liu Scott Renneckar Kwang Ho Kim Chang Soo Kim Jack N. Saddler |
| author_sort | Jie Wu |
| collection | DOAJ |
| description | Lignin is known to limit the enzyme-mediated hydrolysis of biomass by both restricting substrate swelling and binding to the enzymes. Pretreated mechanical pulp (MP) made from Aspen wood chips was incubated with either 16% sodium sulfite or 32% sodium percarbonate to incorporate similar amounts of sulfonic and carboxylic acid groups onto the lignin (60 mmol/kg substrate) present in the pulp without resulting in significant delignification. When Simon’s stain was used to assess potential enzyme accessibility to the cellulose, it was apparent that both post-treatments enhanced accessibility and cellulose hydrolysis. To further elucidate how acid group addition might influence potential enzyme binding to lignin, Protease Treated Lignin (PTL) was isolated from the original and modified mechanical pulps and added to a cellulose rich, delignified Kraft pulp. As anticipated, the PTLs from both the oxidized and sulfonated substrates proved less inhibitory and adsorbed less enzymes than did the PTL derived from the original pulp. Subsequent analyses indicated that both the sulfonated and oxidized lignin samples contained less phenolic hydroxyl groups, resulting in enhanced hydrophilicity and a more negative charge which decreased the non-productive binding of the cellulase enzymes to the lignin. |
| format | Article |
| id | doaj-art-9c765a2d31d24162bd7f6ab5b038eb5c |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-9c765a2d31d24162bd7f6ab5b038eb5c2025-01-07T13:52:49ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-11-01810.3389/fbioe.2020.608835608835Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass PretreatmentJie Wu0Richard P. Chandra1Masatsugu Takada2Masatsugu Takada3Li-Yang Liu4Scott Renneckar5Kwang Ho Kim6Chang Soo Kim7Jack N. Saddler8Forest Product Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaForest Product Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaForest Product Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaInternational Advanced Energy Science Research and Education Center, Graduate School of Energy Science, Kyoto University, Kyoto, JapanAdvanced Renewable Materials Lab, Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaAdvanced Renewable Materials Lab, Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaClean Energy Research Center, Korea Institute of Science and Technology, Seoul, South KoreaClean Energy Research Center, Korea Institute of Science and Technology, Seoul, South KoreaForest Product Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, University of British Columbia, Vancouver, BC, CanadaLignin is known to limit the enzyme-mediated hydrolysis of biomass by both restricting substrate swelling and binding to the enzymes. Pretreated mechanical pulp (MP) made from Aspen wood chips was incubated with either 16% sodium sulfite or 32% sodium percarbonate to incorporate similar amounts of sulfonic and carboxylic acid groups onto the lignin (60 mmol/kg substrate) present in the pulp without resulting in significant delignification. When Simon’s stain was used to assess potential enzyme accessibility to the cellulose, it was apparent that both post-treatments enhanced accessibility and cellulose hydrolysis. To further elucidate how acid group addition might influence potential enzyme binding to lignin, Protease Treated Lignin (PTL) was isolated from the original and modified mechanical pulps and added to a cellulose rich, delignified Kraft pulp. As anticipated, the PTLs from both the oxidized and sulfonated substrates proved less inhibitory and adsorbed less enzymes than did the PTL derived from the original pulp. Subsequent analyses indicated that both the sulfonated and oxidized lignin samples contained less phenolic hydroxyl groups, resulting in enhanced hydrophilicity and a more negative charge which decreased the non-productive binding of the cellulase enzymes to the lignin.https://www.frontiersin.org/articles/10.3389/fbioe.2020.608835/fullligninoxidationsulfonationcellulase enzymesnon-productive bindingpH |
| spellingShingle | Jie Wu Richard P. Chandra Masatsugu Takada Masatsugu Takada Li-Yang Liu Scott Renneckar Kwang Ho Kim Chang Soo Kim Jack N. Saddler Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment Frontiers in Bioengineering and Biotechnology lignin oxidation sulfonation cellulase enzymes non-productive binding pH |
| title | Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment |
| title_full | Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment |
| title_fullStr | Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment |
| title_full_unstemmed | Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment |
| title_short | Enhancing Enzyme-Mediated Cellulose Hydrolysis by Incorporating Acid Groups Onto the Lignin During Biomass Pretreatment |
| title_sort | enhancing enzyme mediated cellulose hydrolysis by incorporating acid groups onto the lignin during biomass pretreatment |
| topic | lignin oxidation sulfonation cellulase enzymes non-productive binding pH |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2020.608835/full |
| work_keys_str_mv | AT jiewu enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT richardpchandra enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT masatsugutakada enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT masatsugutakada enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT liyangliu enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT scottrenneckar enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT kwanghokim enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT changsookim enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment AT jacknsaddler enhancingenzymemediatedcellulosehydrolysisbyincorporatingacidgroupsontotheligninduringbiomasspretreatment |