Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles
Abstract Structurally defined, protein‐grafted nanoparticles are widely used in various biomedical applications, particularly as intelligent nanocarriers for drug delivery. The integration of synthetic polymers with natural proteins such as silk sericin enhances the functionality and stability of th...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2024-12-01
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| Series: | Macromolecular Materials and Engineering |
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| Online Access: | https://doi.org/10.1002/mame.202400158 |
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| author | Ionut‐Cristian Radu Derniza‐Elena Cozorici Erika Blanzeanu Andreea Vadureanu Cristina Stavarache Eugenia Tanasa Horia Iovu Catalin Zaharia |
| author_facet | Ionut‐Cristian Radu Derniza‐Elena Cozorici Erika Blanzeanu Andreea Vadureanu Cristina Stavarache Eugenia Tanasa Horia Iovu Catalin Zaharia |
| author_sort | Ionut‐Cristian Radu |
| collection | DOAJ |
| description | Abstract Structurally defined, protein‐grafted nanoparticles are widely used in various biomedical applications, particularly as intelligent nanocarriers for drug delivery. The integration of synthetic polymers with natural proteins such as silk sericin enhances the functionality and stability of these nanocarriers, making them suitable for targeted and controlled drug release. In this context, an optimized grafting procedure for silk sericin is presented, employing a protein macroinitiator and atom transfer radical polymerization (ATRP). This study aims to elucidate the significance of the grafting process in tailoring the structure of sericin through the chemistry of synthetic grafts. The grafting procedure uses block copolymers of N‐isopropylacrylamide (NIPAM) and 2‐acrylamido‐2‐methylpropanesulfonic acid (AMPS), such as Poly‐(AMPS‐block‐NIPAM)/Poly‐(NIPAM‐block‐AMPS). The procedure employs both one‐step and two‐step synthesis methods to produce a well‐defined, biofunctionalized sericin. Subsequently, sericin‐based nanoparticles are prepared, demonstrating the significance of the optimized procedure. The synthesized products undergo structural analysis using H‐NMR, FTIR‐ATR, XPS, DLS, and zeta potential measurements. In addition, their thermal behavior is assessed using differential scanning calorimetry. To further investigate the prepared nanoparticles, SEM and DLS analyses are conducted. Through synthesis optimization, position and length of each synthetic block is precisely determined, significantly influencing properties of the grafted products and characteristics of the resulting nanoparticles. |
| format | Article |
| id | doaj-art-9c4078e1b3b84520b0a49ad2d5925a4f |
| institution | Kabale University |
| issn | 1438-7492 1439-2054 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj-art-9c4078e1b3b84520b0a49ad2d5925a4f2024-12-17T01:22:03ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542024-12-0130912n/an/a10.1002/mame.202400158Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block NanoparticlesIonut‐Cristian Radu0Derniza‐Elena Cozorici1Erika Blanzeanu2Andreea Vadureanu3Cristina Stavarache4Eugenia Tanasa5Horia Iovu6Catalin Zaharia7Advanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAdvanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAdvanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAdvanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAdvanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaDepartment of Physics Faculty of Applied Sciences National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAdvanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAdvanced Polymer Materials Group Faculty of Chemical Engineering and Biotechnology Department of Bioresources and Polymer Science National University of Science and Technology POLITEHNICA Bucharest Bucharest 011061 RomaniaAbstract Structurally defined, protein‐grafted nanoparticles are widely used in various biomedical applications, particularly as intelligent nanocarriers for drug delivery. The integration of synthetic polymers with natural proteins such as silk sericin enhances the functionality and stability of these nanocarriers, making them suitable for targeted and controlled drug release. In this context, an optimized grafting procedure for silk sericin is presented, employing a protein macroinitiator and atom transfer radical polymerization (ATRP). This study aims to elucidate the significance of the grafting process in tailoring the structure of sericin through the chemistry of synthetic grafts. The grafting procedure uses block copolymers of N‐isopropylacrylamide (NIPAM) and 2‐acrylamido‐2‐methylpropanesulfonic acid (AMPS), such as Poly‐(AMPS‐block‐NIPAM)/Poly‐(NIPAM‐block‐AMPS). The procedure employs both one‐step and two‐step synthesis methods to produce a well‐defined, biofunctionalized sericin. Subsequently, sericin‐based nanoparticles are prepared, demonstrating the significance of the optimized procedure. The synthesized products undergo structural analysis using H‐NMR, FTIR‐ATR, XPS, DLS, and zeta potential measurements. In addition, their thermal behavior is assessed using differential scanning calorimetry. To further investigate the prepared nanoparticles, SEM and DLS analyses are conducted. Through synthesis optimization, position and length of each synthetic block is precisely determined, significantly influencing properties of the grafted products and characteristics of the resulting nanoparticles.https://doi.org/10.1002/mame.202400158ATRPPNIPAM/PAMPSprotein macroinitiatorsilk sericinsynthetic grafts |
| spellingShingle | Ionut‐Cristian Radu Derniza‐Elena Cozorici Erika Blanzeanu Andreea Vadureanu Cristina Stavarache Eugenia Tanasa Horia Iovu Catalin Zaharia Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles Macromolecular Materials and Engineering ATRP PNIPAM/PAMPS protein macroinitiator silk sericin synthetic grafts |
| title | Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles |
| title_full | Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles |
| title_fullStr | Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles |
| title_full_unstemmed | Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles |
| title_short | Tailoring Silk Sericin Grafting: Comparing One‐Step and Two‐Step Approaches for PNIPAM/PAMPS Block Nanoparticles |
| title_sort | tailoring silk sericin grafting comparing one step and two step approaches for pnipam pamps block nanoparticles |
| topic | ATRP PNIPAM/PAMPS protein macroinitiator silk sericin synthetic grafts |
| url | https://doi.org/10.1002/mame.202400158 |
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