Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival

Background SLE is associated with increased risk of diffuse large B-cell lymphoma (DLBCL). DLBCL is routinely classified by cell of origin (COO), with germinal centre B-cell (GCB) being more common and indicating better prognosis in the general population. We studied COO subtyping in patients with S...

Full description

Saved in:
Bibliographic Details
Main Authors: Rosalind Ramsey-Goldman, Diane L Kamen, Eva Baecklund, Basile Tessier-Cloutier, David DW Twa, Randy Gascoyne, Nathalie A Johnson, Carin Backlin, Ann E Clarke, Jennifer LF Lee, Pedro Farinha, Sasha Bernatsky
Format: Article
Language:English
Published: BMJ Publishing Group 2019-12-01
Series:Lupus Science and Medicine
Online Access:https://lupus.bmj.com/content/6/1/e000324.full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846121516641550336
author Rosalind Ramsey-Goldman
Diane L Kamen
Eva Baecklund
Basile Tessier-Cloutier
David DW Twa
Randy Gascoyne
Nathalie A Johnson
Carin Backlin
Ann E Clarke
Jennifer LF Lee
Pedro Farinha
Sasha Bernatsky
author_facet Rosalind Ramsey-Goldman
Diane L Kamen
Eva Baecklund
Basile Tessier-Cloutier
David DW Twa
Randy Gascoyne
Nathalie A Johnson
Carin Backlin
Ann E Clarke
Jennifer LF Lee
Pedro Farinha
Sasha Bernatsky
author_sort Rosalind Ramsey-Goldman
collection DOAJ
description Background SLE is associated with increased risk of diffuse large B-cell lymphoma (DLBCL). DLBCL is routinely classified by cell of origin (COO), with germinal centre B-cell (GCB) being more common and indicating better prognosis in the general population. We studied COO subtyping in patients with SLE diagnosed with DLBCL and their survival.Patients and methods We evaluated 20 cases of SLE with DLBCL. Immunohistochemistry analysis was performed (BCL2, MYC, BCL6, CD10, CD20, FOXP1, GCET1, MUM1) in tissue microarrays. We examined associations between molecular and clinical features, including overall survival.Results Of the 20 DLBCL SLE cases, 12/20 cases (60%) were classified as non-GCB using Hans or Choi algorithms. MYC and BCL2 protein expression was positive in 6/20 (30%) and 8/20 (40%) SLE cases, respectively, with 2/20 (10%) co-expressing both markers. Seven (7/20) had only extranodal involvement at DLBCL diagnosis. As expected, non-GCB cases had worse survival. Cases presenting exclusively with extranodal disease were associated with shorter SLE duration and better survival despite higher BCL2 protein expression.Conclusions We present novel data characterising DLBCL in SLE. Sixty per cent of the DLBCL in patients with SLE were non-GCB. The nodal and extranodal distribution in SLE was similar to what is known in the general population, but extranodal disease occurred more often with short SLE duration and was associated with longer overall survival. More research on cancer in SLE is the key to further understanding the complex interplay between cancer and the immune system.
format Article
id doaj-art-9c3e237f617f462eadca7a9b4ec1edfd
institution Kabale University
issn 2053-8790
language English
publishDate 2019-12-01
publisher BMJ Publishing Group
record_format Article
series Lupus Science and Medicine
spelling doaj-art-9c3e237f617f462eadca7a9b4ec1edfd2024-12-16T02:10:09ZengBMJ Publishing GroupLupus Science and Medicine2053-87902019-12-016110.1136/lupus-2019-000324Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survivalRosalind Ramsey-Goldman0Diane L Kamen1Eva Baecklund2Basile Tessier-Cloutier3David DW Twa4Randy Gascoyne5Nathalie A Johnson6Carin Backlin7Ann E Clarke8Jennifer LF Lee9Pedro Farinha10Sasha Bernatsky11Northwestern University Feinberg School of Medicine, Chicago, Illinois, USAMedical University of South Carolina, Charleston, South Carolina, USADepartment of Medical Sciences, Uppsala University, Uppsala, SwedenAnatomical Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, CanadaFaculty of Medicine, University of British Columbia, Vancouver, British Columbia, CanadaPathology Department and Centre for Lymphoid Cancer, British Columbia Cancer Agency, Vancouver, British Columbia, CanadaDepartment of Medicine, Sir Mortimer B Davis Jewish General Hospital, Montreal, Québec, CanadaDepartment of Rheumatology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USACumming School of Medicine, University of Calgary, Calgary, Alberta, CanadaDivision of Clinical Epidemiology, McGill University Health Centre, Montreal, Québec, CanadaDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, CanadaCentre for Outcomes Research and Evaluation, Research Institute of the McGill University Health Centre, Montreal, Quebec, CanadaBackground SLE is associated with increased risk of diffuse large B-cell lymphoma (DLBCL). DLBCL is routinely classified by cell of origin (COO), with germinal centre B-cell (GCB) being more common and indicating better prognosis in the general population. We studied COO subtyping in patients with SLE diagnosed with DLBCL and their survival.Patients and methods We evaluated 20 cases of SLE with DLBCL. Immunohistochemistry analysis was performed (BCL2, MYC, BCL6, CD10, CD20, FOXP1, GCET1, MUM1) in tissue microarrays. We examined associations between molecular and clinical features, including overall survival.Results Of the 20 DLBCL SLE cases, 12/20 cases (60%) were classified as non-GCB using Hans or Choi algorithms. MYC and BCL2 protein expression was positive in 6/20 (30%) and 8/20 (40%) SLE cases, respectively, with 2/20 (10%) co-expressing both markers. Seven (7/20) had only extranodal involvement at DLBCL diagnosis. As expected, non-GCB cases had worse survival. Cases presenting exclusively with extranodal disease were associated with shorter SLE duration and better survival despite higher BCL2 protein expression.Conclusions We present novel data characterising DLBCL in SLE. Sixty per cent of the DLBCL in patients with SLE were non-GCB. The nodal and extranodal distribution in SLE was similar to what is known in the general population, but extranodal disease occurred more often with short SLE duration and was associated with longer overall survival. More research on cancer in SLE is the key to further understanding the complex interplay between cancer and the immune system.https://lupus.bmj.com/content/6/1/e000324.full
spellingShingle Rosalind Ramsey-Goldman
Diane L Kamen
Eva Baecklund
Basile Tessier-Cloutier
David DW Twa
Randy Gascoyne
Nathalie A Johnson
Carin Backlin
Ann E Clarke
Jennifer LF Lee
Pedro Farinha
Sasha Bernatsky
Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival
Lupus Science and Medicine
title Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival
title_full Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival
title_fullStr Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival
title_full_unstemmed Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival
title_short Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival
title_sort cell of origin in diffuse large b cell lymphoma in systemic lupus erythematosus molecular and clinical factors associated with survival
url https://lupus.bmj.com/content/6/1/e000324.full
work_keys_str_mv AT rosalindramseygoldman celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT dianelkamen celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT evabaecklund celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT basiletessiercloutier celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT daviddwtwa celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT randygascoyne celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT nathalieajohnson celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT carinbacklin celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT anneclarke celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT jenniferlflee celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT pedrofarinha celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival
AT sashabernatsky celloforiginindiffuselargebcelllymphomainsystemiclupuserythematosusmolecularandclinicalfactorsassociatedwithsurvival