Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats
Osteoarthritis is often exacerbated by obesity and metabolic syndrome, which are frequently associated with high-fat diets (HFD). This study investigates the direct effects of HFD on articular cartilage following anterior cruciate ligament transection (ACLT) in rats, while also examining the role of...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006121 |
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| author | Hongri Ruan Tingting Zhu Tianwen Ma Yun Liu Jiasan Zheng |
| author_facet | Hongri Ruan Tingting Zhu Tianwen Ma Yun Liu Jiasan Zheng |
| author_sort | Hongri Ruan |
| collection | DOAJ |
| description | Osteoarthritis is often exacerbated by obesity and metabolic syndrome, which are frequently associated with high-fat diets (HFD). This study investigates the direct effects of HFD on articular cartilage following anterior cruciate ligament transection (ACLT) in rats, while also examining the role of free fatty acids (FFA) on chondrocyte activity. Our findings indicate that short-term HFD treatment after ACLT reduces pain sensitivity, alleviates knee swelling, and mitigates cartilage damage. Joint imaging studies, along with serum analyses of inflammatory markers and extracellular matrix (ECM) degradation, further underscore the protective role of post-surgical HFD in maintaining cartilage integrity. Notably, HFD significantly enhances AMP-activated protein kinase (AMPK) phosphorylation in cartilage. In vitro experiments reveal that low concentrations of FFAs stimulate chondrocyte proliferation and energy metabolism, whereas AMPK inhibition leads to elevated expression of inflammatory mediators and ECM-degrading enzymes in chondrocytes. Collectively, these results suggest that short-term HFD following ACLT surgery exerts protective effects on cartilage, primarily through AMPK activation. |
| format | Article |
| id | doaj-art-9c2282fa9b8d42e38cd8787e3c3ac4eb |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-9c2282fa9b8d42e38cd8787e3c3ac4eb2025-01-12T05:24:42ZengElsevierJournal of Functional Foods1756-46462025-01-01124106609Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in ratsHongri Ruan0Tingting Zhu1Tianwen Ma2Yun Liu3Jiasan Zheng4College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163000, China; Corresponding authors.Osteoarthritis is often exacerbated by obesity and metabolic syndrome, which are frequently associated with high-fat diets (HFD). This study investigates the direct effects of HFD on articular cartilage following anterior cruciate ligament transection (ACLT) in rats, while also examining the role of free fatty acids (FFA) on chondrocyte activity. Our findings indicate that short-term HFD treatment after ACLT reduces pain sensitivity, alleviates knee swelling, and mitigates cartilage damage. Joint imaging studies, along with serum analyses of inflammatory markers and extracellular matrix (ECM) degradation, further underscore the protective role of post-surgical HFD in maintaining cartilage integrity. Notably, HFD significantly enhances AMP-activated protein kinase (AMPK) phosphorylation in cartilage. In vitro experiments reveal that low concentrations of FFAs stimulate chondrocyte proliferation and energy metabolism, whereas AMPK inhibition leads to elevated expression of inflammatory mediators and ECM-degrading enzymes in chondrocytes. Collectively, these results suggest that short-term HFD following ACLT surgery exerts protective effects on cartilage, primarily through AMPK activation.http://www.sciencedirect.com/science/article/pii/S1756464624006121High-fat dietPost-traumatic osteoarthritisCartilageExtracellular matrixAMPK |
| spellingShingle | Hongri Ruan Tingting Zhu Tianwen Ma Yun Liu Jiasan Zheng Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats Journal of Functional Foods High-fat diet Post-traumatic osteoarthritis Cartilage Extracellular matrix AMPK |
| title | Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats |
| title_full | Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats |
| title_fullStr | Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats |
| title_full_unstemmed | Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats |
| title_short | Short-term high-fat diet post-ACLT surgery activates chondrocyte AMPK pathway and slows articular cartilage degeneration in rats |
| title_sort | short term high fat diet post aclt surgery activates chondrocyte ampk pathway and slows articular cartilage degeneration in rats |
| topic | High-fat diet Post-traumatic osteoarthritis Cartilage Extracellular matrix AMPK |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006121 |
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