Detection of gene mutations in genome “hot” spots: “hairpin” amplicons in DNA melting analysis
Polymerase chain reaction (PCR) followed by DNA melting analysis with TaqMan probes effectively reveals mutations in the human genome “hot” spots. The necessity to carry out PCR in the asymmetric variant causes, however, a number of restrictions of this method: 1) an inability of quantitative estima...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
ABV-press
2017-04-01
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| Series: | Успехи молекулярной онкологии |
| Subjects: | |
| Online Access: | https://umo.abvpress.ru/jour/article/view/88 |
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