Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder
This study investigates the neuroprotective effects of dexpanthenol (DEX) and butafosfan-vitmain B12 combination (BUT) in a circadian rhythm disorder (CRD)- induced brain damage model in mice. Control, CRD, DEX, BUT, and BUT + DEX groups were subjected to a 19-day experimental period during which CR...
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Kafkas University, Faculty of Veterinary Medicine
2025-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3207 |
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| author | Simge GARLI Özlem ÖZMEN Şerife TAŞAN |
| author_facet | Simge GARLI Özlem ÖZMEN Şerife TAŞAN |
| author_sort | Simge GARLI |
| collection | DOAJ |
| description | This study investigates the neuroprotective effects of dexpanthenol (DEX) and
butafosfan-vitmain B12 combination (BUT) in a circadian rhythm disorder (CRD)-
induced brain damage model in mice. Control, CRD, DEX, BUT, and BUT + DEX
groups were subjected to a 19-day experimental period during which CRD was induced
by repeated phase shifts in the light/dark cycle. The CRD group experienced circadian
rhythm disorder, while the DEX and BUT groups received intraperitoneal DEX (1000
mg/kg/day) and subcutaneous BUT (200 mg/kg/day) treatments, respectively, for the
same duration. Serum cortisol and creatine kinase (CK) levels were measured using
ELISA to assess stress and tissue damage. Brain tissues were evaluated histopathologically
using hematoxylin and eosin staining, and immunohistochemically for brain-derived
neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) expression using
specific monoclonal antibodies. CRD significantly increased serum cortisol and CK
levels compared to the control group (P<0.001). Both DEX and BUT treatments reduced
these elevations, with the combination therapy showing the most pronounced effect
(P<0.001). Histopathological examination revealed reduced neuronal degeneration,
hyperemia, and hemorrhage in the treatment groups compared to the CRD group.
Immunohistochemical analysis showed significantly increased BDNF and GFAP
expression in the BUT + DEX group (P<0.001). These findings suggest that DEX and
BUT, particularly in combination, exert neuroprotective effects against CRD-induced
brain injury by modulating oxidative stress, inflammation, and neurotrophic signaling
pathways. |
| format | Article |
| id | doaj-art-9bf3883d8ab846c38e314fc8f5e60e86 |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-9bf3883d8ab846c38e314fc8f5e60e862025-08-20T04:03:01ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512025-07-0131455756410.9775/kvfd.2025.343333207Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorderSimge GARLI0Özlem ÖZMEN1Şerife TAŞAN2Burdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Laboratory Animals Production and Experimental Research Center, TR-15030 Burdur - TÜRKİYEBurdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, TR-15030 Burdur - TÜRKİYEBurdur Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, TR-15030 Burdur - TÜRKİYEThis study investigates the neuroprotective effects of dexpanthenol (DEX) and butafosfan-vitmain B12 combination (BUT) in a circadian rhythm disorder (CRD)- induced brain damage model in mice. Control, CRD, DEX, BUT, and BUT + DEX groups were subjected to a 19-day experimental period during which CRD was induced by repeated phase shifts in the light/dark cycle. The CRD group experienced circadian rhythm disorder, while the DEX and BUT groups received intraperitoneal DEX (1000 mg/kg/day) and subcutaneous BUT (200 mg/kg/day) treatments, respectively, for the same duration. Serum cortisol and creatine kinase (CK) levels were measured using ELISA to assess stress and tissue damage. Brain tissues were evaluated histopathologically using hematoxylin and eosin staining, and immunohistochemically for brain-derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GFAP) expression using specific monoclonal antibodies. CRD significantly increased serum cortisol and CK levels compared to the control group (P<0.001). Both DEX and BUT treatments reduced these elevations, with the combination therapy showing the most pronounced effect (P<0.001). Histopathological examination revealed reduced neuronal degeneration, hyperemia, and hemorrhage in the treatment groups compared to the CRD group. Immunohistochemical analysis showed significantly increased BDNF and GFAP expression in the BUT + DEX group (P<0.001). These findings suggest that DEX and BUT, particularly in combination, exert neuroprotective effects against CRD-induced brain injury by modulating oxidative stress, inflammation, and neurotrophic signaling pathways.https://vetdergikafkas.org/pdf.php?id=3207butafosfancircadian rhythmdexpanthenolneuroprotectivevitamin b12 |
| spellingShingle | Simge GARLI Özlem ÖZMEN Şerife TAŞAN Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder Kafkas Universitesi Veteriner Fakültesi Dergisi butafosfan circadian rhythm dexpanthenol neuroprotective vitamin b12 |
| title | Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder |
| title_full | Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder |
| title_fullStr | Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder |
| title_full_unstemmed | Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder |
| title_short | Neuroprotective role of dexpanthenol and butafosfan-vitamin b12 against brain damage induced by circadian rhythm disorder |
| title_sort | neuroprotective role of dexpanthenol and butafosfan vitamin b12 against brain damage induced by circadian rhythm disorder |
| topic | butafosfan circadian rhythm dexpanthenol neuroprotective vitamin b12 |
| url | https://vetdergikafkas.org/pdf.php?id=3207 |
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