Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species
This study investigates <i>Xanthomonas campestris</i> pv. <i>campestris</i> (Xcc) incidence rates in cabbage, kale, and kohlrabi, focusing on the roots and stems over a 15-day period after sowing. Seeds were inoculated with Xcc, and infection levels were monitored using confo...
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MDPI AG
2024-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/12/1307 |
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| author | Vojtěch Ferby Lucia Nedorost Ragasová Jakub Pečenka Agnieszka Sękara Robert Pokluda |
| author_facet | Vojtěch Ferby Lucia Nedorost Ragasová Jakub Pečenka Agnieszka Sękara Robert Pokluda |
| author_sort | Vojtěch Ferby |
| collection | DOAJ |
| description | This study investigates <i>Xanthomonas campestris</i> pv. <i>campestris</i> (Xcc) incidence rates in cabbage, kale, and kohlrabi, focusing on the roots and stems over a 15-day period after sowing. Seeds were inoculated with Xcc, and infection levels were monitored using confocal microscopy combined with fluorescence in situ hybridization. Significant differences in incidence rates were observed across the 15 days using a scale from 0 to 5 (0–100% incidence). Kale exhibited the highest mean incidence rates in roots (3.64), while cabbage and kohlrabi showed greater variability (3.4–3.44). Stem infections were more severe, with kale showing the highest mean levels (4.16), followed by kohlrabi (4.0), and cabbage (3.68). In cabbage roots, incidence rates increased until day 11, after which a significant decline was noted, whereas stem Xcc incidence rates remained stable. Kale roots peaked on day 9, with significant fluctuations on days 13 and 15. Kohlrabi displayed moderate, stable root incidence rates over the 15-day period. In stems, kohlrabi showed a significant increase on day 11, followed by a drop on day 15. Regression analysis revealed a significant positive linear correlation between incidence rate and days of observation in kohlrabi roots. However, no significant trends were observed in stem infections across all species, where incidence rates were high and stable from day 7 onwards. Newly acquired data indicate that the incidence rate of Xcc development depends on the type of vegetable. However, the bacterial occurrence in the root parts does not always correspond with the intensity of Xcc colonization in the stem section of the plants. Even at lower levels of Xcc occurrence in the roots, damage to the vascular bundles can be fatal. These findings offer valuable insights into Xcc infection dynamics, contributing to improved disease management strategies for <i>Brassica</i> crops. |
| format | Article |
| id | doaj-art-9bbea4c761db4ad89373a171a7c0a7a9 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-9bbea4c761db4ad89373a171a7c0a7a92024-12-27T14:29:15ZengMDPI AGHorticulturae2311-75242024-12-011012130710.3390/horticulturae10121307Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> SpeciesVojtěch Ferby0Lucia Nedorost Ragasová1Jakub Pečenka2Agnieszka Sękara3Robert Pokluda4Department of Vegetable Science and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicDepartment of Vegetable Science and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicMendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicDepartment of Horticulture, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, 31425 Krakow, PolandDepartment of Vegetable Science and Floriculture, Faculty of Horticulture, Mendel University in Brno, Valtická 337, 691 44 Lednice, Czech RepublicThis study investigates <i>Xanthomonas campestris</i> pv. <i>campestris</i> (Xcc) incidence rates in cabbage, kale, and kohlrabi, focusing on the roots and stems over a 15-day period after sowing. Seeds were inoculated with Xcc, and infection levels were monitored using confocal microscopy combined with fluorescence in situ hybridization. Significant differences in incidence rates were observed across the 15 days using a scale from 0 to 5 (0–100% incidence). Kale exhibited the highest mean incidence rates in roots (3.64), while cabbage and kohlrabi showed greater variability (3.4–3.44). Stem infections were more severe, with kale showing the highest mean levels (4.16), followed by kohlrabi (4.0), and cabbage (3.68). In cabbage roots, incidence rates increased until day 11, after which a significant decline was noted, whereas stem Xcc incidence rates remained stable. Kale roots peaked on day 9, with significant fluctuations on days 13 and 15. Kohlrabi displayed moderate, stable root incidence rates over the 15-day period. In stems, kohlrabi showed a significant increase on day 11, followed by a drop on day 15. Regression analysis revealed a significant positive linear correlation between incidence rate and days of observation in kohlrabi roots. However, no significant trends were observed in stem infections across all species, where incidence rates were high and stable from day 7 onwards. Newly acquired data indicate that the incidence rate of Xcc development depends on the type of vegetable. However, the bacterial occurrence in the root parts does not always correspond with the intensity of Xcc colonization in the stem section of the plants. Even at lower levels of Xcc occurrence in the roots, damage to the vascular bundles can be fatal. These findings offer valuable insights into Xcc infection dynamics, contributing to improved disease management strategies for <i>Brassica</i> crops.https://www.mdpi.com/2311-7524/10/12/1307black rot diseasecabbagekalekohlrabiconfocal microscopy |
| spellingShingle | Vojtěch Ferby Lucia Nedorost Ragasová Jakub Pečenka Agnieszka Sękara Robert Pokluda Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species Horticulturae black rot disease cabbage kale kohlrabi confocal microscopy |
| title | Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species |
| title_full | Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species |
| title_fullStr | Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species |
| title_full_unstemmed | Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species |
| title_short | Early Stages of Bacterial Colonization Development After <em>Xanthomonas campestris</em> Seed Inoculation in Selected <em>Brassica</em> Species |
| title_sort | early stages of bacterial colonization development after em xanthomonas campestris em seed inoculation in selected em brassica em species |
| topic | black rot disease cabbage kale kohlrabi confocal microscopy |
| url | https://www.mdpi.com/2311-7524/10/12/1307 |
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