Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18)
Bacterial communication, Quorum Sensing (QS), is a target against virulence and prevention of antibiotic-resistant infections. 16 derivatives of Piperlongumine (PL), an amide alkaloid from Piper longum L., were screened for QS inhibition. PL-18 had the best QSI activity. PL-18 inhibited the lasR-las...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207524000406 |
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| author | Yael Schlichter Kadosh Subramani Muthuraman Khairun Nisaa Anat Ben-Zvi Danit Lisa Karsagi Byron Marilou Shagan Alexander Brandis Tevie Mehlman Jacob Gopas Rajendran Saravana Kumar Ariel Kushmaro |
| author_facet | Yael Schlichter Kadosh Subramani Muthuraman Khairun Nisaa Anat Ben-Zvi Danit Lisa Karsagi Byron Marilou Shagan Alexander Brandis Tevie Mehlman Jacob Gopas Rajendran Saravana Kumar Ariel Kushmaro |
| author_sort | Yael Schlichter Kadosh |
| collection | DOAJ |
| description | Bacterial communication, Quorum Sensing (QS), is a target against virulence and prevention of antibiotic-resistant infections. 16 derivatives of Piperlongumine (PL), an amide alkaloid from Piper longum L., were screened for QS inhibition. PL-18 had the best QSI activity. PL-18 inhibited the lasR-lasI, rhlR-rhlI, and pqs QS systems of Pseudomonas aeruginosa. PL-18 inhibited pyocyanin and rhamnolipids that are QS-controlled virulence elements. Iron is an essential element for pathogenicity, biofilm formation and resilience in harsh environments, its uptake was inhibited by PL-18. Pl-18 significantly reduced the biofilm biovolume including in established biofilms. PL-18-coated silicon tubes significantly inhibited biofilm formation. The transcriptome study of treated P. aeruginosa showed that PL-18 indeed reduced the expression of QS and iron homeostasis related genes, and up regulated sulfur metabolism related genes. Altogether, PL-18 inhibits QS, virulence, iron uptake, and biofilm formation. Thus, PL-18 should be further developed against bacterial infection, antibiotic resistance, and biofilm formation. |
| format | Article |
| id | doaj-art-9bb55de43cda4bef8bdd979c928ffac3 |
| institution | Kabale University |
| issn | 2590-2075 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-9bb55de43cda4bef8bdd979c928ffac32024-12-12T05:22:44ZengElsevierBiofilm2590-20752024-12-018100215Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18)Yael Schlichter Kadosh0Subramani Muthuraman1Khairun Nisaa2Anat Ben-Zvi3Danit Lisa Karsagi Byron4Marilou Shagan5Alexander Brandis6Tevie Mehlman7Jacob Gopas8Rajendran Saravana Kumar9Ariel Kushmaro10Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, IsraelChemistry Division, Vellore Institute of Technology, Chennai, IndiaDepartment of Life Science, Ben-Gurion University of the Negev, Beer Sheva, IsraelDepartment of Life Science, Ben-Gurion University of the Negev, Beer Sheva, IsraelDepartment of Civil and Environmental Engineering, Ben-Gurion University of the Negev, Beer Sheva, IsraelAvram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, IsraelDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, IsraelDepartment of Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, IsraelDepartment of Microbiology, Immunology and Genetics Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, IsraelChemistry Division, Vellore Institute of Technology, Chennai, India; Corresponding author.Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel; The Ilse Katz Center for Nanoscale Science and Technology, Ben Gurion University of the Negev, Beer Sheva, Israel; School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva, Israel; Corresponding author. Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben Gurion University of the Negev, Beer Sheva, Israel.Bacterial communication, Quorum Sensing (QS), is a target against virulence and prevention of antibiotic-resistant infections. 16 derivatives of Piperlongumine (PL), an amide alkaloid from Piper longum L., were screened for QS inhibition. PL-18 had the best QSI activity. PL-18 inhibited the lasR-lasI, rhlR-rhlI, and pqs QS systems of Pseudomonas aeruginosa. PL-18 inhibited pyocyanin and rhamnolipids that are QS-controlled virulence elements. Iron is an essential element for pathogenicity, biofilm formation and resilience in harsh environments, its uptake was inhibited by PL-18. Pl-18 significantly reduced the biofilm biovolume including in established biofilms. PL-18-coated silicon tubes significantly inhibited biofilm formation. The transcriptome study of treated P. aeruginosa showed that PL-18 indeed reduced the expression of QS and iron homeostasis related genes, and up regulated sulfur metabolism related genes. Altogether, PL-18 inhibits QS, virulence, iron uptake, and biofilm formation. Thus, PL-18 should be further developed against bacterial infection, antibiotic resistance, and biofilm formation.http://www.sciencedirect.com/science/article/pii/S2590207524000406PiperlonguminePiper longumAmide alkaloidPseudomonas aeruginosaQuorum sensingBacterial biofilm |
| spellingShingle | Yael Schlichter Kadosh Subramani Muthuraman Khairun Nisaa Anat Ben-Zvi Danit Lisa Karsagi Byron Marilou Shagan Alexander Brandis Tevie Mehlman Jacob Gopas Rajendran Saravana Kumar Ariel Kushmaro Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18) Biofilm Piperlongumine Piper longum Amide alkaloid Pseudomonas aeruginosa Quorum sensing Bacterial biofilm |
| title | Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18) |
| title_full | Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18) |
| title_fullStr | Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18) |
| title_full_unstemmed | Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18) |
| title_short | Pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di-hydroxy derivative of piperlongumine (PL-18) |
| title_sort | pseudomonas aeruginosa quorum sensing and biofilm attenuation by a di hydroxy derivative of piperlongumine pl 18 |
| topic | Piperlongumine Piper longum Amide alkaloid Pseudomonas aeruginosa Quorum sensing Bacterial biofilm |
| url | http://www.sciencedirect.com/science/article/pii/S2590207524000406 |
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