Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone
Background: Nasopharyngeal colonization with Staphylococcus aureus is a risk factor for subsequent infection. Isolates from colonization can therefore provide important information on virulence factors and antimicrobial resistance when data from clinical isolates are lacking. The aim of this study w...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | International Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S143842212400047X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841558855510654976 |
|---|---|
| author | Lisa Maria Kleine Emmanuel Marx Kanu Tobias Grebe Desmond Mohamed Sesay Henning Loismann Maxwell Sesay Tom Theiler Viktoria Rudolf Alexander Mellmann Laura C. Kalkman Martin P. Grobusch Frieder Schaumburg |
| author_facet | Lisa Maria Kleine Emmanuel Marx Kanu Tobias Grebe Desmond Mohamed Sesay Henning Loismann Maxwell Sesay Tom Theiler Viktoria Rudolf Alexander Mellmann Laura C. Kalkman Martin P. Grobusch Frieder Schaumburg |
| author_sort | Lisa Maria Kleine |
| collection | DOAJ |
| description | Background: Nasopharyngeal colonization with Staphylococcus aureus is a risk factor for subsequent infection. Isolates from colonization can therefore provide important information on virulence factors and antimicrobial resistance when data from clinical isolates are lacking. The aim of this study was to assess colonization rates, resistance patterns and selected virulence factors of S. aureus from rural Sierra Leone. Methods: Residents of randomly selected houses in Masanga, Sierra Leone were included in a cross-sectional study (8–11/2023). Participants were tested for nasopharyngeal S. aureus colonization using selective culture media. Risk factors for colonization were documented in a standardized questionnaire. Isolates were genotyped and tested for antimicrobial susceptibility and selected virulence factors (e.g. Panton-Valentine leukocidin, capsular types). Results: Of 300 participants (62.7 % females, median age: 16 years), 168 (56 %) were colonized with S. aureus-related complex; six participants carried two different S. aureus genotypes, resulting in a total number of 174 isolates. Resistance to penicillin was predominant (97.1 %, 169/174), followed by tetracycline (66.1 %, 115/174), co-trimoxazole (56.9 %, 99/174) and oxacillin (24.1 %, 42/174, all mecA-positive, mostly associated with ST8/PVL-negative). PVL gene was detected in 21.3 % of isolates (37/174) mainly associated with ST15 and ST152. Except for past use of antimicrobials (p = 0.019), no specific risk factors such as comorbidities including hemoglobin variants were associated with S. aureus nasopharyngeal colonization. Conclusion: The prevalence of methicillin-resistant and PVL-positive methicillin-susceptible S. aureus (MRSA/MSSA) is high in a rural community of asymptomatic carriers in Sierra Leone. Measures to contain the spread of MRSA, also in the community, are needed. |
| format | Article |
| id | doaj-art-9bab75a697654f2f945d16bb7d8e2ab9 |
| institution | Kabale University |
| issn | 1438-4221 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Medical Microbiology |
| spelling | doaj-art-9bab75a697654f2f945d16bb7d8e2ab92025-01-06T04:08:33ZengElsevierInternational Journal of Medical Microbiology1438-42212025-03-01318151643Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra LeoneLisa Maria Kleine0Emmanuel Marx Kanu1Tobias Grebe2Desmond Mohamed Sesay3Henning Loismann4Maxwell Sesay5Tom Theiler6Viktoria Rudolf7Alexander Mellmann8Laura C. Kalkman9Martin P. Grobusch10Frieder Schaumburg11Institute of Medical Microbiology, University Hospital Münster, Münster, GermanyMasanga Medical Research Unit, Masanga Hospital, Masanga, Sierra Leone; Centre of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, Location AMC, Amsterdam Infection and Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, the NetherlandsInstitute of Medical Microbiology, University Hospital Münster, Münster, Germany; Corresponding author.Masanga Medical Research Unit, Masanga Hospital, Masanga, Sierra LeoneInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyMasanga Medical Research Unit, Masanga Hospital, Masanga, Sierra LeoneInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyInstitute of Medical Microbiology, University Hospital Münster, Münster, GermanyInstitute of Hygiene, University Hospital Münster, Münster, GermanyCentre of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, Location AMC, Amsterdam Infection and Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, the NetherlandsMasanga Medical Research Unit, Masanga Hospital, Masanga, Sierra Leone; Centre of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, Location AMC, Amsterdam Infection and Immunity, Amsterdam Public Health, University of Amsterdam, Amsterdam, the Netherlands; Institute of Tropical Medicine & Deutsches Zentrum für Infektionsforschung, University of Tübingen, Tübingen, Germany; Centre de Recherches Médicales, Lambaréné, Gabon; Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South AfricaInstitute of Medical Microbiology, University Hospital Münster, Münster, Germany; Masanga Medical Research Unit, Masanga Hospital, Masanga, Sierra LeoneBackground: Nasopharyngeal colonization with Staphylococcus aureus is a risk factor for subsequent infection. Isolates from colonization can therefore provide important information on virulence factors and antimicrobial resistance when data from clinical isolates are lacking. The aim of this study was to assess colonization rates, resistance patterns and selected virulence factors of S. aureus from rural Sierra Leone. Methods: Residents of randomly selected houses in Masanga, Sierra Leone were included in a cross-sectional study (8–11/2023). Participants were tested for nasopharyngeal S. aureus colonization using selective culture media. Risk factors for colonization were documented in a standardized questionnaire. Isolates were genotyped and tested for antimicrobial susceptibility and selected virulence factors (e.g. Panton-Valentine leukocidin, capsular types). Results: Of 300 participants (62.7 % females, median age: 16 years), 168 (56 %) were colonized with S. aureus-related complex; six participants carried two different S. aureus genotypes, resulting in a total number of 174 isolates. Resistance to penicillin was predominant (97.1 %, 169/174), followed by tetracycline (66.1 %, 115/174), co-trimoxazole (56.9 %, 99/174) and oxacillin (24.1 %, 42/174, all mecA-positive, mostly associated with ST8/PVL-negative). PVL gene was detected in 21.3 % of isolates (37/174) mainly associated with ST15 and ST152. Except for past use of antimicrobials (p = 0.019), no specific risk factors such as comorbidities including hemoglobin variants were associated with S. aureus nasopharyngeal colonization. Conclusion: The prevalence of methicillin-resistant and PVL-positive methicillin-susceptible S. aureus (MRSA/MSSA) is high in a rural community of asymptomatic carriers in Sierra Leone. Measures to contain the spread of MRSA, also in the community, are needed.http://www.sciencedirect.com/science/article/pii/S143842212400047XStaphylococcus aureusSub-Sahara AfricaColonizationPanton-Valentine leukocidinAntimicrobial resistanceMethicillin-resistance |
| spellingShingle | Lisa Maria Kleine Emmanuel Marx Kanu Tobias Grebe Desmond Mohamed Sesay Henning Loismann Maxwell Sesay Tom Theiler Viktoria Rudolf Alexander Mellmann Laura C. Kalkman Martin P. Grobusch Frieder Schaumburg Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone International Journal of Medical Microbiology Staphylococcus aureus Sub-Sahara Africa Colonization Panton-Valentine leukocidin Antimicrobial resistance Methicillin-resistance |
| title | Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone |
| title_full | Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone |
| title_fullStr | Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone |
| title_full_unstemmed | Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone |
| title_short | Nasopharyngeal carriage of Staphylococcus aureus in a rural population, Sierra Leone |
| title_sort | nasopharyngeal carriage of staphylococcus aureus in a rural population sierra leone |
| topic | Staphylococcus aureus Sub-Sahara Africa Colonization Panton-Valentine leukocidin Antimicrobial resistance Methicillin-resistance |
| url | http://www.sciencedirect.com/science/article/pii/S143842212400047X |
| work_keys_str_mv | AT lisamariakleine nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT emmanuelmarxkanu nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT tobiasgrebe nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT desmondmohamedsesay nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT henningloismann nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT maxwellsesay nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT tomtheiler nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT viktoriarudolf nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT alexandermellmann nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT laurackalkman nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT martinpgrobusch nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone AT friederschaumburg nasopharyngealcarriageofstaphylococcusaureusinaruralpopulationsierraleone |