EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
Abstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the fol...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/70 |
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| author | S. V. Khaidukov A. V. Zurochka |
| author_facet | S. V. Khaidukov A. V. Zurochka |
| author_sort | S. V. Khaidukov |
| collection | DOAJ |
| description | Abstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the following approcahes: detection of antigen-specific T-cells by using of tetramer technology, flow cytometric determination of cytokines in biological liquids, determination of in vitro sensitivity of basophilic granulocytes to allergen effects, detection of Th1 and Т-reg cells by their cell surface markers. When using multiparametric analysis, a multi-step gating and other new technologies, the flow cytometry technique allows of location and tracing the majority of processes occurring in development of immune response. When studying these dynamic events, we get an opportunity to react adequately to apropriate changes, and to develop new approaches to correct altered cellular activities, that they should determinate. |
| format | Article |
| id | doaj-art-9ba72e9cba3c40428bfac6d8d8c3023e |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-9ba72e9cba3c40428bfac6d8d8c3023e2025-08-20T04:00:09ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0110151210.15789/1563-0625-2008-1-5-1268EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICES. V. Khaidukov0A. V. Zurochka1Институт биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, МоскваГОУ ВПО Челябинская государственная медицинская академия Росздрава, г. ЧелябинскAbstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the following approcahes: detection of antigen-specific T-cells by using of tetramer technology, flow cytometric determination of cytokines in biological liquids, determination of in vitro sensitivity of basophilic granulocytes to allergen effects, detection of Th1 and Т-reg cells by their cell surface markers. When using multiparametric analysis, a multi-step gating and other new technologies, the flow cytometry technique allows of location and tracing the majority of processes occurring in development of immune response. When studying these dynamic events, we get an opportunity to react adequately to apropriate changes, and to develop new approaches to correct altered cellular activities, that they should determinate.https://www.mimmun.ru/mimmun/article/view/70flow cytometrytetramer technologybasophilscytokinesmultiparametric analysis |
| spellingShingle | S. V. Khaidukov A. V. Zurochka EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE Медицинская иммунология flow cytometry tetramer technology basophils cytokines multiparametric analysis |
| title | EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE |
| title_full | EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE |
| title_fullStr | EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE |
| title_full_unstemmed | EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE |
| title_short | EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE |
| title_sort | expansion of opportunities of the flow cytometry method for clinical and immunologycal practice |
| topic | flow cytometry tetramer technology basophils cytokines multiparametric analysis |
| url | https://www.mimmun.ru/mimmun/article/view/70 |
| work_keys_str_mv | AT svkhaidukov expansionofopportunitiesoftheflowcytometrymethodforclinicalandimmunologycalpractice AT avzurochka expansionofopportunitiesoftheflowcytometrymethodforclinicalandimmunologycalpractice |