Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats
Background & Aims: Laportea aestuans is a medicinal plant used in ethnomedicine as an abortifacient, anthelminthic, anti-fibroid, antipyretic, and anti-microbial agent. Uterine leiomyoma, a menace of reproductive age in women, is characterized by the proliferation of smooth muscle cells (hyperpl...
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Urmia University of Medical Sciences
2024-11-01
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| Series: | Journal of Research in Applied and Basic Medical Sciences |
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| Online Access: | http://ijrabms.umsu.ac.ir/article-1-336-en.pdf |
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| author | Qudus Ojomo Esther Agbaje Joseph Olamijulo |
| author_facet | Qudus Ojomo Esther Agbaje Joseph Olamijulo |
| author_sort | Qudus Ojomo |
| collection | DOAJ |
| description | Background & Aims: Laportea aestuans is a medicinal plant used in ethnomedicine as an abortifacient, anthelminthic, anti-fibroid, antipyretic, and anti-microbial agent. Uterine leiomyoma, a menace of reproductive age in women, is characterized by the proliferation of smooth muscle cells (hyperplasia) in the uterus. This study aims to investigate the preventive and curative anti-leiomyoma activity of Laportea aestuans using monosodium glutamate-induced uterine hyperplasia models in female Sprague-Dawley rats.
Materials & Methods: The methanol whole plant extract of Laportea aestuans (MELA) was prepared by extracting the pulverized, air-dried whole plant with 2.5 L of methanol in a Soxhlet apparatus, followed by filtration and oven-drying. The acute toxicity of MELA was then assessed using Lorke’s method. Subsequently, three doses of MELA were selected for this study.
Uterine hyperplasia was induced with 200 mg/kg p.o. monosodium glutamate (MSG) for 30 days. MELA was either co-administered with the inducing agent (preventive study) or administered post-induction for another 30 days (curative study). The anti-leiomyoma activity of MELA was then assessed through haematological, biochemical, and histopathological findings.
Results: Increased serum oestrogen, progesterone, and total cholesterol levels were observed in the untreated fibroid animal groups, but these were significantly attenuated (p < 0.05) in animals treated with different doses of MELA. This correlated with the histopathological findings, as MELA reversed uterine hyperplasia with its therapeutic potential noted from 500 mg/kg.
Conclusion: These findings suggest that MELA contains bioactive agents that can reverse MSG-induced uterine hyperplasia. It may therefore be useful in reducing the proliferation of fibroblast cells and managing other symptoms associated with uterine leiomyoma upon successful clinical trials. |
| format | Article |
| id | doaj-art-9b9bad4e7e874d088352bb73623a50d7 |
| institution | Kabale University |
| issn | 2717-0098 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Urmia University of Medical Sciences |
| record_format | Article |
| series | Journal of Research in Applied and Basic Medical Sciences |
| spelling | doaj-art-9b9bad4e7e874d088352bb73623a50d72024-11-30T04:46:05ZengUrmia University of Medical SciencesJournal of Research in Applied and Basic Medical Sciences2717-00982024-11-01104361379Methanol extract of Laportea aestuans reverses uterine hyperplasia in ratsQudus Ojomo0Esther Agbaje1Joseph Olamijulo2 Department of Pharmacology, Therapeutics and Toxicology, College of Medicine, University of Lagos, Idi Araba, Lagos, Nigeria African Centre of Excellence for Drug Research, Medicine Development and Regulator Science, University of Lagos, Akoka, Lagos, Nigeria Department of Obstetrics and Gynecology, College of Medicine, University of Lagos, Idi Araba, Lagos, Nigeria Background & Aims: Laportea aestuans is a medicinal plant used in ethnomedicine as an abortifacient, anthelminthic, anti-fibroid, antipyretic, and anti-microbial agent. Uterine leiomyoma, a menace of reproductive age in women, is characterized by the proliferation of smooth muscle cells (hyperplasia) in the uterus. This study aims to investigate the preventive and curative anti-leiomyoma activity of Laportea aestuans using monosodium glutamate-induced uterine hyperplasia models in female Sprague-Dawley rats. Materials & Methods: The methanol whole plant extract of Laportea aestuans (MELA) was prepared by extracting the pulverized, air-dried whole plant with 2.5 L of methanol in a Soxhlet apparatus, followed by filtration and oven-drying. The acute toxicity of MELA was then assessed using Lorke’s method. Subsequently, three doses of MELA were selected for this study. Uterine hyperplasia was induced with 200 mg/kg p.o. monosodium glutamate (MSG) for 30 days. MELA was either co-administered with the inducing agent (preventive study) or administered post-induction for another 30 days (curative study). The anti-leiomyoma activity of MELA was then assessed through haematological, biochemical, and histopathological findings. Results: Increased serum oestrogen, progesterone, and total cholesterol levels were observed in the untreated fibroid animal groups, but these were significantly attenuated (p < 0.05) in animals treated with different doses of MELA. This correlated with the histopathological findings, as MELA reversed uterine hyperplasia with its therapeutic potential noted from 500 mg/kg. Conclusion: These findings suggest that MELA contains bioactive agents that can reverse MSG-induced uterine hyperplasia. It may therefore be useful in reducing the proliferation of fibroblast cells and managing other symptoms associated with uterine leiomyoma upon successful clinical trials.http://ijrabms.umsu.ac.ir/article-1-336-en.pdffibroidlaportea aestuansmonosodium glutamatemsg-induced uterine hyperplasiaoestrogenprogesteroneuterine hyperplasiauterine leiomyomasex hormones |
| spellingShingle | Qudus Ojomo Esther Agbaje Joseph Olamijulo Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats Journal of Research in Applied and Basic Medical Sciences fibroid laportea aestuans monosodium glutamate msg-induced uterine hyperplasia oestrogen progesterone uterine hyperplasia uterine leiomyoma sex hormones |
| title | Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats |
| title_full | Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats |
| title_fullStr | Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats |
| title_full_unstemmed | Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats |
| title_short | Methanol extract of Laportea aestuans reverses uterine hyperplasia in rats |
| title_sort | methanol extract of laportea aestuans reverses uterine hyperplasia in rats |
| topic | fibroid laportea aestuans monosodium glutamate msg-induced uterine hyperplasia oestrogen progesterone uterine hyperplasia uterine leiomyoma sex hormones |
| url | http://ijrabms.umsu.ac.ir/article-1-336-en.pdf |
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