Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants
Minghuai 1 (MH1) is a yam (Dioscorea alata) cultivar characterized by its strong resistance to Cd stress. This study aims to investigate the enzymatic antioxidant system of MH1 under Cd stress. Under Cd treatment, MH1 exhibited an elevation in superoxide dismutase (SOD) activity, while the activitie...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/17429145.2023.2300513 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846106581336326144 |
|---|---|
| author | Shumei Hua Hsin-Hung Lin Zhehong Huang Mengyao Wang Qing Li Shi-Peng Chen |
| author_facet | Shumei Hua Hsin-Hung Lin Zhehong Huang Mengyao Wang Qing Li Shi-Peng Chen |
| author_sort | Shumei Hua |
| collection | DOAJ |
| description | Minghuai 1 (MH1) is a yam (Dioscorea alata) cultivar characterized by its strong resistance to Cd stress. This study aims to investigate the enzymatic antioxidant system of MH1 under Cd stress. Under Cd treatment, MH1 exhibited an elevation in superoxide dismutase (SOD) activity, while the activities of ascorbate peroxidase, catalase, and peroxidase remained unchanged. A Cu/Zn-SOD gene, DaSOD1, was cloned and found to be up-regulated following Cd treatment. The DaSOD1 gene was further introduced into tobacco plants for functional analysis. Transgenic tobacco seedlings overexpressing DaSOD1 under Cd treatments exhibited increased chlorophyll contents and seed germination rate, while the levels of superoxide anion and malondialdehyde decreased compared to the wild-type plants. This suggests that DaSOD1 overexpression mitigated the negative effects of Cd stress by reducing oxidative damage in plants. Characterization of the SOD activity and its corresponding DaSOD1 gene is expected to improve our understanding of Cd-resistance mechanism in yam plants. |
| format | Article |
| id | doaj-art-9b6d92b068f043bcbe0d23ee82b99b46 |
| institution | Kabale University |
| issn | 1742-9145 1742-9153 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj-art-9b6d92b068f043bcbe0d23ee82b99b462024-12-27T12:59:05ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532024-12-0119110.1080/17429145.2023.2300513Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plantsShumei Hua0Hsin-Hung Lin1Zhehong Huang2Mengyao Wang3Qing Li4Shi-Peng Chen5Institute of Dryland Crops, Sanming Academy of Agricultural Sciences, Sanming, Fujian, People’s Republic of ChinaDepartment of Agronomy, National Chung Hsing University, Taichung, TaiwanInstitute of Dryland Crops, Sanming Academy of Agricultural Sciences, Sanming, Fujian, People’s Republic of ChinaInstitute of Dryland Crops, Sanming Academy of Agricultural Sciences, Sanming, Fujian, People’s Republic of ChinaInstitute of Dryland Crops, Sanming Academy of Agricultural Sciences, Sanming, Fujian, People’s Republic of ChinaDepartment of Horticulture and Biotechnology, Chinese Culture University, Taipei, TaiwanMinghuai 1 (MH1) is a yam (Dioscorea alata) cultivar characterized by its strong resistance to Cd stress. This study aims to investigate the enzymatic antioxidant system of MH1 under Cd stress. Under Cd treatment, MH1 exhibited an elevation in superoxide dismutase (SOD) activity, while the activities of ascorbate peroxidase, catalase, and peroxidase remained unchanged. A Cu/Zn-SOD gene, DaSOD1, was cloned and found to be up-regulated following Cd treatment. The DaSOD1 gene was further introduced into tobacco plants for functional analysis. Transgenic tobacco seedlings overexpressing DaSOD1 under Cd treatments exhibited increased chlorophyll contents and seed germination rate, while the levels of superoxide anion and malondialdehyde decreased compared to the wild-type plants. This suggests that DaSOD1 overexpression mitigated the negative effects of Cd stress by reducing oxidative damage in plants. Characterization of the SOD activity and its corresponding DaSOD1 gene is expected to improve our understanding of Cd-resistance mechanism in yam plants.https://www.tandfonline.com/doi/10.1080/17429145.2023.2300513CadmiumDioscorea alataoxidative stresssuperoxide dismutasetransgenic plants |
| spellingShingle | Shumei Hua Hsin-Hung Lin Zhehong Huang Mengyao Wang Qing Li Shi-Peng Chen Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants Journal of Plant Interactions Cadmium Dioscorea alata oxidative stress superoxide dismutase transgenic plants |
| title | Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants |
| title_full | Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants |
| title_fullStr | Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants |
| title_full_unstemmed | Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants |
| title_short | Cloning and overexpression of the DaSOD1 gene from Dioscorea alata improves cadmium resistance in transgenic tobacco plants |
| title_sort | cloning and overexpression of the dasod1 gene from dioscorea alata improves cadmium resistance in transgenic tobacco plants |
| topic | Cadmium Dioscorea alata oxidative stress superoxide dismutase transgenic plants |
| url | https://www.tandfonline.com/doi/10.1080/17429145.2023.2300513 |
| work_keys_str_mv | AT shumeihua cloningandoverexpressionofthedasod1genefromdioscoreaalataimprovescadmiumresistanceintransgenictobaccoplants AT hsinhunglin cloningandoverexpressionofthedasod1genefromdioscoreaalataimprovescadmiumresistanceintransgenictobaccoplants AT zhehonghuang cloningandoverexpressionofthedasod1genefromdioscoreaalataimprovescadmiumresistanceintransgenictobaccoplants AT mengyaowang cloningandoverexpressionofthedasod1genefromdioscoreaalataimprovescadmiumresistanceintransgenictobaccoplants AT qingli cloningandoverexpressionofthedasod1genefromdioscoreaalataimprovescadmiumresistanceintransgenictobaccoplants AT shipengchen cloningandoverexpressionofthedasod1genefromdioscoreaalataimprovescadmiumresistanceintransgenictobaccoplants |