Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells
Lutein is a naturally occurring carotenoid with antioxidative, antitumorigenic, antiangiogenic, photoprotective, hepatoprotective, and neuroprotective properties. Although the anti-inflammatory effects of lutein have previously been described, the mechanism of its anti-inflammatory action has not be...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/787042 |
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| author | Jueun Oh Ji Hye Kim Jae Gwang Park Young-Su Yi Kye Won Park Ho Sik Rho Min-Seuk Lee Jae Won Yoo Seung-Hyun Kang Yong Deog Hong Song Seok Shin Jae Youl Cho |
| author_facet | Jueun Oh Ji Hye Kim Jae Gwang Park Young-Su Yi Kye Won Park Ho Sik Rho Min-Seuk Lee Jae Won Yoo Seung-Hyun Kang Yong Deog Hong Song Seok Shin Jae Youl Cho |
| author_sort | Jueun Oh |
| collection | DOAJ |
| description | Lutein is a naturally occurring carotenoid with antioxidative, antitumorigenic, antiangiogenic, photoprotective, hepatoprotective, and neuroprotective properties. Although the anti-inflammatory effects of lutein have previously been described, the mechanism of its anti-inflammatory action has not been fully elucidated. Therefore, in the present study, we aimed to investigate the regulatory activity of lutein in the inflammatory responses of skin-derived keratinocytes or macrophages and to elucidate the mechanism of its inhibitory action. Lutein significantly reduced several skin inflammatory responses, including increased expression of interleukin-(IL-) 6 from LPS-treated macrophages, upregulation of cyclooxygenase-(COX-) 2 from interferon-γ/tumor necrosis-factor-(TNF-) α-treated HaCaT cells, and the enhancement of matrix-metallopeptidase-(MMP-) 9 level in UV-irradiated keratinocytes. By evaluating the intracellular signaling pathway and the nuclear transcription factor levels, we determined that lutein inhibited the activation of redox-sensitive AP-1 pathway by suppressing the activation of p38 and c-Jun-N-terminal kinase (JNK). Evaluation of the radical and ROS scavenging activities further revealed that lutein was able to act as a strong anti-oxidant. Taken together, our findings strongly suggest that lutein-mediated AP-1 suppression and anti-inflammatory activity are the result of its strong antioxidative and p38/JNK inhibitory activities. These findings can be applied for the preparation of anti-inflammatory and cosmetic remedies for inflammatory diseases of the skin. |
| format | Article |
| id | doaj-art-9b6c0f5036a24935881567044b7bf464 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9b6c0f5036a24935881567044b7bf4642025-02-03T05:47:21ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/787042787042Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic CellsJueun Oh0Ji Hye Kim1Jae Gwang Park2Young-Su Yi3Kye Won Park4Ho Sik Rho5Min-Seuk Lee6Jae Won Yoo7Seung-Hyun Kang8Yong Deog Hong9Song Seok Shin10Jae Youl Cho11Department of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of KoreaDepartment of Food Science and Biotechnology, Sungkyunkwan University, Suwon 440-746, Republic of KoreaMedical Beauty Research Institute, AmorePacific R&D Center, Yongin 446-729, Republic of KoreaSulloccha Research Center, Jangwon. Co., Ltd., Jeju 699-924, Republic of KoreaMedical Beauty Research Institute, AmorePacific R&D Center, Yongin 446-729, Republic of KoreaCosmetics & Personal Care Research Division, Amorepacific R&D Center, Yongin 446-729, Republic of KoreaMedical Beauty Research Institute, AmorePacific R&D Center, Yongin 446-729, Republic of KoreaMedical Beauty Research Institute, AmorePacific R&D Center, Yongin 446-729, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Suwon 440-746, Republic of KoreaLutein is a naturally occurring carotenoid with antioxidative, antitumorigenic, antiangiogenic, photoprotective, hepatoprotective, and neuroprotective properties. Although the anti-inflammatory effects of lutein have previously been described, the mechanism of its anti-inflammatory action has not been fully elucidated. Therefore, in the present study, we aimed to investigate the regulatory activity of lutein in the inflammatory responses of skin-derived keratinocytes or macrophages and to elucidate the mechanism of its inhibitory action. Lutein significantly reduced several skin inflammatory responses, including increased expression of interleukin-(IL-) 6 from LPS-treated macrophages, upregulation of cyclooxygenase-(COX-) 2 from interferon-γ/tumor necrosis-factor-(TNF-) α-treated HaCaT cells, and the enhancement of matrix-metallopeptidase-(MMP-) 9 level in UV-irradiated keratinocytes. By evaluating the intracellular signaling pathway and the nuclear transcription factor levels, we determined that lutein inhibited the activation of redox-sensitive AP-1 pathway by suppressing the activation of p38 and c-Jun-N-terminal kinase (JNK). Evaluation of the radical and ROS scavenging activities further revealed that lutein was able to act as a strong anti-oxidant. Taken together, our findings strongly suggest that lutein-mediated AP-1 suppression and anti-inflammatory activity are the result of its strong antioxidative and p38/JNK inhibitory activities. These findings can be applied for the preparation of anti-inflammatory and cosmetic remedies for inflammatory diseases of the skin.http://dx.doi.org/10.1155/2013/787042 |
| spellingShingle | Jueun Oh Ji Hye Kim Jae Gwang Park Young-Su Yi Kye Won Park Ho Sik Rho Min-Seuk Lee Jae Won Yoo Seung-Hyun Kang Yong Deog Hong Song Seok Shin Jae Youl Cho Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells Mediators of Inflammation |
| title | Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells |
| title_full | Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells |
| title_fullStr | Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells |
| title_full_unstemmed | Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells |
| title_short | Radical Scavenging Activity-Based and AP-1-Targeted Anti-Inflammatory Effects of Lutein in Macrophage-Like and Skin Keratinocytic Cells |
| title_sort | radical scavenging activity based and ap 1 targeted anti inflammatory effects of lutein in macrophage like and skin keratinocytic cells |
| url | http://dx.doi.org/10.1155/2013/787042 |
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