Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus
Background: Type I diabetes mellitus (T1DM) is one of the most common chronic autoimmune diseases worldwide. miRNAs are a class of small non-coding RNA molecules that have been linked to immune system functions, β-cell metabolism, proliferation, and death, all of which contribute to pathogenesis of...
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054024001008 |
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| author | Amal A. Mohamed Gamil M. Abdallah Ibrahim T. Ibrahim Nada S. Ali Mona A. Hussein Ghada Maher Thabet Omar M. azzam Amira Yones Mohamed Maysa I. farghly Eman Al Hussain Samia S. Alkhalil Alaa Aly Mohamed Abouaggour Noheir Ashraf Ibrahem Fathy Hassan Seema Iqbal Ahmed Ali Mohamed Wael Hafez Mohamed O. Mahmoud |
| author_facet | Amal A. Mohamed Gamil M. Abdallah Ibrahim T. Ibrahim Nada S. Ali Mona A. Hussein Ghada Maher Thabet Omar M. azzam Amira Yones Mohamed Maysa I. farghly Eman Al Hussain Samia S. Alkhalil Alaa Aly Mohamed Abouaggour Noheir Ashraf Ibrahem Fathy Hassan Seema Iqbal Ahmed Ali Mohamed Wael Hafez Mohamed O. Mahmoud |
| author_sort | Amal A. Mohamed |
| collection | DOAJ |
| description | Background: Type I diabetes mellitus (T1DM) is one of the most common chronic autoimmune diseases worldwide. miRNAs are a class of small non-coding RNA molecules that have been linked to immune system functions, β-cell metabolism, proliferation, and death, all of which contribute to pathogenesis of TIDM. Dysregulated miRNAs have been identified in Egyptian TIDM patients. Aim: Several miRNAs were profiled in Egyptian TIDM patients to determine whether they can be used as molecular biomarkers for T1DM. The relationship between the investigated miRNAs and pro-inflammatory cytokines (TNF-α and IL-6) has also been evaluated in the development of TIDM, in addition to the creation of a proposed model for TIDM prediction. Patients & methods: Case-control study included 177 Egyptian patients with confirmed type I diabetes mellitus and 177 healthy individuals. MiRNA-34 and miRNA-146 were detected in serum samples using real-time PCR, whereas TNF-α and IL-6 levels were assessed using ELIZA. Results: Patients with TIDM showed a significant decrease in the expression of miRNA-146, with a cut-off value ≤ 3.3, 48 % specificity, and 92.1 % sensitivity, whereas miRNA-34 had the highest sensitivity (95.5 %) and specificity (97.2 %) for differentiating diabetic patients from controls. Furthermore, other diagnostic proinflammatory markers showed lower sensitivity and specificity. Conclusion: Serum levels of miRNA-34a, miRNA-146, IL-6, and TNF-α provide new insights into T1DM pathogenesis and could be used for screening and diagnosis purposes. They can be also a potential therapeutic target, as well as allowing for more strategies to improve T1DM disease outcomes. |
| format | Article |
| id | doaj-art-9b22d46aac1641a0b415b6022b5a821e |
| institution | Kabale University |
| issn | 2468-0540 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj-art-9b22d46aac1641a0b415b6022b5a821e2024-12-13T11:00:22ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402024-12-019412491256Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitusAmal A. Mohamed0Gamil M. Abdallah1Ibrahim T. Ibrahim2Nada S. Ali3Mona A. Hussein4Ghada Maher Thabet5Omar M. azzam6Amira Yones Mohamed7Maysa I. farghly8Eman Al Hussain9Samia S. Alkhalil10Alaa Aly Mohamed Abouaggour11Noheir Ashraf Ibrahem Fathy Hassan12Seema Iqbal13Ahmed Ali Mohamed14Wael Hafez15Mohamed O. Mahmoud16Biochemistry and Molecular Biology Department, National Hepatology and Tropical Medicine Research Institute, GOTHI, Cairo, EgyptBiochemistry Department, Faculty of Pharmacy, Egyptian Russian University, Cairo, EgyptBiochemistry Department, Faculty of Pharmacy, Beni Suef University, Beni Suef, EgyptBiochemistry Department, Faculty of Pharmacy, Egyptian Russian University, Cairo, EgyptInternal Medicine Department, National Institute of Diabetes and Endocrinology, GOTHI, Cairo, EgyptClinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, EgyptInternal Medicine Department, Ahmed Maher Teaching Hospital, GOTHI, Cairo, EgyptInternal medicine department, ELmatareya Teaching Hospital, GOTHI, Cairo, EgyptDepartment of Clinical Pathology, Faculty of Medicine, Suez University, Suez, EgyptClinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, EgyptDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Alquwayiyah, Riyadh, Saudi ArabiaMediclinic Hospital, Abu Dhabi, United Arab EmiratesFaculty of Medicine, Aswan University, Aswan, EgyptKhyber Medical College, Peshawar, PakistanIntensive Care Department, Theodor Bilharz Institute, Giza, EgyptInternal Medicine Department, Medical Research and Clinical Studies Institute, The National Research Centre, 33 El Buhouth St, Ad Doqi, Dokki, Cairo Governorate 12622, Egypt; Corresponding author. Internal Medicine Department; The National Research Centre, 33 El Buhouth St, Ad Doqi, Dokki, Cairo Governorate 12622, Egypt, Cairo, Egypt.Biochemistry Department, Faculty of Pharmacy, Beni Suef University, Beni Suef, EgyptBackground: Type I diabetes mellitus (T1DM) is one of the most common chronic autoimmune diseases worldwide. miRNAs are a class of small non-coding RNA molecules that have been linked to immune system functions, β-cell metabolism, proliferation, and death, all of which contribute to pathogenesis of TIDM. Dysregulated miRNAs have been identified in Egyptian TIDM patients. Aim: Several miRNAs were profiled in Egyptian TIDM patients to determine whether they can be used as molecular biomarkers for T1DM. The relationship between the investigated miRNAs and pro-inflammatory cytokines (TNF-α and IL-6) has also been evaluated in the development of TIDM, in addition to the creation of a proposed model for TIDM prediction. Patients & methods: Case-control study included 177 Egyptian patients with confirmed type I diabetes mellitus and 177 healthy individuals. MiRNA-34 and miRNA-146 were detected in serum samples using real-time PCR, whereas TNF-α and IL-6 levels were assessed using ELIZA. Results: Patients with TIDM showed a significant decrease in the expression of miRNA-146, with a cut-off value ≤ 3.3, 48 % specificity, and 92.1 % sensitivity, whereas miRNA-34 had the highest sensitivity (95.5 %) and specificity (97.2 %) for differentiating diabetic patients from controls. Furthermore, other diagnostic proinflammatory markers showed lower sensitivity and specificity. Conclusion: Serum levels of miRNA-34a, miRNA-146, IL-6, and TNF-α provide new insights into T1DM pathogenesis and could be used for screening and diagnosis purposes. They can be also a potential therapeutic target, as well as allowing for more strategies to improve T1DM disease outcomes.http://www.sciencedirect.com/science/article/pii/S2468054024001008TIDMPro-inflammatory cytokinesIL-6TNF-αmiRNA-34miRNA-14 |
| spellingShingle | Amal A. Mohamed Gamil M. Abdallah Ibrahim T. Ibrahim Nada S. Ali Mona A. Hussein Ghada Maher Thabet Omar M. azzam Amira Yones Mohamed Maysa I. farghly Eman Al Hussain Samia S. Alkhalil Alaa Aly Mohamed Abouaggour Noheir Ashraf Ibrahem Fathy Hassan Seema Iqbal Ahmed Ali Mohamed Wael Hafez Mohamed O. Mahmoud Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus Non-coding RNA Research TIDM Pro-inflammatory cytokines IL-6 TNF-α miRNA-34 miRNA-14 |
| title | Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus |
| title_full | Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus |
| title_fullStr | Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus |
| title_full_unstemmed | Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus |
| title_short | Evaluation of miRNA-146a, miRNA-34a, and pro-inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus |
| title_sort | evaluation of mirna 146a mirna 34a and pro inflammatory cytokines as a potential early indicators for type 1 diabetes mellitus |
| topic | TIDM Pro-inflammatory cytokines IL-6 TNF-α miRNA-34 miRNA-14 |
| url | http://www.sciencedirect.com/science/article/pii/S2468054024001008 |
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