Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep
Mucin-1 (MUC-1) is an anti-adhesion glycoprotein expressed on the apical surface of the endometrial luminal and glandular epithelium which prevents interactions between the trophectoderm and endometrial epithelium. The aim of this study was to investigate the outcome of PG-600 on ovine endometrial M...
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University of Basrah
2024-12-01
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| Series: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
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| Online Access: | https://www.bjas.bajas.edu.iq/index.php/bjas/article/view/2005 |
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| author | Hayder M.H. Habeeb Ann Ramsey Michelle A. Kutzler |
| author_facet | Hayder M.H. Habeeb Ann Ramsey Michelle A. Kutzler |
| author_sort | Hayder M.H. Habeeb |
| collection | DOAJ |
| description | Mucin-1 (MUC-1) is an anti-adhesion glycoprotein expressed on the apical surface of the endometrial luminal and glandular epithelium which prevents interactions between the trophectoderm and endometrial epithelium. The aim of this study was to investigate the outcome of PG-600 on ovine endometrial MUC-1 immunoexpression during early and mid-diestrus. Twenty-four cycling ewes (Polypay) were administered progesterone for 9 days. Two days before progesterone withdrawal, ewes were treated with cloprostenol. At progesterone withdrawal (d0), ewes were treated with either PG-600 (5 mL) or saline (5 mL) group. Endometrial tissues were collected surgically on days 4 and 7 after PG-600 or saline (n=6/group/timepoint) and were subjected to immunohistochemistry followed by image acquisition. Stained area for MUC-1 was quantified using Image J. The diestrous intercaruncular endometrium of both treated and control ewes expressed MUC-1. However, the percent area expressing MUC-1 varied between endometrial area (luminal and superficial glandular epithelium), time (day four, day 7), and treatment group. PG-600 treatment increased MUC-1 expression in the luminal epithelium on day 7 and in the superficial glandular epithelium expression on day 4. The results of this study show that administration of PG-600 to ewes increases endometrial MUC-1 expression in mid diestrus, which provides a possible mechanism for lower fertility found following treatment by PG-600 in sheep.
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| format | Article |
| id | doaj-art-9aea04f7720740f89b97a22cda44d815 |
| institution | Kabale University |
| issn | 1814-5868 2520-0860 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | University of Basrah |
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| series | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| spelling | doaj-art-9aea04f7720740f89b97a22cda44d8152025-01-03T18:58:03ZengUniversity of BasrahMaǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ1814-58682520-08602024-12-01372Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheepHayder M.H. Habeeb0Ann Ramsey1Michelle A. Kutzler2Department of Animal Production, College of Agriculture, Al-Qasim Green University, Babylon, Iraq 51001Carlson College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, USA 97331Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, Oregon, USA 97331Mucin-1 (MUC-1) is an anti-adhesion glycoprotein expressed on the apical surface of the endometrial luminal and glandular epithelium which prevents interactions between the trophectoderm and endometrial epithelium. The aim of this study was to investigate the outcome of PG-600 on ovine endometrial MUC-1 immunoexpression during early and mid-diestrus. Twenty-four cycling ewes (Polypay) were administered progesterone for 9 days. Two days before progesterone withdrawal, ewes were treated with cloprostenol. At progesterone withdrawal (d0), ewes were treated with either PG-600 (5 mL) or saline (5 mL) group. Endometrial tissues were collected surgically on days 4 and 7 after PG-600 or saline (n=6/group/timepoint) and were subjected to immunohistochemistry followed by image acquisition. Stained area for MUC-1 was quantified using Image J. The diestrous intercaruncular endometrium of both treated and control ewes expressed MUC-1. However, the percent area expressing MUC-1 varied between endometrial area (luminal and superficial glandular epithelium), time (day four, day 7), and treatment group. PG-600 treatment increased MUC-1 expression in the luminal epithelium on day 7 and in the superficial glandular epithelium expression on day 4. The results of this study show that administration of PG-600 to ewes increases endometrial MUC-1 expression in mid diestrus, which provides a possible mechanism for lower fertility found following treatment by PG-600 in sheep. https://www.bjas.bajas.edu.iq/index.php/bjas/article/view/2005EndometriumIimmunohistochemistryluminal epitheliumPregnancy lossSheep |
| spellingShingle | Hayder M.H. Habeeb Ann Ramsey Michelle A. Kutzler Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ Endometrium Iimmunohistochemistry luminal epithelium Pregnancy loss Sheep |
| title | Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep |
| title_full | Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep |
| title_fullStr | Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep |
| title_full_unstemmed | Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep |
| title_short | Endometrial Mucin-1 Expression Increases In Mid-Diestrus Following PG-600 Administration in sheep |
| title_sort | endometrial mucin 1 expression increases in mid diestrus following pg 600 administration in sheep |
| topic | Endometrium Iimmunohistochemistry luminal epithelium Pregnancy loss Sheep |
| url | https://www.bjas.bajas.edu.iq/index.php/bjas/article/view/2005 |
| work_keys_str_mv | AT haydermhhabeeb endometrialmucin1expressionincreasesinmiddiestrusfollowingpg600administrationinsheep AT annramsey endometrialmucin1expressionincreasesinmiddiestrusfollowingpg600administrationinsheep AT michelleakutzler endometrialmucin1expressionincreasesinmiddiestrusfollowingpg600administrationinsheep |