RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts
Somatic nuclear transfer technology has become increasingly promising in biomedicine and agriculture. Whereas the approach remains inefficient and underlying mechanisms remain ambiguous. Although cloned embryos have similar in vitro developmental capacity as in vitro fertilized (IVF) embryos before...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2015-05-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311914608662 |
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| author | Wei-hua XU Zi-cong LI Zhi-ping OUYANG Bo YU Jun-song SHI De-wu LIU Zhen-fang WU |
| author_facet | Wei-hua XU Zi-cong LI Zhi-ping OUYANG Bo YU Jun-song SHI De-wu LIU Zhen-fang WU |
| author_sort | Wei-hua XU |
| collection | DOAJ |
| description | Somatic nuclear transfer technology has become increasingly promising in biomedicine and agriculture. Whereas the approach remains inefficient and underlying mechanisms remain ambiguous. Although cloned embryos have similar in vitro developmental capacity as in vitro fertilized (IVF) embryos before implantation, they appeared to have much lower full-term developmental efficiency in pig and cattle, and thus it would be reasonable to postulate that profound distinction at the molecular level should exist between them. Herein, RNA sequencing technique was used to screen differentially expressed genes in cloned and IVF blastocysts, and in total 628 differentially expressed transcripts were obtained, among which, 280 transcripts are up-regulated and 348 transcripts are down-regulated in cloned blastocysts. Moreover, one statistically significant pathway associated with endoplasmic reticulum (ER) protein processing was enriched, and some ER-stress markers such as ATF4, ATF6, PDIA3, HSPA1B, HSP40 and HSP90 between cloned and IVF blastocysts were suggested. Additionally, some developmentally important genes such as lipid metabolism related genes (MGLL, DDHD2 and FADS2) and epigenetic modification genes (DNMT1, KDM5C and MBD3L5) were found differentially expressed between cloned and IVF embryos. |
| format | Article |
| id | doaj-art-9ab09aee1bac4d4a962a17ac7dec3616 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-9ab09aee1bac4d4a962a17ac7dec36162025-08-20T03:57:08ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192015-05-0114592693810.1016/S2095-3119(14)60866-2RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocystsWei-hua XU0Zi-cong LI1Zhi-ping OUYANG2Bo YU3Jun-song SHI4De-wu LIU5Zhen-fang WU6National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, P.R. China; College of Life Science, Longyan University, Longyan 364012, P.R. China; XU Wei-hua, Mobile: 13859594289National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, P.R. ChinaWen's Research Institute, Yunfu 527439, P.R. ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, P.R. ChinaNational Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, P.R. China; Wen's Research Institute, Yunfu 527439, P.R. China; Correspondence WU Zhen-fang, Tel/Fax: +86-20-85280369Somatic nuclear transfer technology has become increasingly promising in biomedicine and agriculture. Whereas the approach remains inefficient and underlying mechanisms remain ambiguous. Although cloned embryos have similar in vitro developmental capacity as in vitro fertilized (IVF) embryos before implantation, they appeared to have much lower full-term developmental efficiency in pig and cattle, and thus it would be reasonable to postulate that profound distinction at the molecular level should exist between them. Herein, RNA sequencing technique was used to screen differentially expressed genes in cloned and IVF blastocysts, and in total 628 differentially expressed transcripts were obtained, among which, 280 transcripts are up-regulated and 348 transcripts are down-regulated in cloned blastocysts. Moreover, one statistically significant pathway associated with endoplasmic reticulum (ER) protein processing was enriched, and some ER-stress markers such as ATF4, ATF6, PDIA3, HSPA1B, HSP40 and HSP90 between cloned and IVF blastocysts were suggested. Additionally, some developmentally important genes such as lipid metabolism related genes (MGLL, DDHD2 and FADS2) and epigenetic modification genes (DNMT1, KDM5C and MBD3L5) were found differentially expressed between cloned and IVF embryos.http://www.sciencedirect.com/science/article/pii/S2095311914608662porcinetranscriptomeblastocystsomatic cell nuclear transferin vitro fertilization |
| spellingShingle | Wei-hua XU Zi-cong LI Zhi-ping OUYANG Bo YU Jun-song SHI De-wu LIU Zhen-fang WU RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts Journal of Integrative Agriculture porcine transcriptome blastocyst somatic cell nuclear transfer in vitro fertilization |
| title | RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts |
| title_full | RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts |
| title_fullStr | RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts |
| title_full_unstemmed | RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts |
| title_short | RNA-Seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts |
| title_sort | rna seq transcriptome analysis of porcine cloned and in vitro fertilized blastocysts |
| topic | porcine transcriptome blastocyst somatic cell nuclear transfer in vitro fertilization |
| url | http://www.sciencedirect.com/science/article/pii/S2095311914608662 |
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