An optimized QIAzol-based protocol for simultaneous miRNA, RNA, and protein isolation from precision-cut lung slices (PCLS)

An optimized QIAzol-based protocol for simultaneous miRNA, RNA, and protein isolation from precision-cut lung slices (PCLS)

Abstract Background Precision-cut lung slices (PCLS) are ex vivo models with preserved lung cell populations and maintained tissue architecture. PCLS are, therefore, a powerful tool in respiratory research to study molecular mechanisms that closely reflect whole tissue biology. High-quality RNA and...

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Bibliographic Details
Main Authors: Wojciech Langwiński, Joanna Nowakowska, Kosma Sakrajda, Kamil Ziarniak, Zuzanna Stachowiak, Maria Kachel, Beata Narożna, Aleksandra Szczepankiewicz
Format: Article
Language:English
Published: BMC 2024-11-01
Series:Respiratory Research
Subjects:
Extraction yield
QIAzol
miRNA
RNA
Protein
Online Access:https://doi.org/10.1186/s12931-024-03026-3
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