Platelet-Rich Plasma Inhibits Breast Cancer Proliferation
Background: Platelet-rich plasma (PRP) helps promote wound healing, but it is unclear whether it stimulates breast cancer cell proliferation, which restricts its application in breast cancer patients. This article explored the effect of PRP on breast cancer cell proliferation through preclinical exp...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2024-11-01
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| Series: | Clinical Medicine Insights: Oncology |
| Online Access: | https://doi.org/10.1177/11795549241298978 |
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| _version_ | 1846165994907631616 |
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| author | Chao Han Caiping Chen Ning Lu Li Xue Dan Xing Wanxin Wu Wang Li Xiang Lu |
| author_facet | Chao Han Caiping Chen Ning Lu Li Xue Dan Xing Wanxin Wu Wang Li Xiang Lu |
| author_sort | Chao Han |
| collection | DOAJ |
| description | Background: Platelet-rich plasma (PRP) helps promote wound healing, but it is unclear whether it stimulates breast cancer cell proliferation, which restricts its application in breast cancer patients. This article explored the effect of PRP on breast cancer cell proliferation through preclinical experiments. Method: We cultivated MDA-MB-231 breast cancer cells with PRP in vitro. Subsequently, we employed Cell Counting Kit-8 (CCK-8) assays to assess their proliferation ability, wound healing assays to evaluate their migration ability, and Transwell assays to detect their invasion ability. Mouse breast cancer 4T1 cells were subcutaneously inoculated into nude mice. After tumor formation, PRP was injected around each tumor. Tumor size was measured. After 15 days, the tumors were surgically removed. Immunohistochemistry was used to detect key proteins involved in proliferation and apoptosis. Results: PRP inhibited the proliferation, migration, and invasion of MDA-MB-231 in vitro. After PRP was injected around tumors in nude mice, tumor growth slowed, Ki67 and phospho-histone H3 (pHH3) expression decreased, and Caspase 3 and Poly (adenosine diphosphate-ribose) polymerase 1 (PARP1) expression increased. Conclusion: The PRP inhibits the proliferation of breast cancer MDA-MB-231 and 4T1 cells. |
| format | Article |
| id | doaj-art-9a4e75b6eeef421db42de8b3c59db854 |
| institution | Kabale University |
| issn | 1179-5549 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Clinical Medicine Insights: Oncology |
| spelling | doaj-art-9a4e75b6eeef421db42de8b3c59db8542024-11-16T12:03:21ZengSAGE PublishingClinical Medicine Insights: Oncology1179-55492024-11-011810.1177/11795549241298978Platelet-Rich Plasma Inhibits Breast Cancer ProliferationChao Han0Caiping Chen1Ning Lu2Li Xue3Dan Xing4Wanxin Wu5Wang Li6Xiang Lu7Department of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Pathology, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Pathology, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaDepartment of Breast Surgery, Affiliated Hospital of Jiaxing University (The First Hospital of Jiaxing), Jiaxing, ChinaBackground: Platelet-rich plasma (PRP) helps promote wound healing, but it is unclear whether it stimulates breast cancer cell proliferation, which restricts its application in breast cancer patients. This article explored the effect of PRP on breast cancer cell proliferation through preclinical experiments. Method: We cultivated MDA-MB-231 breast cancer cells with PRP in vitro. Subsequently, we employed Cell Counting Kit-8 (CCK-8) assays to assess their proliferation ability, wound healing assays to evaluate their migration ability, and Transwell assays to detect their invasion ability. Mouse breast cancer 4T1 cells were subcutaneously inoculated into nude mice. After tumor formation, PRP was injected around each tumor. Tumor size was measured. After 15 days, the tumors were surgically removed. Immunohistochemistry was used to detect key proteins involved in proliferation and apoptosis. Results: PRP inhibited the proliferation, migration, and invasion of MDA-MB-231 in vitro. After PRP was injected around tumors in nude mice, tumor growth slowed, Ki67 and phospho-histone H3 (pHH3) expression decreased, and Caspase 3 and Poly (adenosine diphosphate-ribose) polymerase 1 (PARP1) expression increased. Conclusion: The PRP inhibits the proliferation of breast cancer MDA-MB-231 and 4T1 cells.https://doi.org/10.1177/11795549241298978 |
| spellingShingle | Chao Han Caiping Chen Ning Lu Li Xue Dan Xing Wanxin Wu Wang Li Xiang Lu Platelet-Rich Plasma Inhibits Breast Cancer Proliferation Clinical Medicine Insights: Oncology |
| title | Platelet-Rich Plasma Inhibits Breast Cancer Proliferation |
| title_full | Platelet-Rich Plasma Inhibits Breast Cancer Proliferation |
| title_fullStr | Platelet-Rich Plasma Inhibits Breast Cancer Proliferation |
| title_full_unstemmed | Platelet-Rich Plasma Inhibits Breast Cancer Proliferation |
| title_short | Platelet-Rich Plasma Inhibits Breast Cancer Proliferation |
| title_sort | platelet rich plasma inhibits breast cancer proliferation |
| url | https://doi.org/10.1177/11795549241298978 |
| work_keys_str_mv | AT chaohan plateletrichplasmainhibitsbreastcancerproliferation AT caipingchen plateletrichplasmainhibitsbreastcancerproliferation AT ninglu plateletrichplasmainhibitsbreastcancerproliferation AT lixue plateletrichplasmainhibitsbreastcancerproliferation AT danxing plateletrichplasmainhibitsbreastcancerproliferation AT wanxinwu plateletrichplasmainhibitsbreastcancerproliferation AT wangli plateletrichplasmainhibitsbreastcancerproliferation AT xianglu plateletrichplasmainhibitsbreastcancerproliferation |