A critical systematic review of extracellular vesicle clinical trials

Abstract This systematic review examines the landscape of extracellular vesicle (EV)‐related clinical trials to elucidate the field's trends in clinical applications and EV‐related methodologies, with an additional focus on the acknowledgement of EV subpopulations. By analysing data from public...

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Main Authors: Rachel R. Mizenko, Madison Feaver, Batuhan T. Bozkurt, Neona Lowe, Bryan Nguyen, Kuan‐Wei Huang, Aijun Wang, Randy P. Carney
Format: Article
Language:English
Published: Wiley 2024-10-01
Series:Journal of Extracellular Vesicles
Subjects:
Online Access:https://doi.org/10.1002/jev2.12510
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author Rachel R. Mizenko
Madison Feaver
Batuhan T. Bozkurt
Neona Lowe
Bryan Nguyen
Kuan‐Wei Huang
Aijun Wang
Randy P. Carney
author_facet Rachel R. Mizenko
Madison Feaver
Batuhan T. Bozkurt
Neona Lowe
Bryan Nguyen
Kuan‐Wei Huang
Aijun Wang
Randy P. Carney
author_sort Rachel R. Mizenko
collection DOAJ
description Abstract This systematic review examines the landscape of extracellular vesicle (EV)‐related clinical trials to elucidate the field's trends in clinical applications and EV‐related methodologies, with an additional focus on the acknowledgement of EV subpopulations. By analysing data from public reporting repositories, we catalogued 471 EV‐related clinical trials to date, with indications for over 200 diseases. Diagnostics and companion diagnostics represented the bulk of EV‐related clinical trials with cancer being the most frequent application. EV‐related therapeutics trials mainly utilized mesenchymal stromal cell (MSC) EVs and were most frequently used for treatment of respiratory illnesses. Ultracentrifugation and RNA‐sequencing were the most common isolation and characterization techniques; however, methodology for each was not frequently reported in study records. Most of the reported characterization relied on bulk characterization of EV isolates, with only 11% utilizing EV subpopulations in their experimental design. While this may be connected to a lack of available techniques suitable for clinical implementation, it also highlights the opportunity for use of EV subpopulations to improve translational efforts. As academic research identifies more chemically distinct subpopulations and technologies for their enrichment, we forecast to more refined EV trials in the near future. This review emphasizes the need for meticulous methodological reporting and consideration of EV subpopulations to enhance the translational success of EV‐based interventions, pointing towards a paradigm shift in personalized medicine.
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spelling doaj-art-9a3e8f2b68ae452097cfeb344d7646c52024-12-18T07:26:41ZengWileyJournal of Extracellular Vesicles2001-30782024-10-011310n/an/a10.1002/jev2.12510A critical systematic review of extracellular vesicle clinical trialsRachel R. Mizenko0Madison Feaver1Batuhan T. Bozkurt2Neona Lowe3Bryan Nguyen4Kuan‐Wei Huang5Aijun Wang6Randy P. Carney7Department of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USADepartment of Biomedical Engineering University of California Davis California USAAbstract This systematic review examines the landscape of extracellular vesicle (EV)‐related clinical trials to elucidate the field's trends in clinical applications and EV‐related methodologies, with an additional focus on the acknowledgement of EV subpopulations. By analysing data from public reporting repositories, we catalogued 471 EV‐related clinical trials to date, with indications for over 200 diseases. Diagnostics and companion diagnostics represented the bulk of EV‐related clinical trials with cancer being the most frequent application. EV‐related therapeutics trials mainly utilized mesenchymal stromal cell (MSC) EVs and were most frequently used for treatment of respiratory illnesses. Ultracentrifugation and RNA‐sequencing were the most common isolation and characterization techniques; however, methodology for each was not frequently reported in study records. Most of the reported characterization relied on bulk characterization of EV isolates, with only 11% utilizing EV subpopulations in their experimental design. While this may be connected to a lack of available techniques suitable for clinical implementation, it also highlights the opportunity for use of EV subpopulations to improve translational efforts. As academic research identifies more chemically distinct subpopulations and technologies for their enrichment, we forecast to more refined EV trials in the near future. This review emphasizes the need for meticulous methodological reporting and consideration of EV subpopulations to enhance the translational success of EV‐based interventions, pointing towards a paradigm shift in personalized medicine.https://doi.org/10.1002/jev2.12510diagnosticsdrug delivery vehiclesexosomesheterogeneitytherapeutics
spellingShingle Rachel R. Mizenko
Madison Feaver
Batuhan T. Bozkurt
Neona Lowe
Bryan Nguyen
Kuan‐Wei Huang
Aijun Wang
Randy P. Carney
A critical systematic review of extracellular vesicle clinical trials
Journal of Extracellular Vesicles
diagnostics
drug delivery vehicles
exosomes
heterogeneity
therapeutics
title A critical systematic review of extracellular vesicle clinical trials
title_full A critical systematic review of extracellular vesicle clinical trials
title_fullStr A critical systematic review of extracellular vesicle clinical trials
title_full_unstemmed A critical systematic review of extracellular vesicle clinical trials
title_short A critical systematic review of extracellular vesicle clinical trials
title_sort critical systematic review of extracellular vesicle clinical trials
topic diagnostics
drug delivery vehicles
exosomes
heterogeneity
therapeutics
url https://doi.org/10.1002/jev2.12510
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