Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage
<i>Background and Objectives:</i> Ischemia-reperfusion (I/R) injury is a process in which impaired perfusion is restored by restoring blood flow and tissue recirculation. Nanomedicine uses cutting-edge technologies that emerge from interdisciplinary influences. In the literature, there a...
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2024-12-01
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| author | Işın Güneş Ali Doğan Dursun Çağrı Özdemir Ayşegül Küçük Şaban Cem Sezen Mustafa Arslan Abdullah Özer |
| author_facet | Işın Güneş Ali Doğan Dursun Çağrı Özdemir Ayşegül Küçük Şaban Cem Sezen Mustafa Arslan Abdullah Özer |
| author_sort | Işın Güneş |
| collection | DOAJ |
| description | <i>Background and Objectives:</i> Ischemia-reperfusion (I/R) injury is a process in which impaired perfusion is restored by restoring blood flow and tissue recirculation. Nanomedicine uses cutting-edge technologies that emerge from interdisciplinary influences. In the literature, there are very few in vivo and in vitro studies on how cerium oxide (CeO<sub>2</sub>) affects systemic anti-inflammatory response and inflammation. Therefore, in our study, we aimed to investigate whether CeO<sub>2</sub> administration has a protective effect against myocardial I/R injury in the liver and kidneys. <i>Materials and Methods:</i> Twenty-four rats were randomly divided into four groups after obtaining approval from an ethics committee. A control (group C), cerium oxide (group CO), IR (group IR), and Cerium oxide-IR (CO-IR group) groups were formed. Intraperitoneal CeO<sub>2</sub> was administered at a dose of 0.5 mg/kg 30 min before left thoracotomy and left main coronary (LAD) ligation, and myocardial muscle ischemia was induced for 30 min. After LAD ligation was removed, reperfusion was performed for 120 min. All rats were euthanized using ketamine, and blood was collected. Liver and kidney tissue samples were evaluated histopathologically. Serum AST (aspartate aminotransferase), ALT (alanine aminotransaminase), GGT (gamma-glutamyl transferase), glucose, TOS (<i>Total Oxidant Status</i>), and TAS (<i>Total Antioxidant Status</i>) levels were also measured. <i>Results:</i> Necrotic cell and mononuclear cell infiltration in the liver parenchyma of rats in the IR group was observed to be significantly increased compared to the other groups. Hepatocyte degeneration was greater in the IR group compared to groups C and CO. Vascular vacuolization and hypertrophy, tubular degeneration, and necrosis were increased in the kidney tissue of the IR group compared to the other groups. Tubular dilatation was significantly higher in the IR group than in the C and CO groups. TOS was significantly higher in all groups than in the IR group (<i>p</i> < 0.0001, <i>p</i> < 0.0001, and <i>p</i> = 0.006, respectively). However, TAS level was lower in the IR group than in the other groups (<i>p</i> = 0.002, <i>p</i> = 0.020, and <i>p</i> = 0.031, respectively). Renal and liver histopathological findings decreased significantly in the CO-IR group compared to the IR group. A decrease in the TOS level and an increase in the TAS level were found compared to the IR group. The AST, ALT, GGT, and Glucose levels are shown. <i>Conclusions:</i> CeO<sub>2</sub> administered before ischemia-reperfusion reduced oxidative stress and ameliorated IR-induced damage in distant organs. We suggest that CeO<sub>2</sub> exerts protective effects in the myocardial IR model. |
| format | Article |
| id | doaj-art-9a2d23f4840b4bee84a9dc215eb4b20f |
| institution | Kabale University |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj-art-9a2d23f4840b4bee84a9dc215eb4b20f2024-12-27T14:38:57ZengMDPI AGMedicina1010-660X1648-91442024-12-016012204410.3390/medicina60122044Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ DamageIşın Güneş0Ali Doğan Dursun1Çağrı Özdemir2Ayşegül Küçük3Şaban Cem Sezen4Mustafa Arslan5Abdullah Özer6Department of Anesthesiology and Reanimation, Erciyes University Faculty of Medicine, Kayseri 38039, TurkeyDepartment of Physiology, Atılım University Faculty of Medicine, Ankara 06560, TurkeyDepartment of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara 06560, TurkeyDepartment of Physiology, Kutahya Health Sciences University Faculty of Medicine, Kutahya 43100, TurkeyDepartment of Histology and Embryology, Kırıkkale University Faculty of Medicine, Kırıkkale 71000, TurkeyDepartment of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara 06560, TurkeyDepartment Cardiovascular Surgery, Gazi University Faculty of Medicine, Ankara 06560, Turkey<i>Background and Objectives:</i> Ischemia-reperfusion (I/R) injury is a process in which impaired perfusion is restored by restoring blood flow and tissue recirculation. Nanomedicine uses cutting-edge technologies that emerge from interdisciplinary influences. In the literature, there are very few in vivo and in vitro studies on how cerium oxide (CeO<sub>2</sub>) affects systemic anti-inflammatory response and inflammation. Therefore, in our study, we aimed to investigate whether CeO<sub>2</sub> administration has a protective effect against myocardial I/R injury in the liver and kidneys. <i>Materials and Methods:</i> Twenty-four rats were randomly divided into four groups after obtaining approval from an ethics committee. A control (group C), cerium oxide (group CO), IR (group IR), and Cerium oxide-IR (CO-IR group) groups were formed. Intraperitoneal CeO<sub>2</sub> was administered at a dose of 0.5 mg/kg 30 min before left thoracotomy and left main coronary (LAD) ligation, and myocardial muscle ischemia was induced for 30 min. After LAD ligation was removed, reperfusion was performed for 120 min. All rats were euthanized using ketamine, and blood was collected. Liver and kidney tissue samples were evaluated histopathologically. Serum AST (aspartate aminotransferase), ALT (alanine aminotransaminase), GGT (gamma-glutamyl transferase), glucose, TOS (<i>Total Oxidant Status</i>), and TAS (<i>Total Antioxidant Status</i>) levels were also measured. <i>Results:</i> Necrotic cell and mononuclear cell infiltration in the liver parenchyma of rats in the IR group was observed to be significantly increased compared to the other groups. Hepatocyte degeneration was greater in the IR group compared to groups C and CO. Vascular vacuolization and hypertrophy, tubular degeneration, and necrosis were increased in the kidney tissue of the IR group compared to the other groups. Tubular dilatation was significantly higher in the IR group than in the C and CO groups. TOS was significantly higher in all groups than in the IR group (<i>p</i> < 0.0001, <i>p</i> < 0.0001, and <i>p</i> = 0.006, respectively). However, TAS level was lower in the IR group than in the other groups (<i>p</i> = 0.002, <i>p</i> = 0.020, and <i>p</i> = 0.031, respectively). Renal and liver histopathological findings decreased significantly in the CO-IR group compared to the IR group. A decrease in the TOS level and an increase in the TAS level were found compared to the IR group. The AST, ALT, GGT, and Glucose levels are shown. <i>Conclusions:</i> CeO<sub>2</sub> administered before ischemia-reperfusion reduced oxidative stress and ameliorated IR-induced damage in distant organs. We suggest that CeO<sub>2</sub> exerts protective effects in the myocardial IR model.https://www.mdpi.com/1648-9144/60/12/2044myocardial ischemia reperfusioncerium oxidekidneyliverTOSTAS |
| spellingShingle | Işın Güneş Ali Doğan Dursun Çağrı Özdemir Ayşegül Küçük Şaban Cem Sezen Mustafa Arslan Abdullah Özer Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage Medicina myocardial ischemia reperfusion cerium oxide kidney liver TOS TAS |
| title | Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage |
| title_full | Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage |
| title_fullStr | Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage |
| title_full_unstemmed | Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage |
| title_short | Effects of Cerium Oxide on Kidney and Liver Tissue Damage in an Experimental Myocardial Ischemia-Reperfusion Model of Distant Organ Damage |
| title_sort | effects of cerium oxide on kidney and liver tissue damage in an experimental myocardial ischemia reperfusion model of distant organ damage |
| topic | myocardial ischemia reperfusion cerium oxide kidney liver TOS TAS |
| url | https://www.mdpi.com/1648-9144/60/12/2044 |
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