Isolation and transcriptional profiling of antigen-presenting cells from mouse lung and MLNs following intranasal immunization

Isolation and transcriptional profiling of antigen-presenting cells from mouse lung and MLNs following intranasal immunization

Summary: Dendritic cells (DCs) play a central role in the initiation of the adaptive immune response. Here, we present a protocol for isolating and transcriptionally profiling antigen-presenting cells (APCs) from the mouse lung and mediastinal lymph nodes (MLNs) following intranasal immunization. We...

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Bibliographic Details
Main Authors: Xiuyu Wang, Yihan Wang, Qiang Wang, Anita S. Chong, Shengbo Cao, Youhui Si
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Flow Cytometry
RNA-Seq
Immunology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005653
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Summary:Summary: Dendritic cells (DCs) play a central role in the initiation of the adaptive immune response. Here, we present a protocol for isolating and transcriptionally profiling antigen-presenting cells (APCs) from the mouse lung and mediastinal lymph nodes (MLNs) following intranasal immunization. We describe steps for preparing single-cell suspensions from the lung and MLN, along with the detection and RNA sequencing (RNA-seq) of antigen-presenting DCs. This protocol offers a broadly applicable approach for identifying variations in DC subpopulations under diverse experimental conditions.For complete details on the use and execution of this protocol, please refer to Youhui et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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