Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)

Abstract The maize weevil, Sitophilus zeamais Motschulsky, is one of the most destructive pests of stored grains worldwide, posing a significant threat to global food security. To better understand the biology, resistance mechanism, and adaptive evolution of this species, we presented a high-quality...

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Main Authors: Yueliang Bai, Fangfang Zeng, Meng Zhang, Chao Zhao, Shuai Pang, Guiyao Wang
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05341-w
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author Yueliang Bai
Fangfang Zeng
Meng Zhang
Chao Zhao
Shuai Pang
Guiyao Wang
author_facet Yueliang Bai
Fangfang Zeng
Meng Zhang
Chao Zhao
Shuai Pang
Guiyao Wang
author_sort Yueliang Bai
collection DOAJ
description Abstract The maize weevil, Sitophilus zeamais Motschulsky, is one of the most destructive pests of stored grains worldwide, posing a significant threat to global food security. To better understand the biology, resistance mechanism, and adaptive evolution of this species, we presented a high-quality chromosome-level genome assembly of S. zeamais using PacBio sequencing and Hi-C technologies. The size of the final assembled genome was 693.21 Mb with scaffold N50 of 61.03 Mb, and 631.97 Mb were successfully anchored into 11 pseudochromosomes. In total, 15,161 protein-coding genes were annotated, of which 98.89% obtained functional descriptions. Additionally, 377.50 Mb of sequences were identified as repeat elements, accounting for 54.46% of the genome. BUSCO analysis revealed a high level of completeness in both the genome assembly and annotation, with scores of 98.17% and 97.22%, respectively. The chromosome-level genome of S. zeamais provides valuable genomic insights that deepen our understanding of the evolution and ecology of Sitophilus species, while also contributing to the development of targeted and innovative control strategies for stored-product pests.
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id doaj-art-99e4f8af5d6c4e5eac005f80e7473df9
institution Kabale University
issn 2052-4463
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publishDate 2025-06-01
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spelling doaj-art-99e4f8af5d6c4e5eac005f80e7473df92025-08-20T03:45:11ZengNature PortfolioScientific Data2052-44632025-06-011211710.1038/s41597-025-05341-wChromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)Yueliang Bai0Fangfang Zeng1Meng Zhang2Chao Zhao3Shuai Pang4Guiyao Wang5Henan Collaborative Innovation Center for Grain Storage Security, School of Food and Strategic Reserves, Henan University of TechnologyHenan Collaborative Innovation Center for Grain Storage Security, School of Food and Strategic Reserves, Henan University of TechnologyHenan Collaborative Innovation Center for Grain Storage Security, School of Food and Strategic Reserves, Henan University of TechnologyHenan Collaborative Innovation Center for Grain Storage Security, School of Food and Strategic Reserves, Henan University of TechnologyGlbizzia BiosciencesZhengzhou Tobacco Research Institute of CNTCAbstract The maize weevil, Sitophilus zeamais Motschulsky, is one of the most destructive pests of stored grains worldwide, posing a significant threat to global food security. To better understand the biology, resistance mechanism, and adaptive evolution of this species, we presented a high-quality chromosome-level genome assembly of S. zeamais using PacBio sequencing and Hi-C technologies. The size of the final assembled genome was 693.21 Mb with scaffold N50 of 61.03 Mb, and 631.97 Mb were successfully anchored into 11 pseudochromosomes. In total, 15,161 protein-coding genes were annotated, of which 98.89% obtained functional descriptions. Additionally, 377.50 Mb of sequences were identified as repeat elements, accounting for 54.46% of the genome. BUSCO analysis revealed a high level of completeness in both the genome assembly and annotation, with scores of 98.17% and 97.22%, respectively. The chromosome-level genome of S. zeamais provides valuable genomic insights that deepen our understanding of the evolution and ecology of Sitophilus species, while also contributing to the development of targeted and innovative control strategies for stored-product pests.https://doi.org/10.1038/s41597-025-05341-w
spellingShingle Yueliang Bai
Fangfang Zeng
Meng Zhang
Chao Zhao
Shuai Pang
Guiyao Wang
Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)
Scientific Data
title Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)
title_full Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)
title_fullStr Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)
title_full_unstemmed Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)
title_short Chromosome-level genome assembly and annotation of the maize weevil (Sitophilus zeamais Motschulsky)
title_sort chromosome level genome assembly and annotation of the maize weevil sitophilus zeamais motschulsky
url https://doi.org/10.1038/s41597-025-05341-w
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