Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

Abstract To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary. The human cytomegalovirus (CMV) promoter is widely used for RTP expression in CHO cells. To further improve RTP pro...

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Bibliographic Details
Main Authors: Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
Recombinant therapeutic protein
Chinese hamster ovary cells
Expression vector
Promoter
Intron
Online Access:https://doi.org/10.1038/s41598-025-87941-5
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