Influence of <i>Pediococcus acidilactici</i> and <i>Bacillus coagulans</i> on In Vitro Ruminal Greenhouse Gas Production of Fermented Devilfish in Livestock Rumen Contents
This study aimed to evaluate the effect of including silage from devilfish waste (SF-<i>Hypostomus plecostomus</i>) and probiotics (PB-<i>Pediococcus acidilactici</i> BX-B122 and <i>Bacillus coagulans</i> BX-B118) in ruminants on greenhouse gas production. The die...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/7/416 |
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| Summary: | This study aimed to evaluate the effect of including silage from devilfish waste (SF-<i>Hypostomus plecostomus</i>) and probiotics (PB-<i>Pediococcus acidilactici</i> BX-B122 and <i>Bacillus coagulans</i> BX-B118) in ruminants on greenhouse gas production. The diets evaluated contained 0, 8, 14 and 20% of silage made from SF and the addition of PB at a dose of 0.2 mL/g of diet, using steers and sheep (rams) as rumen inoculum donors in a completely randomized statistical design with a 2 × 4 × 2 factorial arrangement. Asymptotic gas production (GP) was influenced (<i>p</i> < 0.01) by the interactions between rumen liquor (RL), SF, and PB. The inclusion of SF and PB resulted in a higher (<i>p</i> < 0.01) GP rate in sheep; however, the values were reduced with increasing levels of SF. Asymptotic CH<sub>4</sub> in the rumen fluid of steers decreased with an increasing SF percentage up to 14%. Probiotics had different effects on the rumen fluid of sheep and steers. In steers, probiotics substantially reduced (<i>p</i> < 0.01) CH<sub>4</sub> synthesis while supplementation increased it in sheep rumen fluid. Similarly, diets with probiotics had higher CO formation (<i>p</i> < 0.05) in sheep and steer liquor. Similarly, CO decreased (<i>p</i> < 0.05) with increasing levels of SF. In the rumen fluid of sheep and steers, the probiotics were found to reduce H<sub>2</sub>S, while there was an SF-dose-dependent decrease in H<sub>2</sub>S concentration. The ruminal pH and dry matter digestibility of sheep were higher than in steers. It can be concluded that increasing SF levels generally reduced the total gas and CH<sub>4</sub> production, with probiotics further enhancing this reduction, especially in CH<sub>4</sub> per unit of gas. |
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| ISSN: | 2311-5637 |