Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer
Relevance. Colorectal cancer (CRC) is the second leading cause of cancer mortality worldwide. Non-invasive diagnostic methods based on the determination of hidden blood in the stool (fecal immunochemical test, guaiac test), which have been proven to be effective in clinical studies, are used for CRC...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2024-11-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/18688/1523 |
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| author | Viktoriya N. Shumilova Artemij E. Goncharov Daniil V. Azarov Stanislav I. Sitkin Elgudzha L. Latariya Batyrbek I. Aslanov Mikhail A. Bobrakov Rustem E. Topuzov |
| author_facet | Viktoriya N. Shumilova Artemij E. Goncharov Daniil V. Azarov Stanislav I. Sitkin Elgudzha L. Latariya Batyrbek I. Aslanov Mikhail A. Bobrakov Rustem E. Topuzov |
| author_sort | Viktoriya N. Shumilova |
| collection | DOAJ |
| description | Relevance. Colorectal cancer (CRC) is the second leading cause of cancer mortality worldwide. Non-invasive diagnostic methods based on the determination of hidden blood in the stool (fecal immunochemical test, guaiac test), which have been proven to be effective in clinical studies, are used for CRC screening. However, a significant disadvantage of the available non-invasive diagnostic methods is the low sensitivity in detecting the oncological process at the early stages. A number of recent studies discuss the relationship between the disease and various potentially oncogenic microorganisms in the human intestinal tract, which can be used to expand the arsenal of non-invasive methods for diagnosing CRC based on molecular genetic examination of a stool sample to identify oncogenic microorganisms.
The aim of this study was to evaluate the possibility of using genetic determinants of potentially oncogenic microorganisms as markers for colorectal cancer, based on a comparison of their prevalence in groups of patients with colorectal cancer, facultative precancerous diseases and patients without intestinal pathology.
Materials and methods. 215 participants were included in the "case–control" study: 70 patients with newly diagnosed colorectal cancer, 70 patients with inflammatory bowel disease, 75 participants without diagnosed intestinal pathology. Polymerase chain reaction (PCR) was used to identify and detect genes of potentially oncogenic microorganisms.
Results and discussion. An association was found between CRC and the presence of the Bacteroides fragilis fragilisin gene (OR 7.00; 95% CI: 2.55–22.50; p 0.001), species-specific genes of the periodontal pathogenic microorganisms Fusobacterium nucleatum (OR 5.61; 95% CI: 2.87–11.30; p 0.001) and Porphyromonas gingivalis (OR 16.3; 95% CI: 4.33–106.00; p 0.001), the clbB gene of pks pathogenicity island of the Enterobacteria (OR 3.44; 95% CI: 1.39–8.51; p = 0.010).
Conclusion. The presence of genetic markers of potentially oncogenic bacterial species and genotypes in the gut microbiome is associated with colorectal cancer. The results obtained support the possibility of using molecular genetic detection of the studied potentially oncogenic microorganisms as a method for non-invasive diagnosis of CRC. |
| format | Article |
| id | doaj-art-98fa9d6abdc54b1c9534a3a1fa06710f |
| institution | Kabale University |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| publishDate | 2024-11-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj-art-98fa9d6abdc54b1c9534a3a1fa06710f2025-01-09T20:09:08ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132024-11-01101566867810.36233/0372-9311-5642788Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancerViktoriya N. Shumilova0https://orcid.org/0009-0004-4271-6530Artemij E. Goncharov1https://orcid.org/0000-0002-5206-6656Daniil V. Azarov2https://orcid.org/0000-0003-2483-5144Stanislav I. Sitkin3Elgudzha L. Latariya4https://orcid.org/0000-0002-9569-8485Batyrbek I. Aslanov5https://orcid.org/0000-0002-6890-8096Mikhail A. Bobrakov6https://orcid.org/0009-0003-3003-8886Rustem E. Topuzov7https://orcid.org/0000-0001-8168-6187North-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovRelevance. Colorectal cancer (CRC) is the second leading cause of cancer mortality worldwide. Non-invasive diagnostic methods based on the determination of hidden blood in the stool (fecal immunochemical test, guaiac test), which have been proven to be effective in clinical studies, are used for CRC screening. However, a significant disadvantage of the available non-invasive diagnostic methods is the low sensitivity in detecting the oncological process at the early stages. A number of recent studies discuss the relationship between the disease and various potentially oncogenic microorganisms in the human intestinal tract, which can be used to expand the arsenal of non-invasive methods for diagnosing CRC based on molecular genetic examination of a stool sample to identify oncogenic microorganisms. The aim of this study was to evaluate the possibility of using genetic determinants of potentially oncogenic microorganisms as markers for colorectal cancer, based on a comparison of their prevalence in groups of patients with colorectal cancer, facultative precancerous diseases and patients without intestinal pathology. Materials and methods. 215 participants were included in the "case–control" study: 70 patients with newly diagnosed colorectal cancer, 70 patients with inflammatory bowel disease, 75 participants without diagnosed intestinal pathology. Polymerase chain reaction (PCR) was used to identify and detect genes of potentially oncogenic microorganisms. Results and discussion. An association was found between CRC and the presence of the Bacteroides fragilis fragilisin gene (OR 7.00; 95% CI: 2.55–22.50; p 0.001), species-specific genes of the periodontal pathogenic microorganisms Fusobacterium nucleatum (OR 5.61; 95% CI: 2.87–11.30; p 0.001) and Porphyromonas gingivalis (OR 16.3; 95% CI: 4.33–106.00; p 0.001), the clbB gene of pks pathogenicity island of the Enterobacteria (OR 3.44; 95% CI: 1.39–8.51; p = 0.010). Conclusion. The presence of genetic markers of potentially oncogenic bacterial species and genotypes in the gut microbiome is associated with colorectal cancer. The results obtained support the possibility of using molecular genetic detection of the studied potentially oncogenic microorganisms as a method for non-invasive diagnosis of CRC.https://microbiol.crie.ru/jour/article/viewFile/18688/1523colorectal cancermicrobiotamicrobial markerspotentially oncogenic microorganismsnon-invasive diagnosisscreening |
| spellingShingle | Viktoriya N. Shumilova Artemij E. Goncharov Daniil V. Azarov Stanislav I. Sitkin Elgudzha L. Latariya Batyrbek I. Aslanov Mikhail A. Bobrakov Rustem E. Topuzov Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer Журнал микробиологии, эпидемиологии и иммунобиологии colorectal cancer microbiota microbial markers potentially oncogenic microorganisms non-invasive diagnosis screening |
| title | Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer |
| title_full | Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer |
| title_fullStr | Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer |
| title_full_unstemmed | Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer |
| title_short | Detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer |
| title_sort | detection of genetic determinants of potentially oncogenic representatives of the intestinal microbiota as biomarkers of colorectal cancer |
| topic | colorectal cancer microbiota microbial markers potentially oncogenic microorganisms non-invasive diagnosis screening |
| url | https://microbiol.crie.ru/jour/article/viewFile/18688/1523 |
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