Phytochemical Profile and Biological Activity of the Ethanol Extracts from the Aerial Parts of <i>Adonis tianschanica</i> (Adolf.) Lipsch. Growing in Kazakhstan

<i>Adonis tianschanica</i> is a lesser-known plant species belonging to the genus <i>Adonis</i> that grows in Kazakhstan. The aim of this study was to characterize the composition of the ethanolic, water, and hydroethanolic extracts from the aerial parts of <i>A. tiansc...

Full description

Saved in:
Bibliographic Details
Main Authors: Saule Orynbekova, Wirginia Kukula-Koch, Zuriyadda Sakipova, Bashaer Alsharif, Beibhinn Rafferty, Talgat Nurgozhin, Zoya Allambergenova, Piotr Dreher, Kazimierz Głowniak, Fabio Boylan
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/29/23/5754
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Adonis tianschanica</i> is a lesser-known plant species belonging to the genus <i>Adonis</i> that grows in Kazakhstan. The aim of this study was to characterize the composition of the ethanolic, water, and hydroethanolic extracts from the aerial parts of <i>A. tianschanica</i> by HPLC-ESI-QTOF-MS/MS to isolate the major compound isoquercitrin by HSCCC (High-Speed Counter-Current Chromatography) and to determine the cytotoxicity and anti-inflammatory potential of the extracts produced with this plant. Fingerprinting of the analyzed extracts showed the presence of a multitude of metabolites comprising polyphenols, organic acids, and coumarins, and only trace quantities of cardiac glycosides in the analyzed samples. Flavonoids were certainly the best-represented group, with kaempferol, quercetin, and their derivatives as the major components of the extracts. Key findings in this paper were that the ethanol: water (50:50 <i>v</i>/<i>v</i>) extract of <i>A. tianschanica</i> and its major compound isoquercitrin were able to reduce the production of NO induced by LPS, in addition to demonstrating anti-inflammatory effects by reducing cytokines such as IL-6, TNF-α, and IL-1β.
ISSN:1420-3049