Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis
Abstract Orthosiphon aristatus (O. aristatus) has been used as a popular traditional folk medicine for the treatment of kidney disease. Recent studies have shown that O. aristatus root contains more flavonoids and has higher antioxidant capacity compared to its medicinal parts. However, there is lim...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-85266-x |
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| author | Qiaoxue Wang Huan Long Shumeng Liu Xixi Xu Qin Zhang Shanyong Yi |
| author_facet | Qiaoxue Wang Huan Long Shumeng Liu Xixi Xu Qin Zhang Shanyong Yi |
| author_sort | Qiaoxue Wang |
| collection | DOAJ |
| description | Abstract Orthosiphon aristatus (O. aristatus) has been used as a popular traditional folk medicine for the treatment of kidney disease. Recent studies have shown that O. aristatus root contains more flavonoids and has higher antioxidant capacity compared to its medicinal parts. However, there is limited knowledge on the mechanisms behind the synthetic biology of flavonoid in all tissues of O. aristatus. Here, we performed high-performance liquid chromatography (HPLC) analysis and comparative transcriptome analyses of flavonoids in all tissues. The content of 4 major flavonoids is significantly higher in roots and leaves compared to stems in O. aristatus. Out of a total of 138,294 Unigenes, the KEGG pathway analysis identified 66 Unigenes encoding 8 key metabolic enzymes involved in the biosynthetic pathway of flavonoid compounds. Additionally, 5,154 on average DEGs involved in flavonoid biosynthesis could be categorised into 58 transcription factor (TF) families. Among the identified DEGs, a total of 5,897 were common to all tissues, with 212 DEGs strongly associated with flavonoid accumulation in root. Several of these key enzyme genes were further validated by quantitative real-time polymerase chain reaction (qRT-PCR). Our research provides novel insights into flavonoids synthetic biology, and highlights O. aristatus root may serve as a valuable resource for medicinal use. |
| format | Article |
| id | doaj-art-97d28298eea643caa4be7ed040d0857b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-97d28298eea643caa4be7ed040d0857b2025-01-12T12:20:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-85266-xComparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesisQiaoxue Wang0Huan Long1Shumeng Liu2Xixi Xu3Qin Zhang4Shanyong Yi5School of Medicine, Department of Pharmacy, Zhongda Hospital, Southeast UniversitySchool of Medicine, Department of Pharmacy, Zhongda Hospital, Southeast UniversityDepartment of Pharmacy, Affiliated Hospital of Nanjing University of Chinese MedicineSchool of Medicine, Department of Pharmacy, Zhongda Hospital, Southeast UniversityDepartment of Pharmacy, Affiliated Hospital of Nanjing University of Chinese MedicineAnhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine, West Anhui UniversityAbstract Orthosiphon aristatus (O. aristatus) has been used as a popular traditional folk medicine for the treatment of kidney disease. Recent studies have shown that O. aristatus root contains more flavonoids and has higher antioxidant capacity compared to its medicinal parts. However, there is limited knowledge on the mechanisms behind the synthetic biology of flavonoid in all tissues of O. aristatus. Here, we performed high-performance liquid chromatography (HPLC) analysis and comparative transcriptome analyses of flavonoids in all tissues. The content of 4 major flavonoids is significantly higher in roots and leaves compared to stems in O. aristatus. Out of a total of 138,294 Unigenes, the KEGG pathway analysis identified 66 Unigenes encoding 8 key metabolic enzymes involved in the biosynthetic pathway of flavonoid compounds. Additionally, 5,154 on average DEGs involved in flavonoid biosynthesis could be categorised into 58 transcription factor (TF) families. Among the identified DEGs, a total of 5,897 were common to all tissues, with 212 DEGs strongly associated with flavonoid accumulation in root. Several of these key enzyme genes were further validated by quantitative real-time polymerase chain reaction (qRT-PCR). Our research provides novel insights into flavonoids synthetic biology, and highlights O. aristatus root may serve as a valuable resource for medicinal use.https://doi.org/10.1038/s41598-025-85266-x |
| spellingShingle | Qiaoxue Wang Huan Long Shumeng Liu Xixi Xu Qin Zhang Shanyong Yi Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis Scientific Reports |
| title | Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis |
| title_full | Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis |
| title_fullStr | Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis |
| title_full_unstemmed | Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis |
| title_short | Comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis |
| title_sort | comparative transcriptome analyses of different orthosiphon aristatus tissues reveal differentially expressed genes associated with flavonoid biosynthesis |
| url | https://doi.org/10.1038/s41598-025-85266-x |
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