Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes
Abstract Entomopathogenic nematodes (EPNs) associated with their symbiotic bacteria can effectively kill insect pests, in agriculture, forestry and floriculture. Industrial-scale production techniques for EPNs have been established, including solid and liquid monoculture systems. It is found that su...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-12-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-80913-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559508026916864 |
|---|---|
| author | Xingxin Liu Zhongchen Rao Richou Han Linhong Jin Li Cao |
| author_facet | Xingxin Liu Zhongchen Rao Richou Han Linhong Jin Li Cao |
| author_sort | Xingxin Liu |
| collection | DOAJ |
| description | Abstract Entomopathogenic nematodes (EPNs) associated with their symbiotic bacteria can effectively kill insect pests, in agriculture, forestry and floriculture. Industrial-scale production techniques for EPNs have been established, including solid and liquid monoculture systems. It is found that supplement of 0.01% dimethyl sulfoxide (DMSO) to the culture medium significantly enhances the recovery and infective juvenile yield of these nematodes. However, the specific nematode genes responding to DMSO is not yet clear. This study identified differentially expressed genes and associated enriched pathways by analyzing the transcriptomic response of Heterorhabditis bacteriophora H06 in the liquid medium containing 0.01% DMSO, and validated the function of the selected genes using RNA interference (RNAi). 11 upregulated and 295 downregulated genes were detected in the hermaphrodites of H. bacteriophora H06 at 3 days beforee egg formation, following DMSO treatment. The biological processes involved included regulation of biological processes, metabolism, binding, signal transduction, post-transcriptional modifications, metabolism, and protein folding. By using RNAi, knockdown of three genes, Hint module, PAN domain protein, and Dpy-13, significantly regulated the egg load (at least a 17% increase in eggs per hermaphroditic adult) and the yield of infective juveniles (at least a 48% increase), in an optimized 96-well plates containing the medium with 1.6% nutrient broth, 1% corn oil and 1.2% agar, without DMSO. qRT-PCR results confirmed that the expression levels of the knockdown genes decreased significantly, compared to the control. These results demonstrate that DMSO regulates nematode gravidity by suppressing at least three selected genes, thereby enhancing IJ yield, providing molecular cues for understanding how DMSO regulates the EPN yield, and a new technology for enhancing nematode commercial production. |
| format | Article |
| id | doaj-art-97c20b83e9ea42b88beba6ff4d148615 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-97c20b83e9ea42b88beba6ff4d1486152025-01-05T12:27:01ZengNature PortfolioScientific Reports2045-23222024-12-0114111110.1038/s41598-024-80913-1Genes involved in DMSO-mediated yield increase of entomopathogenic nematodesXingxin Liu0Zhongchen Rao1Richou Han2Linhong Jin3Li Cao4State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education Center for R&D of Fine Chemicals of Guizhou UniversityGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of SciencesGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of SciencesState Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education Center for R&D of Fine Chemicals of Guizhou UniversityGuangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of SciencesAbstract Entomopathogenic nematodes (EPNs) associated with their symbiotic bacteria can effectively kill insect pests, in agriculture, forestry and floriculture. Industrial-scale production techniques for EPNs have been established, including solid and liquid monoculture systems. It is found that supplement of 0.01% dimethyl sulfoxide (DMSO) to the culture medium significantly enhances the recovery and infective juvenile yield of these nematodes. However, the specific nematode genes responding to DMSO is not yet clear. This study identified differentially expressed genes and associated enriched pathways by analyzing the transcriptomic response of Heterorhabditis bacteriophora H06 in the liquid medium containing 0.01% DMSO, and validated the function of the selected genes using RNA interference (RNAi). 11 upregulated and 295 downregulated genes were detected in the hermaphrodites of H. bacteriophora H06 at 3 days beforee egg formation, following DMSO treatment. The biological processes involved included regulation of biological processes, metabolism, binding, signal transduction, post-transcriptional modifications, metabolism, and protein folding. By using RNAi, knockdown of three genes, Hint module, PAN domain protein, and Dpy-13, significantly regulated the egg load (at least a 17% increase in eggs per hermaphroditic adult) and the yield of infective juveniles (at least a 48% increase), in an optimized 96-well plates containing the medium with 1.6% nutrient broth, 1% corn oil and 1.2% agar, without DMSO. qRT-PCR results confirmed that the expression levels of the knockdown genes decreased significantly, compared to the control. These results demonstrate that DMSO regulates nematode gravidity by suppressing at least three selected genes, thereby enhancing IJ yield, providing molecular cues for understanding how DMSO regulates the EPN yield, and a new technology for enhancing nematode commercial production.https://doi.org/10.1038/s41598-024-80913-1Entomopathogenic nematodeDMSOTranscriptomeRNAi |
| spellingShingle | Xingxin Liu Zhongchen Rao Richou Han Linhong Jin Li Cao Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes Scientific Reports Entomopathogenic nematode DMSO Transcriptome RNAi |
| title | Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes |
| title_full | Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes |
| title_fullStr | Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes |
| title_full_unstemmed | Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes |
| title_short | Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes |
| title_sort | genes involved in dmso mediated yield increase of entomopathogenic nematodes |
| topic | Entomopathogenic nematode DMSO Transcriptome RNAi |
| url | https://doi.org/10.1038/s41598-024-80913-1 |
| work_keys_str_mv | AT xingxinliu genesinvolvedindmsomediatedyieldincreaseofentomopathogenicnematodes AT zhongchenrao genesinvolvedindmsomediatedyieldincreaseofentomopathogenicnematodes AT richouhan genesinvolvedindmsomediatedyieldincreaseofentomopathogenicnematodes AT linhongjin genesinvolvedindmsomediatedyieldincreaseofentomopathogenicnematodes AT licao genesinvolvedindmsomediatedyieldincreaseofentomopathogenicnematodes |