Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng
Endophytes, symbiotically with their host plants, are frequently found in the roots of Panax ginseng. To explore ginsenosides biotransformation by endophytic fungi, 26 such fungi were isolated from ginseng roots sourced from four distinct planting regions, and their glycosyl hydrolysis activities we...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2024.2362852 |
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| author | Linggai Cao Shizhou Yu Lanlan Zhang Xiaolian Zhang Yuhua Li |
| author_facet | Linggai Cao Shizhou Yu Lanlan Zhang Xiaolian Zhang Yuhua Li |
| author_sort | Linggai Cao |
| collection | DOAJ |
| description | Endophytes, symbiotically with their host plants, are frequently found in the roots of Panax ginseng. To explore ginsenosides biotransformation by endophytic fungi, 26 such fungi were isolated from ginseng roots sourced from four distinct planting regions, and their glycosyl hydrolysis activities were measured. Of these endophytic, 20 isolates exhibited glycosidase enzymes capable of cleaving glycosidic bonds of glucose, arabinofuranose and arabinopyranose. Notably, the glycosidase BglNh gene was isolated and cloned from Nectria haematococca, one of the endophytic fungi. Enzymatic assessments unveiled that BglNh specifically catalyzes the hydrolysis of glucose residues located external to the C-3 position of protopanaxadiol ginsenosides under optimal reaction conditions (pH 6.0 and temperature 35 °C). Our findings demonstrate the efficient conversion of major ginsenosides Rb1, Rb2 and Rc to minor ginsenosides Gyp17, CO and CMc1, highlighting the potential of these fungi for ginsenoside biotransformation and bioactive compound production. |
| format | Article |
| id | doaj-art-979b64e988044e3fbc15af651f516c7c |
| institution | Kabale University |
| issn | 1310-2818 1314-3530 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj-art-979b64e988044e3fbc15af651f516c7c2024-12-10T05:42:51ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2362852Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginsengLinggai Cao0Shizhou Yu1Lanlan Zhang2Xiaolian Zhang3Yuhua Li4Laboratory of Biological Effects and Biosynthesis, Department of Research 2, Beijing Life Science Academy (BLSA), Beijing, PR ChinaKey Laboratory of Molecular Genetics, Department of Breeding Engineering Center, Guizhou Academy of Tobacco Science, Guiyang, Guizhou, PR ChinaDepartment of Biotechnology, College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, PR ChinaKey Laboratory of Molecular Genetics, Department of Breeding Engineering Center, Guizhou Academy of Tobacco Science, Guiyang, Guizhou, PR ChinaKey Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Science, Northeast Forestry University, Harbin, Heilongjiang, PR ChinaEndophytes, symbiotically with their host plants, are frequently found in the roots of Panax ginseng. To explore ginsenosides biotransformation by endophytic fungi, 26 such fungi were isolated from ginseng roots sourced from four distinct planting regions, and their glycosyl hydrolysis activities were measured. Of these endophytic, 20 isolates exhibited glycosidase enzymes capable of cleaving glycosidic bonds of glucose, arabinofuranose and arabinopyranose. Notably, the glycosidase BglNh gene was isolated and cloned from Nectria haematococca, one of the endophytic fungi. Enzymatic assessments unveiled that BglNh specifically catalyzes the hydrolysis of glucose residues located external to the C-3 position of protopanaxadiol ginsenosides under optimal reaction conditions (pH 6.0 and temperature 35 °C). Our findings demonstrate the efficient conversion of major ginsenosides Rb1, Rb2 and Rc to minor ginsenosides Gyp17, CO and CMc1, highlighting the potential of these fungi for ginsenoside biotransformation and bioactive compound production.https://www.tandfonline.com/doi/10.1080/13102818.2024.2362852Ginsenosideglycoside hydrolasesendophytic fungus of Panax ginsengbiotransform |
| spellingShingle | Linggai Cao Shizhou Yu Lanlan Zhang Xiaolian Zhang Yuhua Li Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng Biotechnology & Biotechnological Equipment Ginsenoside glycoside hydrolases endophytic fungus of Panax ginseng biotransform |
| title | Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng |
| title_full | Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng |
| title_fullStr | Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng |
| title_full_unstemmed | Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng |
| title_short | Biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of Panax ginseng |
| title_sort | biotransformation of ginsenosides by glycoside hydrolase from an endophytic fungus of panax ginseng |
| topic | Ginsenoside glycoside hydrolases endophytic fungus of Panax ginseng biotransform |
| url | https://www.tandfonline.com/doi/10.1080/13102818.2024.2362852 |
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