Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives
Abstract Bacterial biofilm infection is a complicated and important global problem. Non-toxic biomass-based Pickering emulsions as an essential oil delivery system are a potentially effective means in biofilm treatment. Herein, the chitosan/gelatin complex nanoparticles (CGNPs) stabilized Pickering...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-01-01
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| Series: | Collagen and Leather |
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| Online Access: | https://doi.org/10.1186/s42825-024-00180-8 |
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| author | Zhilang Xu Xinxin Wang Huaqian Liang Xinying Li Derong Li Changdao Mu Liming Ge Defu Li |
| author_facet | Zhilang Xu Xinxin Wang Huaqian Liang Xinying Li Derong Li Changdao Mu Liming Ge Defu Li |
| author_sort | Zhilang Xu |
| collection | DOAJ |
| description | Abstract Bacterial biofilm infection is a complicated and important global problem. Non-toxic biomass-based Pickering emulsions as an essential oil delivery system are a potentially effective means in biofilm treatment. Herein, the chitosan/gelatin complex nanoparticles (CGNPs) stabilized Pickering (CGP) emulsion loaded with cinnamon essential oil (CEO) was developed to inhibit and remove bacterial biofilms. CGNPs were initially successfully prepared with a particle size of 253.7 nm. Subsequently, CGP emulsions were prepared using CGNPs as the emulsifier and CEO as the oil phase, with the aqueous phase pH value of 5.5 and ionic strength of 50 mM. Amino groups of CGNPs were crosslinked with the aldehyde group of CEO via Schiff base reaction at the oil–water interface to form a denser core–shell structure, thus improving the stability of Pickering emulsion. The CGP emulsions exhibit controlled and long-term sustained release properties in essential oil delivery, with 89.8%, 81.0%, and 62.2% CEO release within 168 h for CGP-0.1, CGP-0.2, and CGP-0.3, respectively. Moreover, CGP emulsions have stronger antioxidant activities (nearly 100%), broad-spectrum antibacterial activities (> 90%), enhanced penetration and removal capabilities against preformed biofilms than single CEO or CGNPs, as well as its better biocompatibility than conventional surfactant-stabilized emulsions. Hence, CEO-delivered CGP emulsion could serve as a potential strategy against biofilm infections and bacterial resistance. Graphical abstract |
| format | Article |
| id | doaj-art-97839d2a268b44b9bc55f1449ac81564 |
| institution | Kabale University |
| issn | 2097-1419 2731-6998 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
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| series | Collagen and Leather |
| spelling | doaj-art-97839d2a268b44b9bc55f1449ac815642025-01-05T12:46:03ZengSpringerOpenCollagen and Leather2097-14192731-69982025-01-017111810.1186/s42825-024-00180-8Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm activesZhilang Xu0Xinxin Wang1Huaqian Liang2Xinying Li3Derong Li4Changdao Mu5Liming Ge6Defu Li7Department of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan UniversityDepartment of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan UniversityDepartment of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan UniversityCollege of Chemistry and Environment Protection Engineering, Southwest Minzu UniversityPeople’s Hospital of Lanshan DistrictDepartment of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan UniversityDepartment of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan UniversityDepartment of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan UniversityAbstract Bacterial biofilm infection is a complicated and important global problem. Non-toxic biomass-based Pickering emulsions as an essential oil delivery system are a potentially effective means in biofilm treatment. Herein, the chitosan/gelatin complex nanoparticles (CGNPs) stabilized Pickering (CGP) emulsion loaded with cinnamon essential oil (CEO) was developed to inhibit and remove bacterial biofilms. CGNPs were initially successfully prepared with a particle size of 253.7 nm. Subsequently, CGP emulsions were prepared using CGNPs as the emulsifier and CEO as the oil phase, with the aqueous phase pH value of 5.5 and ionic strength of 50 mM. Amino groups of CGNPs were crosslinked with the aldehyde group of CEO via Schiff base reaction at the oil–water interface to form a denser core–shell structure, thus improving the stability of Pickering emulsion. The CGP emulsions exhibit controlled and long-term sustained release properties in essential oil delivery, with 89.8%, 81.0%, and 62.2% CEO release within 168 h for CGP-0.1, CGP-0.2, and CGP-0.3, respectively. Moreover, CGP emulsions have stronger antioxidant activities (nearly 100%), broad-spectrum antibacterial activities (> 90%), enhanced penetration and removal capabilities against preformed biofilms than single CEO or CGNPs, as well as its better biocompatibility than conventional surfactant-stabilized emulsions. Hence, CEO-delivered CGP emulsion could serve as a potential strategy against biofilm infections and bacterial resistance. Graphical abstracthttps://doi.org/10.1186/s42825-024-00180-8Pickering emulsionCinnamon essential oilMulticomponent nanoparticlesBacterial resistanceAntibiofilm |
| spellingShingle | Zhilang Xu Xinxin Wang Huaqian Liang Xinying Li Derong Li Changdao Mu Liming Ge Defu Li Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives Collagen and Leather Pickering emulsion Cinnamon essential oil Multicomponent nanoparticles Bacterial resistance Antibiofilm |
| title | Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives |
| title_full | Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives |
| title_fullStr | Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives |
| title_full_unstemmed | Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives |
| title_short | Development of cinnamon essential oil-loaded Pickering emulsions stabilized by chitosan/gelatin nanoparticles with enhanced antibacterial and antibiofilm actives |
| title_sort | development of cinnamon essential oil loaded pickering emulsions stabilized by chitosan gelatin nanoparticles with enhanced antibacterial and antibiofilm actives |
| topic | Pickering emulsion Cinnamon essential oil Multicomponent nanoparticles Bacterial resistance Antibiofilm |
| url | https://doi.org/10.1186/s42825-024-00180-8 |
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