Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery

The application of electrospinning technologies for the preparation of mats based on mucoadhesive polysaccharides, such as chitosan (CS), is an attractive strategy for the development of biopolymeric delivery systems for topical corticosteroids. In this work, an electrospinning technique is describe...

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Main Authors: Natallia V. Dubashynskaya, Valentina A. Petrova, Igor V. Kudryavtsev, Andrey S. Trulioff, Artem A. Rubinstein, Alexey S. Golovkin, Alexander I. Mishanin, Anton A. Murav’ev, Iosif V. Gofman, Daria N. Poshina, Yury A. Skorik
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Language:English
Published: MDPI AG 2025-02-01
Series:Technologies
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Online Access:https://www.mdpi.com/2227-7080/13/3/90
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author Natallia V. Dubashynskaya
Valentina A. Petrova
Igor V. Kudryavtsev
Andrey S. Trulioff
Artem A. Rubinstein
Alexey S. Golovkin
Alexander I. Mishanin
Anton A. Murav’ev
Iosif V. Gofman
Daria N. Poshina
Yury A. Skorik
author_facet Natallia V. Dubashynskaya
Valentina A. Petrova
Igor V. Kudryavtsev
Andrey S. Trulioff
Artem A. Rubinstein
Alexey S. Golovkin
Alexander I. Mishanin
Anton A. Murav’ev
Iosif V. Gofman
Daria N. Poshina
Yury A. Skorik
author_sort Natallia V. Dubashynskaya
collection DOAJ
description The application of electrospinning technologies for the preparation of mats based on mucoadhesive polysaccharides, such as chitosan (CS), is an attractive strategy for the development of biopolymeric delivery systems for topical corticosteroids. In this work, an electrospinning technique is described for the preparation of CS-based mats doped with halloysite nanotubes (HNTs) with modified release of clobetasol propionate (CP). The optimized composition of the electrospinning solution was determined: 2.4% solution of CS in 46% acetic acid with addition of PEO (10% of CS mass) and HNTs (5% of CS mass); CP was introduced as an ethanol solution at the rate of 2 mg CP per 1 g of the obtained nonwoven material. The process parameters (the electrospinning voltage of 50–65 kV, the rotation speed of the spinning electrode of 10 min<sup>−1</sup>, and the distance between the electrodes of 24 cm) were also optimized. The developed technology allowed us to obtain homogeneous nanofiber mats with excellent mechanical properties and biphasic drug release patterns (66% of CP released within 0.5 h and 88% of CP released within 6 h). The obtained nanofiber mats maintained the anti-inflammatory activity of corticosteroid at the level of free CP and showed no cytotoxicity.
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spelling doaj-art-97653204d33d46f3ad26a00f11aed6b22025-08-20T03:44:03ZengMDPI AGTechnologies2227-70802025-02-011339010.3390/technologies13030090Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate DeliveryNatallia V. Dubashynskaya0Valentina A. Petrova1Igor V. Kudryavtsev2Andrey S. Trulioff3Artem A. Rubinstein4Alexey S. Golovkin5Alexander I. Mishanin6Anton A. Murav’ev7Iosif V. Gofman8Daria N. Poshina9Yury A. Skorik10Branch of Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»-Institute of Macromolecular Compounds, Bolshoi VO 31, St. Petersburg 199004, RussiaBranch of Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»-Institute of Macromolecular Compounds, Bolshoi VO 31, St. Petersburg 199004, RussiaInstitute of Experimental Medicine, Acad. Pavlova 12, St. Petersburg 197022, RussiaInstitute of Experimental Medicine, Acad. Pavlova 12, St. Petersburg 197022, RussiaInstitute of Experimental Medicine, Acad. Pavlova 12, St. Petersburg 197022, RussiaAlmazov National Medical Research Centre, Akkuratova 2, St. Petersburg 197341, RussiaAlmazov National Medical Research Centre, Akkuratova 2, St. Petersburg 197341, RussiaBranch of Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»-Institute of Macromolecular Compounds, Bolshoi VO 31, St. Petersburg 199004, RussiaBranch of Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»-Institute of Macromolecular Compounds, Bolshoi VO 31, St. Petersburg 199004, RussiaBranch of Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»-Institute of Macromolecular Compounds, Bolshoi VO 31, St. Petersburg 199004, RussiaBranch of Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»-Institute of Macromolecular Compounds, Bolshoi VO 31, St. Petersburg 199004, RussiaThe application of electrospinning technologies for the preparation of mats based on mucoadhesive polysaccharides, such as chitosan (CS), is an attractive strategy for the development of biopolymeric delivery systems for topical corticosteroids. In this work, an electrospinning technique is described for the preparation of CS-based mats doped with halloysite nanotubes (HNTs) with modified release of clobetasol propionate (CP). The optimized composition of the electrospinning solution was determined: 2.4% solution of CS in 46% acetic acid with addition of PEO (10% of CS mass) and HNTs (5% of CS mass); CP was introduced as an ethanol solution at the rate of 2 mg CP per 1 g of the obtained nonwoven material. The process parameters (the electrospinning voltage of 50–65 kV, the rotation speed of the spinning electrode of 10 min<sup>−1</sup>, and the distance between the electrodes of 24 cm) were also optimized. The developed technology allowed us to obtain homogeneous nanofiber mats with excellent mechanical properties and biphasic drug release patterns (66% of CP released within 0.5 h and 88% of CP released within 6 h). The obtained nanofiber mats maintained the anti-inflammatory activity of corticosteroid at the level of free CP and showed no cytotoxicity.https://www.mdpi.com/2227-7080/13/3/90chitosanhalloysite nanotubesclobetasol propionateelectrospun matsoromucosal drug delivery systemsanti-inflammatory activity
spellingShingle Natallia V. Dubashynskaya
Valentina A. Petrova
Igor V. Kudryavtsev
Andrey S. Trulioff
Artem A. Rubinstein
Alexey S. Golovkin
Alexander I. Mishanin
Anton A. Murav’ev
Iosif V. Gofman
Daria N. Poshina
Yury A. Skorik
Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery
Technologies
chitosan
halloysite nanotubes
clobetasol propionate
electrospun mats
oromucosal drug delivery systems
anti-inflammatory activity
title Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery
title_full Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery
title_fullStr Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery
title_full_unstemmed Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery
title_short Electrospinning of Chitosan–Halloysite Nanotube Biohybrid Mats for Clobetasol Propionate Delivery
title_sort electrospinning of chitosan halloysite nanotube biohybrid mats for clobetasol propionate delivery
topic chitosan
halloysite nanotubes
clobetasol propionate
electrospun mats
oromucosal drug delivery systems
anti-inflammatory activity
url https://www.mdpi.com/2227-7080/13/3/90
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