T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响

【目的】观察气道内T-bet基因转染对哮喘小鼠脾脏淋巴细胞Th1/Th2平衡65380;T-bet及GATA-3 mRNA表达的影响65377; 【方法】将C57BL/6小鼠32只随机分为4组,每组8只,分别为正常对照组(A组)65380;哮喘模型组(B组)65380;空质粒干预组(C组) 和T-bet质粒干预组(D组)65377;卵白蛋白(OVA) 抗原溶液腹腔注射致敏,滴鼻造模65377;空质粒组和T-bet质粒干预组OVA激发24 h前,分别经鼻滴入50 μg空质粒65380;重组T-bet质粒,对照组用生理盐水代替OVA65377;最后一次滴鼻激发48 h后处死小鼠,流式细胞仪检测...

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Format:	Article
Language:	zho
Published:	Editorial Office of Journal of Sun Yat-sen University 2010-01-01
Series:	Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:	哮喘 T-bet GATA-3 Th1 Th2
Online Access:	http://xuebaoyx.sysu.edu.cn/zh/article/43577158/
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_version_	1841530389531721728
collection	DOAJ
description	【目的】观察气道内T-bet基因转染对哮喘小鼠脾脏淋巴细胞Th1/Th2平衡65380;T-bet及GATA-3 mRNA表达的影响65377; 【方法】将C57BL/6小鼠32只随机分为4组,每组8只,分别为正常对照组(A组)65380;哮喘模型组(B组)65380;空质粒干预组(C组) 和T-bet质粒干预组(D组)65377;卵白蛋白(OVA) 抗原溶液腹腔注射致敏,滴鼻造模65377;空质粒组和T-bet质粒干预组OVA激发24 h前,分别经鼻滴入50 μg空质粒65380;重组T-bet质粒,对照组用生理盐水代替OVA65377;最后一次滴鼻激发48 h后处死小鼠,流式细胞仪检测各组实验小鼠的脾脏淋巴细胞的Th1/Th2, RT-PCR检测小鼠脾脏淋巴细胞的T-bet及GATA-3mRNA表达65377; 【结果】哮喘小鼠气道转染pcDNA3-T-bet质粒后:①流式细胞仪检测发现,脾脏Th1百分率较哮喘模型组显著升高[(2.29 ± 1.55)% vs. (1.93 ± 1.14)%,P﹤0.05],Th2百分率显著降低[(0.93 ± 0.64)% vs. (1.63 ± 0.59)%];②RT-PCR检测发现脾脏淋巴细胞转录因子T-bet mRNA表达水平显著增加(0.53 ± 0.027 vs. 0.28 ± 0.035,P﹤0.05), GATA-3 mRNA表达水平显著降低(0.24 ± 0.022 vs. 0.58 ± 0.038,P﹤0.05)65377;而pcDNA3空质粒干预后与哮喘模型组比较无显著差异65377;【结论】气道内转导pcDNA3-T-bet质粒后,可显著上调哮喘小鼠体内T-betmRNA表达,下调GATA-3 mRNA表达,有效改善哮喘小鼠体内Th1/Th2比例失衡,从而抑制哮喘小鼠的气道炎症,为哮喘的T-bet基因治疗提供依据65377;
format	Article
id	doaj-art-975b6c4a5a3f463088ade95794629143
institution	Kabale University
issn	1672-3554
language	zho
publishDate	2010-01-01
publisher	Editorial Office of Journal of Sun Yat-sen University
record_format	Article
series	Zhongshan Daxue xuebao. Yixue kexue ban
spelling	doaj-art-975b6c4a5a3f463088ade957946291432025-01-15T03:04:37ZzhoEditorial Office of Journal of Sun Yat-sen UniversityZhongshan Daxue xuebao. Yixue kexue ban1672-35542010-01-013143577158T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响【目的】观察气道内T-bet基因转染对哮喘小鼠脾脏淋巴细胞Th1/Th2平衡65380;T-bet及GATA-3 mRNA表达的影响65377; 【方法】将C57BL/6小鼠32只随机分为4组,每组8只,分别为正常对照组(A组)65380;哮喘模型组(B组)65380;空质粒干预组(C组) 和T-bet质粒干预组(D组)65377;卵白蛋白(OVA) 抗原溶液腹腔注射致敏,滴鼻造模65377;空质粒组和T-bet质粒干预组OVA激发24 h前,分别经鼻滴入50 μg空质粒65380;重组T-bet质粒,对照组用生理盐水代替OVA65377;最后一次滴鼻激发48 h后处死小鼠,流式细胞仪检测各组实验小鼠的脾脏淋巴细胞的Th1/Th2, RT-PCR检测小鼠脾脏淋巴细胞的T-bet及GATA-3mRNA表达65377; 【结果】哮喘小鼠气道转染pcDNA3-T-bet质粒后:①流式细胞仪检测发现,脾脏Th1百分率较哮喘模型组显著升高[(2.29 ± 1.55)% vs. (1.93 ± 1.14)%,P﹤0.05],Th2百分率显著降低[(0.93 ± 0.64)% vs. (1.63 ± 0.59)%];②RT-PCR检测发现脾脏淋巴细胞转录因子T-bet mRNA表达水平显著增加(0.53 ± 0.027 vs. 0.28 ± 0.035,P﹤0.05), GATA-3 mRNA表达水平显著降低(0.24 ± 0.022 vs. 0.58 ± 0.038,P﹤0.05)65377;而pcDNA3空质粒干预后与哮喘模型组比较无显著差异65377;【结论】气道内转导pcDNA3-T-bet质粒后,可显著上调哮喘小鼠体内T-betmRNA表达,下调GATA-3 mRNA表达,有效改善哮喘小鼠体内Th1/Th2比例失衡,从而抑制哮喘小鼠的气道炎症,为哮喘的T-bet基因治疗提供依据65377;http://xuebaoyx.sysu.edu.cn/zh/article/43577158/哮喘T-betGATA-3Th1Th2
spellingShingle	T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响 Zhongshan Daxue xuebao. Yixue kexue ban 哮喘 T-bet GATA-3 Th1 Th2
title	T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响
title_full	T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响
title_fullStr	T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响
title_full_unstemmed	T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响
title_short	T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响
title_sort	t bet基因转染对哮喘小鼠脾脏th1 th2平衡的影响
topic	哮喘 T-bet GATA-3 Th1 Th2
url	http://xuebaoyx.sysu.edu.cn/zh/article/43577158/

T-bet基因转染对哮喘小鼠脾脏Th1/Th2平衡的影响

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