Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients

Abstract The tumor microenvironment (TME), particularly CD8+ T cell infiltration, critically influences high-grade serous ovarian cancer (HGSOC) progression and treatment response. The development and management of cancer depend heavily on CD8+ T cells. Identifying non-invasive predictors of TME imm...

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Main Authors: Jia Mai, Ling Yang, YuXin Chen, XiaoXu Zeng, HongJian Xie, XiaoJuan Liu
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-14720-7
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author Jia Mai
Ling Yang
YuXin Chen
XiaoXu Zeng
HongJian Xie
XiaoJuan Liu
author_facet Jia Mai
Ling Yang
YuXin Chen
XiaoXu Zeng
HongJian Xie
XiaoJuan Liu
author_sort Jia Mai
collection DOAJ
description Abstract The tumor microenvironment (TME), particularly CD8+ T cell infiltration, critically influences high-grade serous ovarian cancer (HGSOC) progression and treatment response. The development and management of cancer depend heavily on CD8+ T cells. Identifying non-invasive predictors of TME immune status is crucial. We investigated whether clinicopathologic characteristics and peripheral blood parameters could predict CD8+ T infiltration in TME of HGSOC. Two independent cohort were analyzed: (1) A multicenter tissue microarray (TMA) cohort of 105 epithelial ovarian cancer cases revealed that high CD8+ T cell density in tumor parenchyma, stroma, or whole tissue was significantly associated with good prognosis. (2) A retrospective cohort of 95 HGSOC patients from West China Second University Hospital (2016–2020) demonstrated that peripheral blood lymphocytes, globulin (GLB), lactate dehydrogenase (LDH), and low-density lipoprotein (LDL) correlated with CD8+ T cell infiltration in TME. These findings support non-invasive blood markers as predictors of tumor immune status and highlight chemotherapy’s role in enhancing CD8+ T cell recruitment.
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spelling doaj-art-973f6727e9e24be08553be12d774c55a2025-08-24T11:25:51ZengNature PortfolioScientific Reports2045-23222025-08-0115111010.1038/s41598-025-14720-7Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patientsJia Mai0Ling Yang1YuXin Chen2XiaoXu Zeng3HongJian Xie4XiaoJuan Liu5Department of Laboratory Medicine, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan University; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationAbstract The tumor microenvironment (TME), particularly CD8+ T cell infiltration, critically influences high-grade serous ovarian cancer (HGSOC) progression and treatment response. The development and management of cancer depend heavily on CD8+ T cells. Identifying non-invasive predictors of TME immune status is crucial. We investigated whether clinicopathologic characteristics and peripheral blood parameters could predict CD8+ T infiltration in TME of HGSOC. Two independent cohort were analyzed: (1) A multicenter tissue microarray (TMA) cohort of 105 epithelial ovarian cancer cases revealed that high CD8+ T cell density in tumor parenchyma, stroma, or whole tissue was significantly associated with good prognosis. (2) A retrospective cohort of 95 HGSOC patients from West China Second University Hospital (2016–2020) demonstrated that peripheral blood lymphocytes, globulin (GLB), lactate dehydrogenase (LDH), and low-density lipoprotein (LDL) correlated with CD8+ T cell infiltration in TME. These findings support non-invasive blood markers as predictors of tumor immune status and highlight chemotherapy’s role in enhancing CD8+ T cell recruitment.https://doi.org/10.1038/s41598-025-14720-7High-grade serous ovarian cancerCD8+ T cellsTumor microenvironmentPeripheral blood parametersPrognosisSpatial distribution
spellingShingle Jia Mai
Ling Yang
YuXin Chen
XiaoXu Zeng
HongJian Xie
XiaoJuan Liu
Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients
Scientific Reports
High-grade serous ovarian cancer
CD8+ T cells
Tumor microenvironment
Peripheral blood parameters
Prognosis
Spatial distribution
title Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients
title_full Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients
title_fullStr Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients
title_full_unstemmed Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients
title_short Prediction of CD8+ T cell infiltration in the tumor microenvironment of HGSOC patients
title_sort prediction of cd8 t cell infiltration in the tumor microenvironment of hgsoc patients
topic High-grade serous ovarian cancer
CD8+ T cells
Tumor microenvironment
Peripheral blood parameters
Prognosis
Spatial distribution
url https://doi.org/10.1038/s41598-025-14720-7
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