Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
Superparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to M...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2007-05-01
|
| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2007.00014 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841563158889627648 |
|---|---|
| author | Joseph A. Frank Heather Kalish E. Kay Jordan Stasia A. Anderson Edyta Pawelczyk Ali S. Arbab |
| author_facet | Joseph A. Frank Heather Kalish E. Kay Jordan Stasia A. Anderson Edyta Pawelczyk Ali S. Arbab |
| author_sort | Joseph A. Frank |
| collection | DOAJ |
| description | Superparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to MRI depends on the ability to detect SPIO-labeled cells using Prussian blue (PB) stain and fluorescent tags to cell surface markers. Exposure of PB-positive sections to ultraviolet light at a wavelength of 365 nm commonly used fluorescence microscopy can result in color transformation of PB-positive material from blue to brown. Although the PB color transformation is primarily an artifact that may occur during fluorescence microscopy, the transformation can be manipulated using imaging process software for the detection of low levels of iron labeled cells in tissues samples |
| format | Article |
| id | doaj-art-972fbf0884a742d19f6664406d3abc33 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2007-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-972fbf0884a742d19f6664406d3abc332025-01-03T00:11:02ZengSAGE PublishingMolecular Imaging1536-01212007-05-01610.2310/7290.2007.0001410.2310_7290.2007.00014Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide NanoparticlesJoseph A. FrankHeather KalishE. Kay JordanStasia A. AndersonEdyta PawelczykAli S. ArbabSuperparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to MRI depends on the ability to detect SPIO-labeled cells using Prussian blue (PB) stain and fluorescent tags to cell surface markers. Exposure of PB-positive sections to ultraviolet light at a wavelength of 365 nm commonly used fluorescence microscopy can result in color transformation of PB-positive material from blue to brown. Although the PB color transformation is primarily an artifact that may occur during fluorescence microscopy, the transformation can be manipulated using imaging process software for the detection of low levels of iron labeled cells in tissues sampleshttps://doi.org/10.2310/7290.2007.00014 |
| spellingShingle | Joseph A. Frank Heather Kalish E. Kay Jordan Stasia A. Anderson Edyta Pawelczyk Ali S. Arbab Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles Molecular Imaging |
| title | Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles |
| title_full | Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles |
| title_fullStr | Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles |
| title_full_unstemmed | Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles |
| title_short | Color Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles |
| title_sort | color transformation and fluorescence of prussian blue positive cells implications for histologic verification of cells labeled with superparamagnetic iron oxide nanoparticles |
| url | https://doi.org/10.2310/7290.2007.00014 |
| work_keys_str_mv | AT josephafrank colortransformationandfluorescenceofprussianbluepositivecellsimplicationsforhistologicverificationofcellslabeledwithsuperparamagneticironoxidenanoparticles AT heatherkalish colortransformationandfluorescenceofprussianbluepositivecellsimplicationsforhistologicverificationofcellslabeledwithsuperparamagneticironoxidenanoparticles AT ekayjordan colortransformationandfluorescenceofprussianbluepositivecellsimplicationsforhistologicverificationofcellslabeledwithsuperparamagneticironoxidenanoparticles AT stasiaaanderson colortransformationandfluorescenceofprussianbluepositivecellsimplicationsforhistologicverificationofcellslabeledwithsuperparamagneticironoxidenanoparticles AT edytapawelczyk colortransformationandfluorescenceofprussianbluepositivecellsimplicationsforhistologicverificationofcellslabeledwithsuperparamagneticironoxidenanoparticles AT alisarbab colortransformationandfluorescenceofprussianbluepositivecellsimplicationsforhistologicverificationofcellslabeledwithsuperparamagneticironoxidenanoparticles |