Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination

This study investigated the influence of soaking and spraying with a sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) solution on selenium accumulation, γ-aminobutyric acid (GABA) content, phenolic compositions, and the antioxidant activity of foxtail millet sprouts. The scree...

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Main Authors: Shuaiduo Sun, Jingjing Zhang, Yongji Li, Yunfeng Xu, Runqiang Yang, Lei Luo, Jinle Xiang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/13/23/3916
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author Shuaiduo Sun
Jingjing Zhang
Yongji Li
Yunfeng Xu
Runqiang Yang
Lei Luo
Jinle Xiang
author_facet Shuaiduo Sun
Jingjing Zhang
Yongji Li
Yunfeng Xu
Runqiang Yang
Lei Luo
Jinle Xiang
author_sort Shuaiduo Sun
collection DOAJ
description This study investigated the influence of soaking and spraying with a sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) solution on selenium accumulation, γ-aminobutyric acid (GABA) content, phenolic compositions, and the antioxidant activity of foxtail millet sprouts. The screening results showed that foxtail millet seeds soaked with 60 mg/L of Na<sub>2</sub>SeO<sub>3</sub> solution and sprayed with 2 mg/L of Na<sub>2</sub>SeO<sub>3</sub> solution were the appropriate concentrations for the germination process. Compared with the spraying method, a presoaking treatment presented far higher selenium content and significantly higher (<i>p</i> < 0.05) selenium enrichment rates in foxtail millet sprouts. The content of free and bound phenolics, as well as GABA, were significantly (<i>p</i> < 0.05) increased in foxtail millet sprouts through both soaking and spraying treatments. Correspondingly, most of the individual phenolic compounds were significantly (<i>p</i> < 0.05) increased, especially after germination for 3 days. <i>Trans</i>-ferulic acid and <i>trans</i>-<i>p</i>-coumaric acid were the predominate bound phenolic acids, feruloylquinic acid and 4-<i>p</i>-coumaroylquinic acid were the major free-form phenolic compounds, and <i>N</i>-feruloyl serotonin and <i>N</i>-(<i>p</i>-coumaroyl) serotonin were the new arising phenolic derivatives caused by germination. Both the soaking and spraying treatments induced the enrichment of these individual phenolic compositions, thus increasing the total phenolic content and in vitro antioxidant activity of foxtail millet sprouts. It was indicated that selenium-enriched germination treatment should be an effective method to produce functional selenium-enriched foxtail millet sprouts with more abundant GABA and polyphenols, thus enhancing the health benefits and added value of foxtail millet.
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institution Kabale University
issn 2304-8158
language English
publishDate 2024-12-01
publisher MDPI AG
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series Foods
spelling doaj-art-972f26d793fb4eb8850bc4dea44e7ec22024-12-13T16:27:01ZengMDPI AGFoods2304-81582024-12-011323391610.3390/foods13233916Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During GerminationShuaiduo Sun0Jingjing Zhang1Yongji Li2Yunfeng Xu3Runqiang Yang4Lei Luo5Jinle Xiang6Faculty of Food & Bioengineering, Henan University of Science & Technology, Luoyang 471023, ChinaFaculty of Food & Bioengineering, Henan University of Science & Technology, Luoyang 471023, ChinaFaculty of Food & Bioengineering, Henan University of Science & Technology, Luoyang 471023, ChinaFaculty of Food & Bioengineering, Henan University of Science & Technology, Luoyang 471023, ChinaCollege of Food Science & Technology, Nanjing Agricultural University, Nanjing 210000, ChinaFaculty of Food & Bioengineering, Henan University of Science & Technology, Luoyang 471023, ChinaFaculty of Food & Bioengineering, Henan University of Science & Technology, Luoyang 471023, ChinaThis study investigated the influence of soaking and spraying with a sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) solution on selenium accumulation, γ-aminobutyric acid (GABA) content, phenolic compositions, and the antioxidant activity of foxtail millet sprouts. The screening results showed that foxtail millet seeds soaked with 60 mg/L of Na<sub>2</sub>SeO<sub>3</sub> solution and sprayed with 2 mg/L of Na<sub>2</sub>SeO<sub>3</sub> solution were the appropriate concentrations for the germination process. Compared with the spraying method, a presoaking treatment presented far higher selenium content and significantly higher (<i>p</i> < 0.05) selenium enrichment rates in foxtail millet sprouts. The content of free and bound phenolics, as well as GABA, were significantly (<i>p</i> < 0.05) increased in foxtail millet sprouts through both soaking and spraying treatments. Correspondingly, most of the individual phenolic compounds were significantly (<i>p</i> < 0.05) increased, especially after germination for 3 days. <i>Trans</i>-ferulic acid and <i>trans</i>-<i>p</i>-coumaric acid were the predominate bound phenolic acids, feruloylquinic acid and 4-<i>p</i>-coumaroylquinic acid were the major free-form phenolic compounds, and <i>N</i>-feruloyl serotonin and <i>N</i>-(<i>p</i>-coumaroyl) serotonin were the new arising phenolic derivatives caused by germination. Both the soaking and spraying treatments induced the enrichment of these individual phenolic compositions, thus increasing the total phenolic content and in vitro antioxidant activity of foxtail millet sprouts. It was indicated that selenium-enriched germination treatment should be an effective method to produce functional selenium-enriched foxtail millet sprouts with more abundant GABA and polyphenols, thus enhancing the health benefits and added value of foxtail millet.https://www.mdpi.com/2304-8158/13/23/3916seleniumfoxtail millet sproutspolyphenolsGABAantioxidant activity
spellingShingle Shuaiduo Sun
Jingjing Zhang
Yongji Li
Yunfeng Xu
Runqiang Yang
Lei Luo
Jinle Xiang
Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination
Foods
selenium
foxtail millet sprouts
polyphenols
GABA
antioxidant activity
title Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination
title_full Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination
title_fullStr Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination
title_full_unstemmed Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination
title_short Effects of Sodium Selenite on Accumulations of Selenium and GABA, Phenolic Profiles, and Antioxidant Activity of Foxtail Millet During Germination
title_sort effects of sodium selenite on accumulations of selenium and gaba phenolic profiles and antioxidant activity of foxtail millet during germination
topic selenium
foxtail millet sprouts
polyphenols
GABA
antioxidant activity
url https://www.mdpi.com/2304-8158/13/23/3916
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