Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction
Although there have been many studies on the efficacy of ultrasonic inactivation, the stress resistance mechanism of bacteria is still a challenge for complete ultrasonic inactivation. In this study, the dominant spoilage bacteria in crayfish, Shewanella baltica (S. baltica) and Aeromonas veronii (A...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724004206 |
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| author | Zechuan Dai Lingyun Meng Sai Wang Jiao Li Xiangzhao Mao |
| author_facet | Zechuan Dai Lingyun Meng Sai Wang Jiao Li Xiangzhao Mao |
| author_sort | Zechuan Dai |
| collection | DOAJ |
| description | Although there have been many studies on the efficacy of ultrasonic inactivation, the stress resistance mechanism of bacteria is still a challenge for complete ultrasonic inactivation. In this study, the dominant spoilage bacteria in crayfish, Shewanella baltica (S. baltica) and Aeromonas veronii (A. veronii), were subjected to high-intensity ultrasonic treatment. The results showed compromised cell membrane, decreased membrane fluidity, hyperpolarized membrane potential, and disrupted succinate-coenzyme Q reductase. Transmission electron microscopy revealed significant fragmentation of S. baltica, whereas A. veronii, with its thick cell wall and outer capsule membrane, demonstrated enhanced resistance to ultrasound. Real-time quantitative PCR indicated that in response to ultrasonic stress, bacteria initiated a stress response mechanism by increasing the expression of mechanosensitive channels; meanwhile, the outer capsule of A. veronii delayed the transformation of ultrasonic external forces into cell membrane stress. The study found that in response to ultrasonic stress, bacteria initiated a stress response mechanism by increasing the expression of mechanosensitive channels as “emergency valve” in short time but could not prevent the process of membrane destruction with prolonged exposure. This finding provided a basis for addressing bacterial stress tolerance in ultrasonic inactivation. |
| format | Article |
| id | doaj-art-971b81f1f4af411da1f88bb96e5c9ecc |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-971b81f1f4af411da1f88bb96e5c9ecc2025-01-11T06:38:43ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107171Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destructionZechuan Dai0Lingyun Meng1Sai Wang2Jiao Li3Xiangzhao Mao4State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR ChinaState Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR ChinaState Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR ChinaState Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR China; Corresponding authors at: State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China.State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, PR China; Qingdao Key Laboratory of Food Biotechnology, Qingdao 266404, PR China; Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR China; Corresponding authors at: State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China.Although there have been many studies on the efficacy of ultrasonic inactivation, the stress resistance mechanism of bacteria is still a challenge for complete ultrasonic inactivation. In this study, the dominant spoilage bacteria in crayfish, Shewanella baltica (S. baltica) and Aeromonas veronii (A. veronii), were subjected to high-intensity ultrasonic treatment. The results showed compromised cell membrane, decreased membrane fluidity, hyperpolarized membrane potential, and disrupted succinate-coenzyme Q reductase. Transmission electron microscopy revealed significant fragmentation of S. baltica, whereas A. veronii, with its thick cell wall and outer capsule membrane, demonstrated enhanced resistance to ultrasound. Real-time quantitative PCR indicated that in response to ultrasonic stress, bacteria initiated a stress response mechanism by increasing the expression of mechanosensitive channels; meanwhile, the outer capsule of A. veronii delayed the transformation of ultrasonic external forces into cell membrane stress. The study found that in response to ultrasonic stress, bacteria initiated a stress response mechanism by increasing the expression of mechanosensitive channels as “emergency valve” in short time but could not prevent the process of membrane destruction with prolonged exposure. This finding provided a basis for addressing bacterial stress tolerance in ultrasonic inactivation.http://www.sciencedirect.com/science/article/pii/S1350417724004206Ultrasonic stressShewanella balticaAeromonas veroniiStress responseMembrane destructionMechanosensitive channels |
| spellingShingle | Zechuan Dai Lingyun Meng Sai Wang Jiao Li Xiangzhao Mao Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction Ultrasonics Sonochemistry Ultrasonic stress Shewanella baltica Aeromonas veronii Stress response Membrane destruction Mechanosensitive channels |
| title | Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction |
| title_full | Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction |
| title_fullStr | Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction |
| title_full_unstemmed | Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction |
| title_short | Dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction |
| title_sort | dominant spoilage bacteria in crayfish alleviate ultrasonic stress through mechanosensitive channels but could not prevent the process of membrane destruction |
| topic | Ultrasonic stress Shewanella baltica Aeromonas veronii Stress response Membrane destruction Mechanosensitive channels |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724004206 |
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