Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis
Abstract C-Repeat Binding Factors (CBFs) are crucial in plant responses to low-temperature stress via the ICE-CBF-COR cascade, but research on these genes in woody and flowering trees remains limited. Hibiscus mutabilis, a woody flowering plant of ornamental and ecological significance, faces low-te...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-05040-x |
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| author | Shiye Sang Yuqiao Zhou Yiqiong Liu |
| author_facet | Shiye Sang Yuqiao Zhou Yiqiong Liu |
| author_sort | Shiye Sang |
| collection | DOAJ |
| description | Abstract C-Repeat Binding Factors (CBFs) are crucial in plant responses to low-temperature stress via the ICE-CBF-COR cascade, but research on these genes in woody and flowering trees remains limited. Hibiscus mutabilis, a woody flowering plant of ornamental and ecological significance, faces low-temperature stress that substantially affects its growth and distribution. Understanding its cold tolerance mechanisms can enhance its utilization and provide insights into plant adaptability to climate change-induced agricultural challenges. This study presents the first genome-wide identification and characterization of the CBF gene family in H. mutabilis. Nine HmCBFs were identified, exhibiting uneven chromosomal distribution and clustering into five phylogenetic clades. Cis-regulatory element analysis indicated potential involvement of HmCBFs in abiotic stress responses and hormone signaling. Homology analysis indicated gene duplication during evolution and a close phylogenetic relationship between H. mutabilis and kenaf. Expression profiling demonstrated higher HmCBF expression in roots than in leaves under normal growth conditions, with significantly increased expression levels at 0 and − 5 °C compared to 5 °C following cold treatment. Our screening of HmCBFs in response to low-temperatures offers valuable insights for breeding cold-tolerant H. mutabilis and contributes to a broader understanding of stress tolerance mechanisms in woody species. |
| format | Article |
| id | doaj-art-96eacf406ea64a6e93e36ff7a66a2620 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-96eacf406ea64a6e93e36ff7a66a26202025-08-20T03:45:20ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-05040-xGenome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilisShiye Sang0Yuqiao Zhou1Yiqiong Liu2Chengdu Botanical Garden (Chengdu Park Urban Institute of Plant Science)International Department of Chengdu Shude High SchoolChengdu Botanical Garden (Chengdu Park Urban Institute of Plant Science)Abstract C-Repeat Binding Factors (CBFs) are crucial in plant responses to low-temperature stress via the ICE-CBF-COR cascade, but research on these genes in woody and flowering trees remains limited. Hibiscus mutabilis, a woody flowering plant of ornamental and ecological significance, faces low-temperature stress that substantially affects its growth and distribution. Understanding its cold tolerance mechanisms can enhance its utilization and provide insights into plant adaptability to climate change-induced agricultural challenges. This study presents the first genome-wide identification and characterization of the CBF gene family in H. mutabilis. Nine HmCBFs were identified, exhibiting uneven chromosomal distribution and clustering into five phylogenetic clades. Cis-regulatory element analysis indicated potential involvement of HmCBFs in abiotic stress responses and hormone signaling. Homology analysis indicated gene duplication during evolution and a close phylogenetic relationship between H. mutabilis and kenaf. Expression profiling demonstrated higher HmCBF expression in roots than in leaves under normal growth conditions, with significantly increased expression levels at 0 and − 5 °C compared to 5 °C following cold treatment. Our screening of HmCBFs in response to low-temperatures offers valuable insights for breeding cold-tolerant H. mutabilis and contributes to a broader understanding of stress tolerance mechanisms in woody species.https://doi.org/10.1038/s41598-025-05040-xHibiscus mutabilisCBFTranscription factorsLow-temperatureExpressionTranscriptome |
| spellingShingle | Shiye Sang Yuqiao Zhou Yiqiong Liu Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis Scientific Reports Hibiscus mutabilis CBF Transcription factors Low-temperature Expression Transcriptome |
| title | Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis |
| title_full | Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis |
| title_fullStr | Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis |
| title_full_unstemmed | Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis |
| title_short | Genome-wide analysis of the CBF gene family and their transcriptional response to cold stress in Hibiscus mutabilis |
| title_sort | genome wide analysis of the cbf gene family and their transcriptional response to cold stress in hibiscus mutabilis |
| topic | Hibiscus mutabilis CBF Transcription factors Low-temperature Expression Transcriptome |
| url | https://doi.org/10.1038/s41598-025-05040-x |
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