Integrating single-cell and spatial transcriptomics reveals the cellular heterogeneity of vestibular schwannoma
Abstract Vestibular schwannoma (VS) is a benign tumor that can result in significant neurological and otological complications. The mechanisms underlying its development and spatial heterogeneity remain poorly understood. In this study, we analyzed single-cell RNA sequencing (scRNA-seq) data from th...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | npj Precision Oncology |
| Online Access: | https://doi.org/10.1038/s41698-025-01028-y |
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| Summary: | Abstract Vestibular schwannoma (VS) is a benign tumor that can result in significant neurological and otological complications. The mechanisms underlying its development and spatial heterogeneity remain poorly understood. In this study, we analyzed single-cell RNA sequencing (scRNA-seq) data from three previously published vestibular schwannomas (VS1-3), along with spatial transcriptomics data from two additional specimens (VS_S1-2). Our results identified a VEGFA-enriched Schwann cell (SC) subtype in scRNA-seq data, which was validated by spatial transcriptomics. This subtype also exhibited a significant positive correlation with NOV hi SCs expression. These cells were centrally localized within tumor tissue. Furthermore, spatial analysis provided new insights into SC-stromal cell interactions, and we constructed a preliminary cellular atlas of VS tissues, enhancing our understanding of tumor growth dynamics. |
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| ISSN: | 2397-768X |