Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing

The Saihanba Mechanical Forest is an artificial national forest park with a forest-steppe landscape. The flora and fauna have been extensively studied, but a comprehensive understanding of the bacterial community composition and structure of the natural rainwater lakes present in the area has rarely...

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Main Authors: Xinrui Gou, Yanli Liu, Kun Li, Chunyan Tian, Jiahao Cui, Shiqi Xu
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Online Access:https://www.tandfonline.com/doi/10.1080/13102818.2024.2326293
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author Xinrui Gou
Yanli Liu
Kun Li
Chunyan Tian
Jiahao Cui
Shiqi Xu
author_facet Xinrui Gou
Yanli Liu
Kun Li
Chunyan Tian
Jiahao Cui
Shiqi Xu
author_sort Xinrui Gou
collection DOAJ
description The Saihanba Mechanical Forest is an artificial national forest park with a forest-steppe landscape. The flora and fauna have been extensively studied, but a comprehensive understanding of the bacterial community composition and structure of the natural rainwater lakes present in the area has rarely been reported. In this study, the structure and functional characteristics of bacterial communities in lake sediments and water samples in the Saihanba artificial forest were investigated using 16s rRNA high-throughput sequencing. Microbial diversity analyses revealed that Proteobacteria, Acidobacteria, Bacteroidota and Verrucomicrobiota microbiota dominated. The abundance of Proteobacteria and Actinobacteriota was significantly higher (p < .05) in water samples compared to sediment samples. PICRUSt2 functional analysis predicted genes associated with the degradation of xenobiotics and the execution of essential metabolic processes. Here, we report differences in the composition of native bacterial communities in sediments and water under the Saihanba artificial forest and make functional gene predictions. This study provides a reference for further exploring the structure and functional characteristics of microbial communities in water samples and sediment environments of lakes under planted forests.
format Article
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issn 1310-2818
1314-3530
language English
publishDate 2024-12-01
publisher Taylor & Francis Group
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series Biotechnology & Biotechnological Equipment
spelling doaj-art-9687153595fe4321a80b41cf468f2bd92024-12-10T05:42:51ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302024-12-0138110.1080/13102818.2024.2326293Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencingXinrui Gou0Yanli Liu1Kun Li2Chunyan Tian3Jiahao Cui4Shiqi Xu5Department of Biomedical Engineering, Chengde Medical University, Chengde, PR ChinaDepartment of Biomedical Engineering, Chengde Medical University, Chengde, PR ChinaDepartment of Biomedical Engineering, Chengde Medical University, Chengde, PR ChinaDepartment of Biomedical Engineering, Chengde Medical University, Chengde, PR ChinaDepartment of Biomedical Engineering, Chengde Medical University, Chengde, PR ChinaDepartment of Biomedical Engineering, Chengde Medical University, Chengde, PR ChinaThe Saihanba Mechanical Forest is an artificial national forest park with a forest-steppe landscape. The flora and fauna have been extensively studied, but a comprehensive understanding of the bacterial community composition and structure of the natural rainwater lakes present in the area has rarely been reported. In this study, the structure and functional characteristics of bacterial communities in lake sediments and water samples in the Saihanba artificial forest were investigated using 16s rRNA high-throughput sequencing. Microbial diversity analyses revealed that Proteobacteria, Acidobacteria, Bacteroidota and Verrucomicrobiota microbiota dominated. The abundance of Proteobacteria and Actinobacteriota was significantly higher (p < .05) in water samples compared to sediment samples. PICRUSt2 functional analysis predicted genes associated with the degradation of xenobiotics and the execution of essential metabolic processes. Here, we report differences in the composition of native bacterial communities in sediments and water under the Saihanba artificial forest and make functional gene predictions. This study provides a reference for further exploring the structure and functional characteristics of microbial communities in water samples and sediment environments of lakes under planted forests.https://www.tandfonline.com/doi/10.1080/13102818.2024.2326293Microbial communitytaxonomyfunction16S rRNAartificial forestlake
spellingShingle Xinrui Gou
Yanli Liu
Kun Li
Chunyan Tian
Jiahao Cui
Shiqi Xu
Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing
Biotechnology & Biotechnological Equipment
Microbial community
taxonomy
function
16S rRNA
artificial forest
lake
title Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing
title_full Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing
title_fullStr Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing
title_full_unstemmed Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing
title_short Bacterial diversity and functional characteristics of natural rainwater lakes in Saihanba artificial forest farm as revealed by 16S rRNA sequencing
title_sort bacterial diversity and functional characteristics of natural rainwater lakes in saihanba artificial forest farm as revealed by 16s rrna sequencing
topic Microbial community
taxonomy
function
16S rRNA
artificial forest
lake
url https://www.tandfonline.com/doi/10.1080/13102818.2024.2326293
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