Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq

Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq

Summary: RNA 5-methylcytosine (m5C) is a widespread modification and plays a crucial role in gene expression regulation. Here, we present a protocol for transcriptome-wide m5C methylome profiling at base resolution using bisulfite-free m5C detection strategy enabled by ten-eleven translocation (TET)...

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Bibliographic Details
Main Authors: Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Genetics
Genomics
Sequencing
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500005X
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