Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals
Abstract Cholesterol crystals, which cause inflammation and various diseases, predominantly grow in a platy, rhomboid structure on the plasma membranes but exhibit an uneven three‐dimensional (3D) architecture intracellularly. Here, it is demonstrated how cholesterol crystallizes in a non‐rhomboidal...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202409201 |
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| author | Hyun‐Ro Lee Seunghan Kang Siyoung Q. Choi |
| author_facet | Hyun‐Ro Lee Seunghan Kang Siyoung Q. Choi |
| author_sort | Hyun‐Ro Lee |
| collection | DOAJ |
| description | Abstract Cholesterol crystals, which cause inflammation and various diseases, predominantly grow in a platy, rhomboid structure on the plasma membranes but exhibit an uneven three‐dimensional (3D) architecture intracellularly. Here, it is demonstrated how cholesterol crystallizes in a non‐rhomboidal shape on the surface of lipid droplets and develops into 3D sheet‐like agglomerates using an in vitro lipid droplet reconstitution system with stereoscopic fluorescence imaging. The findings reveal that interfacial cholesterol transport on the lipid droplet surface and unique lipid droplet components significantly influence the nucleation‐and‐growth dynamics of cholesterol crystals, leading to crystal growth in various polygonal shapes. Furthermore, cholesterol crystals readily agglomerate to form large, curved sheet structures on the confined, spherical surfaces of lipid droplets. This discovery enhances the understanding of the volumetric morphological growth of intracellular cholesterol crystals. |
| format | Article |
| id | doaj-art-9667f1e4bb5247908798f4e779262d51 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-9667f1e4bb5247908798f4e779262d512025-01-09T11:44:46ZengWileyAdvanced Science2198-38442025-01-01121n/an/a10.1002/advs.202409201Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol CrystalsHyun‐Ro Lee0Seunghan Kang1Siyoung Q. Choi2Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon 34141 Republic of KoreaDepartment of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon 34141 Republic of KoreaDepartment of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon 34141 Republic of KoreaAbstract Cholesterol crystals, which cause inflammation and various diseases, predominantly grow in a platy, rhomboid structure on the plasma membranes but exhibit an uneven three‐dimensional (3D) architecture intracellularly. Here, it is demonstrated how cholesterol crystallizes in a non‐rhomboidal shape on the surface of lipid droplets and develops into 3D sheet‐like agglomerates using an in vitro lipid droplet reconstitution system with stereoscopic fluorescence imaging. The findings reveal that interfacial cholesterol transport on the lipid droplet surface and unique lipid droplet components significantly influence the nucleation‐and‐growth dynamics of cholesterol crystals, leading to crystal growth in various polygonal shapes. Furthermore, cholesterol crystals readily agglomerate to form large, curved sheet structures on the confined, spherical surfaces of lipid droplets. This discovery enhances the understanding of the volumetric morphological growth of intracellular cholesterol crystals.https://doi.org/10.1002/advs.202409201cholesterolcrystal morphologycrystallizationinterfacial phenomenalipid droplets |
| spellingShingle | Hyun‐Ro Lee Seunghan Kang Siyoung Q. Choi Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals Advanced Science cholesterol crystal morphology crystallization interfacial phenomena lipid droplets |
| title | Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals |
| title_full | Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals |
| title_fullStr | Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals |
| title_full_unstemmed | Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals |
| title_short | Lipid Droplet Surface Promotes 3D Morphological Evolution of Non‐Rhomboidal Cholesterol Crystals |
| title_sort | lipid droplet surface promotes 3d morphological evolution of non rhomboidal cholesterol crystals |
| topic | cholesterol crystal morphology crystallization interfacial phenomena lipid droplets |
| url | https://doi.org/10.1002/advs.202409201 |
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