Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells
Most studies on anesthesia focus on the nervous system of mammals due to their interest in medicine. The fact that any life form can be anaesthetised is often overlooked although anesthesia targets ion channel activities that exist in all living beings. This study examines the impact of lidocaine on...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Plant Signaling & Behavior |
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| Online Access: | http://dx.doi.org/10.1080/15592324.2024.2388443 |
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| _version_ | 1846095035523661824 |
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| author | Lucia Sylvain Bonfanti Delphine Arbelet-Bonnin Frédéric Filaine Christophe Lalanne Aurélien Renault Patrice Meimoun Patrick Laurenti Etienne Grésillon François Bouteau |
| author_facet | Lucia Sylvain Bonfanti Delphine Arbelet-Bonnin Frédéric Filaine Christophe Lalanne Aurélien Renault Patrice Meimoun Patrick Laurenti Etienne Grésillon François Bouteau |
| author_sort | Lucia Sylvain Bonfanti |
| collection | DOAJ |
| description | Most studies on anesthesia focus on the nervous system of mammals due to their interest in medicine. The fact that any life form can be anaesthetised is often overlooked although anesthesia targets ion channel activities that exist in all living beings. This study examines the impact of lidocaine on rice (Oryza sativa). It reveals that the cellular responses observed in rice are analogous to those documented in animals, encompassing direct effects, the inhibition of cellular responses, and the long-distance transmission of electrical signals. We show that in rice cells, lidocaine has a cytotoxic effect at a concentration of 1%, since it induces programmed reactive oxygen species (ROS) and caspase-like-dependent cell death, as already demonstrated in animal cells. Additionally, lidocaine causes changes in membrane ion conductance and induces a sharp reduction in electrical long-distance signaling following seedlings leaves burning. Finally, lidocaine was shown to inhibit osmotic stress-induced cell death and the regulation of Ca2+ homeostasis. Thus, lidocaine treatment in rice and tobacco (Nicotiana benthamiana) seedlings induces not only cellular but also systemic effects similar to those induced in mammals. |
| format | Article |
| id | doaj-art-965f9719f3684d45817409f0aeb792b9 |
| institution | Kabale University |
| issn | 1559-2316 1559-2324 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj-art-965f9719f3684d45817409f0aeb792b92025-01-02T11:44:27ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242024-12-0119110.1080/15592324.2024.23884432388443Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cellsLucia Sylvain Bonfanti0Delphine Arbelet-Bonnin1Frédéric Filaine2Christophe Lalanne3Aurélien Renault4Patrice Meimoun5Patrick Laurenti6Etienne Grésillon7François Bouteau8Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Laboratoire Dynamiques sociales et recomposition des espaces (LADYSS UMR 7533)Laboratoire Interdisciplinaire des Énergies de Demain (LIED)Most studies on anesthesia focus on the nervous system of mammals due to their interest in medicine. The fact that any life form can be anaesthetised is often overlooked although anesthesia targets ion channel activities that exist in all living beings. This study examines the impact of lidocaine on rice (Oryza sativa). It reveals that the cellular responses observed in rice are analogous to those documented in animals, encompassing direct effects, the inhibition of cellular responses, and the long-distance transmission of electrical signals. We show that in rice cells, lidocaine has a cytotoxic effect at a concentration of 1%, since it induces programmed reactive oxygen species (ROS) and caspase-like-dependent cell death, as already demonstrated in animal cells. Additionally, lidocaine causes changes in membrane ion conductance and induces a sharp reduction in electrical long-distance signaling following seedlings leaves burning. Finally, lidocaine was shown to inhibit osmotic stress-induced cell death and the regulation of Ca2+ homeostasis. Thus, lidocaine treatment in rice and tobacco (Nicotiana benthamiana) seedlings induces not only cellular but also systemic effects similar to those induced in mammals.http://dx.doi.org/10.1080/15592324.2024.2388443anaesthesiacalciumlidocaineosmotic stressprogrammed cell deathrice |
| spellingShingle | Lucia Sylvain Bonfanti Delphine Arbelet-Bonnin Frédéric Filaine Christophe Lalanne Aurélien Renault Patrice Meimoun Patrick Laurenti Etienne Grésillon François Bouteau Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells Plant Signaling & Behavior anaesthesia calcium lidocaine osmotic stress programmed cell death rice |
| title | Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells |
| title_full | Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells |
| title_fullStr | Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells |
| title_full_unstemmed | Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells |
| title_short | Toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells |
| title_sort | toxic and signaling effects of the anaesthetic lidocaine on rice cultured cells |
| topic | anaesthesia calcium lidocaine osmotic stress programmed cell death rice |
| url | http://dx.doi.org/10.1080/15592324.2024.2388443 |
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