Protocol for live neuron imaging analysis of basal surface fraction and dynamic availability of the dopamine transporter using DAT-pHluorin

Protocol for live neuron imaging analysis of basal surface fraction and dynamic availability of the dopamine transporter using DAT-pHluorin

Summary: Surface availability of the dopamine (DA) transporter (DAT) critically influences DA transmission. Here, we present a protocol that describes the preparation of mouse ventral midbrain neurons, the expression of a new optical sensor, DAT-pHluorin, and the utilization of this sensor to analyz...

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Bibliographic Details
Main Authors: Elnaz Khezerlou, Jacqueline Saenz, Sanjana Surya Prakash, Ping-Yue Pan
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
cell biology
cell culture
cell-based assays
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005239
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